Screening Oxidoreductase Activities of Nanozymes: Toward Activity‐Tailored Guidelines DOI Creative Commons
Giulia Mirra, Lorenzo Cursi, Luca Boselli

et al.

Small Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Nanozymes (NZs) are nanomaterials able to mimic natural enzymes, offering the advantages of better durability, wider range operational conditions, and easier functionalization. In addition, some NZs exhibit multiple enzyme‐like activities effective tandem/self‐cascade reactions. this context, metallic metal–oxide nanoparticles among most promising candidates for biomedical/biotechnological applications, thanks their efficient oxidoreductase activities. However, characterizing is challenging because experimental set‐up different assays strongly influence performances. Consequently, currently available literature provides limited understanding characteristics various NZs, especially in terms activity‐related performance, hindering optimal selection applicative potential. Here, leveraging on accurate characterization methodology, we report a direct comparison several (gold‐Au, platinum‐Pt, palladium‐Pd, ceria, iron oxide) possessing multi‐enzymatic Our main findings indicate that 1) PtNZs outperform other tested catalase‐like activity; 2) Pt PdNZs present superior superoxide dismutase‐like 3) peroxidase‐ oxidase‐like activity, best‐performing NZ substrate‐dependent; 4) competitive reactions multifunctional play key role specific AuNZs can be choice peroxidase/oxidase configurations; 5) more than metal‐oxide ones, but 6) ceria presents unique phosphatase‐like activity.

Language: Английский

NIR-enhanced Pt single atom/g-C3N4 nanozymes as SOD/CAT mimics to rescue ATP energy crisis by regulating oxidative phosphorylation pathway for delaying osteoarthritis progression DOI Creative Commons

Jianhui Xiang,

Xin Yang,

Manli Tan

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 1 - 13

Published: Feb. 21, 2024

Osteoarthritis (OA) progresses due to the excessive generation of reactive oxygen and nitrogen species (ROS/RNS) abnormal ATP energy metabolism related oxidative phosphorylation pathway in mitochondria. Highly active single-atom nanozymes (SAzymes) can help regulate redox balance have shown their potential treatment inflammatory diseases. In this study, we innovatively utilised ligand-mediated strategies chelate Pt

Language: Английский

Citations

33
Frontier applications of retinal nanomedicine: progress, challenges and perspectives DOI Creative Commons

Zhimin Tang,

Fuxiang Ye, Ni Ni

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 25, 2025

The human retina is a fragile and sophisticated light-sensitive tissue in the central nervous system. Unhealthy retinas can cause irreversible visual deterioration permanent vision loss. Effective therapeutic strategies are restricted to treatment or reversal of these conditions. In recent years, nanoscience nanotechnology have revolutionized targeted management retinal diseases. Pharmaceuticals, theranostics, regenerative medicine, gene therapy, prostheses indispensable for interventions been significantly advanced by nanomedical innovations. Hence, this review presents novel insights into use versatile nanomaterial-based nanocomposites frontier applications, including non-invasive drug delivery, theranostic contrast agents, nanoagents, stem cell-based optogenetics prostheses, which mainly reported within last 5 years. Furthermore, progress, potential challenges, future perspectives field highlighted discussed detail, may shed light on clinical translations ultimately, benefit patients with disorders.

Language: Английский

Citations

2
Choline Phosphate-Grafted Nanozymes as Universal Extracellular Vesicle Probes for Bladder Cancer Detection DOI
Qiang Li,

Saisong Zhan,

Xiaoqing Yang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(25), P. 16113 - 16125

Published: June 10, 2024

Urinary extracellular vesicles (uEVs) are regarded as highly promising liquid-biopsy biomarkers for the early diagnosis and prognosis of bladder cancer (BC). However, detection uEVs remains technically challenging owing to their huge heterogeneity ultralow abundance in real samples. We herein present a choline phosphate-grafted platinum nanozyme (Pt@CP) that acts universal EV probe construction high-throughput high-sensitivity immunoassay, which allowed multiplex profiling uEV protein markers BC detection. With Pt@CP-based immunoassays, three (MUC-1, CCDC25, GLUT1) were identified BC, by cases (n = 48), cystitis patients 27), healthy donors 24) discriminated with high clinical sensitivity specificity (area under curve 98.3%). For 9) after surgery, immunoassay could report postoperative residual tumor cannot be observed cystoscopy, is clinically significant assessing recurrence. This work provides generally detection, facilitating discovery use EV-based biomarkers.

Language: Английский

Citations

17
Recent Advances in Nanomedicine for Ocular Fundus Neovascularization Disease Management DOI Creative Commons
Yifan Zhou, Mingyu Xu, Wenyue Shen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(17)

Published: Feb. 26, 2024

As an indispensable part of the human sensory system, visual acuity may be impaired and even develop into irreversible blindness due to various ocular pathologies. Among diseases, fundus neovascularization diseases (FNDs) are prominent etiologies impairment worldwide. Intravitreal injection anti-vascular endothelial growth factor drugs remains primary therapy but is hurdled by common complications incomplete potency. To renovate current therapeutic modalities, nanomedicine emerged as times required, which endowed with advanced capabilities, able fulfill effective drug delivery achieve precise release control, thus further improving effect. This review provides a comprehensive summary advances in for FND management from state-of-the-art studies. First, modalities FNDs thoroughly introduced, focusing on key challenges delivery. Second, nanocarriers comprehensively reviewed posterior based nanostructures: polymer-based nanocarriers, lipid-based inorganic nanoparticles. Thirdly, characteristics microenvironment, their pathological changes during FNDs, corresponding strategies constructing smart elaborated. Furthermore, prospects discussed.

Language: Английский

Citations

11
Co-delivery of antioxidants and siRNA-VEGF: promising treatment for age-related macular degeneration DOI
Marina França Dias, Estael Luzia Coelho da Cruz-Cazarim, Frederico Pittella

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

Citations

1
Nanozymes for Treating Ocular Diseases DOI
Ka‐Ying Wong, Man‐Sau Wong, Juewen Liu

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 13, 2024

Nanozymes, characterized by their nanoscale size and enzyme-like catalytic activities, exhibit diverse therapeutic potentials, including anti-oxidative, anti-inflammatory, anti-microbial, anti-angiogenic effects. These properties make them highly valuable in nanomedicine, particularly ocular therapy, bypassing the need for systemic delivery. Nanozymes show significant promise tackling multi-factored diseases, those influenced oxidation inflammation, like dry eye disease, age-related macular degeneration. Their small size, coupled with ease of modification integration into soft materials, facilitates effective penetration barriers, thereby enabling targeted or prolonged therapy within eye. This review is dedicated to exploring diseases that are intricately linked shedding light on role nanozymes managing these conditions. Additionally, recent studies elucidating advanced applications therapeutics, along materials disease management, discussed. Finally, this outlines directions future investigations aimed at bridging gap between nanozyme research clinical applications.

Language: Английский

Citations

6
Nanotechnology for vision restoration DOI
Guglielmo Lanzani, Greta Chiaravalli, Elisabetta Colombo

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 829 - 848

Published: July 18, 2024

Language: Английский

Citations

4
Axial Shortening Effects of Repeated Low-level Red-light Therapy in Children with High Myopia: A Multicenter Randomized Controlled Trial DOI Creative Commons
Guihua Liu,

Lin Liu,

Rong Hua

et al.

American Journal of Ophthalmology, Journal Year: 2024, Volume and Issue: 270, P. 203 - 215

Published: Oct. 16, 2024

To evaluate the effectiveness and safety of repeated low-level red-light (RLRL) in delaying progression high myopes with -6.00 diopters (D) or worse. Multicenter, randomized, parallel-group, single-blind clinical trial. A total 202 myopic children aged 7 to 12 years cycloplegia spherical equivalent (SE) refraction ≤-6.00 D, astigmatism less than 2.50 anisometropia 1.50 D were enrolled from March 2022 December 2022. Follow-up was completed 2023. Eligible participants randomly allocated intervention (RLRL + single vision spectacle) control group (single spectacle). The RLRL treatment administered every day for 3 minutes, twice a day, an interval at least 4 hours. primary outcome change axial length (AL) months compared baseline. Secondary outcomes included changes SE, choroidal thickness (ChT), retinal (RT) different circle sectors. Outcomes analyzed by means intention-to-treat per-protocol methods. After treatment, AL SE -0.11 ± 0.25 mm 0.18 0.63 0.32 0.09 -0.80 0.42 group, respectively. Axial shortening >0.05 59% 0% months. ChT RT center analyzed. In thickened all sectors increased parafoveal perifoveal circles. only significantly thinner multivariate linear regression model revealed significant correlations between central foveal 1 month No fundus structure changes, afterimage exceeding 6 best-corrected visual acuity decrease reported. could effectively shorten inhibit myopia patients is sustained over treatment. These observed appeared be associated increases RT.

Language: Английский

Citations

4
Regulator of oxidative balance: Research progress of nanozymes in ROS-related diseases DOI
Xiang Wang, Zidong Wang, Siyuan Hu

et al.

Materials Today Chemistry, Journal Year: 2025, Volume and Issue: 44, P. 102540 - 102540

Published: Jan. 22, 2025

Language: Английский

Citations

0
Functional Evolution of Gold Nanoclusters under Light‐Induction: From Fluorescence to Multi‐Enzyme Mimetic Transition DOI Open Access
Jing Yang,

Ruishu Xu,

Haiyan Li

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Gold nanoclusters (AuNCs) exhibit unique physicochemical properties but their functional transitions under external stimuli remain underexplored. This study reveals that ultraviolet (UV) irradiation triggers structural evolution in AuNCs, causing fluorescence loss while activating dual enzyme-mimetic activities. UV-induced particle enlargement and surface restructuring endow AuNCs with peroxidase-like (POD-like) laccase-like functionalities. The POD-like activity enables sensitive glucose detection human serum, the capability facilitates simultaneous degradation of phenolic pollutants wastewater. These light-responsive transformations demonstrate AuNCs' dynamic adaptability, bridging intrinsic catalytic properties. findings elucidate photo-regulation mechanisms nanomaterial functionality, proposing a novel strategy for designing multifunctional nanosystems applicable biosensing environmental remediation. work advances development adaptive nanomaterials programmable features biomedical ecological applications.

Language: Английский

Citations

0