Protein Corona of Nanoparticles: Isolation and Analysis

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 1(9), P. 757 - 772

Published: Oct. 3, 2024

Nanoparticles entering biological systems or fluids inevitably adsorb biomolecules, such as protein, on their surfaces, forming a protein corona. Ensuing, the corona endows nanoparticles with new identity and impacts interaction between systems. Hence, development of reliable techniques for isolation analysis is key understanding behaviors nanoparticles. First, this review systematically outlines approach isolating corona, including centrifugation, magnetic separation, size exclusion chromatography, flow-field-flow fractionation, other emerging methods. Next, we qualitative quantitative characterization methods Finally, underscore necessary steps to advance efficiency fidelity nanoparticle surfaces. We anticipate that these insights into methodologies will profoundly influence technologies aimed at elucidating bionano interactions role in various biomedical applications.

Language: Английский

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Disease‐Adaptive Drug Delivery to the Inflamed Intestinal Mucosa Using Poly(Lactic‐Co‐Glycolic Acid)‐cyclodextrin Hybrid Nanocarriers

Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract Fluctuating severity of symptoms is a common hallmark many inflammatory disorders, including bowel disease (IBD). Addressing the pH changes during active and resting phases in IBD‐affected tissue, disease‐adaptive nanocarrier system designed for oral administration, enabling pH‐dependent local drug release. The hybrid carrier combines poly(lactic‐co‐glycolic acid) an amphiphilic cyclodextrin derivative, with physicochemical properties release kinetics controlled by adjusting polymer ratios. systems exhibited baseline at 5 increased rates 2, which characteristic actively inflamed IBD tissue. Assessing impact biomolecule adhesion, biocorona formation was studied using ex vivo human intestinal fluids. Corona composition highly depended on patient's prandial state matrix, proteins predominating fasted lipids fed state. Notably, differences attachment free fatty acids are detected latter. Transport studies vitro models intestine revealed mucosal accumulation, facilitating localized delivery effectively reducing cytokine levels to basal concentrations. This highlights potential treatment underscores therapeutic performance gastrointestinal tract.

Language: Английский

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Micro- and Nanomotors: Engineered Tools for Targeted and Efficient Biomedicine

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Over the past two decades, nanotechnology has made significant progress toward development and applications of micromotors (MMs) nanomotors (NMs). Characterized by their capability to self-propel swim in fluids, they have emerged as promising tools various fields, particularly biomedicine. This Review presents an overview current state MMs NMs, motion viscous media complex environments, interaction with biological barriers, potential therapeutical applications. We identify choice appropriate administration routes reach target location a key aspect success NMs biomedical Looking ahead, we envision playing role treating diverse medical disorders, recent proof-of-concept vivo studies demonstrate distinct capabilities versatility. However, addressing regulatory, scalability, biocompatibility, safety concerns remains imperative for successful translation into clinical trials industrial-scale production. work provides guideline researchers, guiding them through landscape, challenges, prospects using biomedicine, thereby encouraging responsible positioning future nanomedicine. Furthermore, outline critical areas further research, including on safety, methods overcome physical obstacles.

Language: Английский

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Unraveling the protein Corona formation during the surface-enhanced Raman spectroscopy detection of malachite green in fish

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144474 - 144474

Published: April 1, 2025

Language: Английский

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Debugging the dynamics of protein corona: Formation, composition, challenges, and applications at the nano-bio interface

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 342, P. 103535 - 103535

Published: May 1, 2025

Language: Английский

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Biomolecular Corona of Gold Nanoparticles: The Urgent Need for Strong Roots to Grow Strong Branches

Small, Journal Year: 2023, Volume and Issue: 20(15)

Published: Dec. 12, 2023

Gold nanoparticles (GNPs) are largely employed in diagnostics/biosensors and among the most investigated nanomaterials biology/medicine. However, few GNP-based nanoformulations have received FDA approval to date, promising vitro studies failed translate vivo efficacy. One key factor is that biological fluids contain high concentrations of proteins, lipids, sugars, metabolites, which can adsorb/interact with GNP's surface, forming a layer called biomolecular corona (BMC). The BMC mask prepared functionalities target moieties, creating new surface chemistry determining GNPs' fate. Here, current knowledge summarized on GNP-BMCs, analyzing factors driving these interactions consequences. A partial fingerprint GNP-BMC common patterns composition literature extrapolated. red flag also risen concerning lack data availability regulated form BMC. Nanomedicine still its infancy, relying recently developed analytical informatic tools offers an unprecedented opportunity make leap forward. restart through robust shared protocols sharing necessary obtain "stronger roots". This will create path exploiting for human benefit, promoting clinical translation biomedical nanotools.

Language: Английский

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In Situ Measurement of Nanoparticle–Blood Protein Adsorption and Its Heterogeneity with Single-Nanoparticle Resolution via Dual Fluorescence Quantification

Nano Letters, Journal Year: 2024, Volume and Issue: 24(30), P. 9202 - 9211

Published: July 22, 2024

The formation of a protein corona gives nanomedicines distinct biological identity, profoundly influencing their fate in the body. Nonspecific nanoparticle–protein interactions are typically highly heterogeneous, which can lead to unique behaviors and vivo fates for individual nanoparticles that remain underexplored. To address this, we have established an situ approach allows quantitative examination adsorption at nanoparticle level. This method integrates dual fluorescence quantification techniques, wherein first individually analyzed via nanoflow cytometry detect fluorescent signals from adsorbed proteins. obtained intensity is then translated into quantities through calibration with microplate reader quantification. Consequently, this enables analysis interparticle heterogeneity nano–protein interactions, as well monitoring kinetics aggregation status blood serum, preconditioning comprehensive understanding nano–bio predicting nanomedicines.

Language: Английский

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Super-Resolution Microscopy as a Versatile Tool in Probing Molecular Assembly

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11497 - 11497

Published: Oct. 26, 2024

Molecular assembly is promising in the construction of advanced materials, obtaining structures with specific functions. In-depth investigation relationships between formation, dynamics, structure, and functionality molecular assemblies one greatest challenges nanotechnology chemistry, which essential rational design development functional materials for a variety applications. Super-resolution microscopy (SRM) has been used as versatile tool investigating elucidating individual its nanometric resolution, multicolor ability, minimal invasiveness, are also complementary to conventional optical or electronic techniques that provide direct observation. In this review, we will an overview representative studies utilize SRM probe assemblies, mainly focusing on imaging biomolecular (lipid-based, peptide-based, protein-based, DNA-based), organic–inorganic hybrid polymer assemblies. This review guidelines evaluation dynamics disassembly processes distinct dynamic behaviors, multicomponent through application these techniques. We believe inspire new ideas propel structural analyses promote exploitation new-generation materials.

Language: Английский

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An Investigation of the Protein Corona in the Cellular Uptake of Nanoliposomes Under Flow Conditions Using Quartz Crystal Microbalance with Dissipation

Nanoscale Advances, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The investigation of the effect protein corona on cellular uptake nanoliposome formulations in real-time under flow conditions by quartz crystal microbalance with dissipation as a biomimetic vitro technique.

Language: Английский

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Probing the Cellular Fate of the Protein Corona around Nanoparticles with Nanofocused X-ray Fluorescence Imaging

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 528 - 528

Published: Dec. 30, 2023

X-ray fluorescence imaging (XRF-imaging) with subcellular resolution is used to study the intracellular integrity of a protein corona that was pre-formed around gold nanoparticles (AuNP). Artificial proteins engineered obtain Gd coordination for detection by XRF-imaging were form corona. Indications about degradation this at cellular and level can be observed following Au quantities in time spatial-dependent manner. The extended acquisition times necessary capturing individual cell images result relatively small sample populations, stressing need faster image strategies future XRF-imaging-based studies deal inherent variability between cells. Still, results obtained reveal during trafficking, followed differential processing AuNP Gd-labelled proteins. Overall, demonstrates dynamic tracked certain degree.

Language: Английский

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