Reactive Oxygen Species-Responsive Gel-Based Microneedle Patches with Antimicrobial and Immunomodulating Properties for Oral Mucosa Disease Treatment DOI
Xuancheng Zhang,

Guannan Zhong,

Shiyuan Peng

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Oral ulcer wounds are difficult to heal due bacterial infections, persistent inflammatory responses, and excessive reactive oxygen species (ROS). Therefore, the elimination of bacteria, removal ROS, reduction inflammation prerequisites for treatment mouth wounds. In this study, oligomeric proanthocyanidins (OPC) 3-(aminomethyl)phenylboronic acid-modified hyaluronic acid (HP) were used form polymer gels through dynamic covalent borate bonds. Minocycline hydrochloride (MH) was then loaded into gel, a multifunctional MH/OPC-HP microneedles (MNs) with ROS-responsive properties prepared using vacuum method. The MNs can rapidly release MH in diffusive manner sustainably OPC response ROS. gel-based extended retention oral ulcers, leading prolonged ROS scavenging effects. Cytocompatibility hemocompatibility tests showed that had good biocompatibility. Antibacterial experiments demonstrated exhibited excellent antibacterial vitro indicated could effectively clear reduce oxidative stress damage, inhibit M1-type macrophage polarization, induce M2-type polarization. Furthermore, vivo revealed pro-inflammatory cytokines, promote neovascularization, accelerate epithelial healing significantly rat model wound infection. summary, hold promise as therapeutic strategy enhancing

Language: Английский

Programmed microalgae-gel promotes chronic wound healing in diabetes DOI Creative Commons
Yong Kang, Lingling Xu,

Jinrui Dong

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 3, 2024

Abstract Chronic diabetic wounds are at lifelong risk of developing foot ulcers owing to severe hypoxia, excessive reactive oxygen species (ROS), a complex inflammatory microenvironment, and the potential for bacterial infection. Here we develop programmed treatment strategy employing live Haematococcus (HEA). By modulating light intensity, HEA can be perform variety functions, such as antibacterial activity, supply, ROS scavenging, immune regulation, suggesting its use in therapy. Under high intensity (658 nm, 0.5 W/cm 2 ), green (GHEA) with efficient photothermal conversion mediate wound surface disinfection. decreasing 0.1 photosynthetic system GHEA continuously produce oxygen, effectively resolving problems hypoxia promoting vascular regeneration. Continuous irradiation induces astaxanthin (AST) accumulation cells, resulting gradual transformation from red hue (RHEA). RHEA scavenges excess ROS, enhances expression intracellular antioxidant enzymes, directs polarization M2 macrophages by secreting AST vesicles via exosomes. The living hydrogel sterilize enhance cell proliferation migration promote neoangiogenesis, which could improve infected healing female mice.

Language: Английский

Citations

98
An Immunoregulation Hydrogel with Controlled Hyperthermia‐Augmented Oxygenation and ROS Scavenging for Treating Diabetic Foot Ulcers DOI
Xiaoliang Qi,

XinXin Ge,

Xiaojing Chen

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(33)

Published: May 6, 2024

Abstract Diabetic foot ulcers (DFUs), a serious and increasingly common chronic issue among diabetics, often do not respond well to generalized treatment strategies. Hypoxia the overexpression of reactive oxygen species (ROS), resulting in inflammatory dysregulation subsequent imbalance macrophage phenotypes, are critical factors contributing prolonged non‐healing DFU wounds. These two issues interact continuous, problematic cycle. Presently, there is lack comprehensive strategies aimed at addressing both these simultaneously interrupt this detrimental Herein, an immunomodulatory hydrogel (PHG2) developed for reshaping hostile microenvironment. The engineered PHG2 only removes excess internally‐produced ROS but also generates O 2 , with its efficiency further boosted by local hyperthermia (42.5 °C) activated near‐infrared light. Through vitro vivo studies, along transcriptomic assessment, it confirmed that disrupts feedback loop between inflammation while lowering M1/M2 ratio. Such discoveries contribute significant enhancement healing process injuries undergo gradual increase movement, covering wounds from back, mouth, foot. Ultimately, method provides easy, safe, highly effective solution treating DFUs.

Language: Английский

Citations

70
A Hybrid Hydrogel with Intrinsic Immunomodulatory Functionality for Treating Multidrug-Resistant Pseudomonas aeruginosa Infected Diabetic Foot Ulcers DOI
Xiaoliang Qi,

Yizuo Shi,

Chaofan Zhang

et al.

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(7), P. 2533 - 2547

Published: May 24, 2024

Contemporary options for multidrug-resistant bacteria infected diabetic foot ulcers (IDFUs) are predominantly nonspecific. These IDFU injuries often display prolonged inflammation and delayed tissue repair, mainly attributed to an overabundance of M1 macrophages in the hostile microenvironment. Although immunomodulatory hydrogels show promise IDFU-focused care, a targeted, safe transition from M2 using simplified techniques remains significant obstacle. Here, we introduce hybrid hydrogel (GGG) with inherent capabilities IDFUs. GGG is composed interpenetrating polymer networks featuring gallium-induced self-assembling glycyrrhizic acid photo-cross-linked gelatin methacryloyl matrix. Importantly, effectively decreases ratio conditions drug-resistant by disrupting iron metabolism scavenging reactive oxygen species, which contributes enhanced treatment results wounds. To sum up, strategy present provides straightforward, safe, highly effective therapeutic avenue managing

Language: Английский

Citations

67
Designing nanozymes for in vivo applications DOI
Ruofei Zhang, Bing Jiang, Kelong Fan

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 849 - 868

Published: July 18, 2024

Language: Английский

Citations

54
Intelligent biobased hydrogels for diabetic wound healing: A review DOI
Hanzhang Wang, Liming Zhang

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 484, P. 149493 - 149493

Published: Feb. 10, 2024

Language: Английский

Citations

52
Phenolic Ligand–Metal Charge Transfer Induced Copper Nanozyme with Reactive Oxygen Species-Scavenging Ability for Chronic Wound Healing DOI
Yi Chen,

Xiaoru Yang,

Kai Li

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(9), P. 7024 - 7036

Published: Feb. 23, 2024

Chronic wounds frequently arise as a complication in diabetic patients, and their management remains significant clinical hurdle due to nonhealing nature featured by heightened oxidative stress impaired healing cells at the wound site. Herein, we present 2D copper antioxidant nanozyme induced phenolic ligand–metal charge transfer (LMCT) eliminate reactive oxygen species (ROS) facilitate of chronic wounds. We found that polyphenol ligands coordinated on Cu3(PO4)2 nanosheets led strong interface regulated valence states Cu. The obtained Cu exhibited efficient scavenging ability toward different protected human from damage. enhanced promoting re-epithelialization, collagen deposition, angiogenesis, immunoregulation. This work demonstrates LMCT-induced ROS nanointerface, providing an alternative strategy constructing metal-based nanozymes for treatment well other diseases.

Language: Английский

Citations

35
Glucose-Responsive hydrogel optimizing Fenton reaction to eradicate multidrug-resistant bacteria for infected diabetic wound healing DOI
Xingchen Li, Zi-Fan Meng,

Lin Guan

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 487, P. 150545 - 150545

Published: March 19, 2024

Language: Английский

Citations

25
Polyphenol‐Copper Derived Self‐Cascade Nanozyme Hydrogel in Boosting Oxygenation and Robust Revascularization for Tissue Regeneration DOI

Mengyao Wen,

Tianyi Wang, Na Li

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(40)

Published: April 25, 2024

Abstract The regeneration of hypoxia‐impaired chronic tissue defects has long been challenging, mainly due to the inefficiency oxygenation and limited biological activity existing oxygen delivery systems in regulating dynamic process. Herein, a novel polyphenol‐copper coordination strategy fabricate bioactive superoxide dismutase‐catalase self‐cascade nanozymes (SalB‐CuNCs) is reported, which can serve as an situ oxygenator induce angiogenesis simultaneously. copper‐phenolic hydroxyl structure SalB‐CuNCs plays critical role promoting enzyme‐like cascade reaction via catechol‐mediated Cu valence state transition substrate capture mechanism. Furthermore, after incorporating into Schiff base hydrogel (COC@SalB‐Cu), resulting system exhibits outstanding antioxidant robust effect mitigating hypoxic microenvironment. Benefiting from intrinsic angiogenic SalB copper, COC@SalB‐Cu more complete tube formation by up‐regulating expression level vascular endothelial growth factor (VEGF), platelet‐endothelial cell adhesion molecule‐1 (CD31), nitric oxide synthase (eNOS). In vivo experiments further demonstrate that significantly restore blood supply, leading fast regeneration. present holds enormous promise for treatment hypoxia‐related injury field regenerative medicine.

Language: Английский

Citations

20
Extracellular Matrix-Mimetic Intrinsic Versatile Coating Derived from Marine Adhesive Protein Promotes Diabetic Wound Healing through Regulating the Microenvironment DOI
Lulu Wang, Xue Bo, Xin Zhang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(22), P. 14726 - 14741

Published: May 22, 2024

The management of diabetic wound healing remains a severe clinical challenge due to the complicated microenvironments, including abnormal immune regulation, excessive reactive oxygen species (ROS), and repeated bacterial infections. Herein, we report an extracellular matrix (ECM)-mimetic coating derived from scallop byssal protein (Sbp9Δ), which can be assembled in situ within 30 min under trigger Ca2+ driven by strong coordination interaction. biocompatible Sbp9Δ genetically programmable LL37-fused exhibit outstanding antioxidant, antibacterial, regulatory properties vitro. Proof-of-concept applications demonstrate that reliably promote animal models, mice rabbits, ex vivo human skins, Staphylococcus aureus-infected mice. In-depth mechanism investigation indicates improved microenvironments accelerated repair, alleviated infection, lessened inflammation, appearance abundant M2-type macrophages, removal ROS, promoted angiogenesis, re-epithelialization. Collectively, our provides situ, convenient, effective approach for repair.

Language: Английский

Citations

19
Bioactive hydrogel formulations for regeneration of pathological bone defects DOI
Zuhao Li, Kaixuan Ren, Jiajia Chen

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 686 - 714

Published: Feb. 17, 2025

Language: Английский

Citations

2