Protein Purification Using Chitosan‐Modified Polyacrylonitrile Membranes DOI

Gizele G. F. Andrade,

Andressa Nathally Rocha Leal, Jeferson Gomes de Andrade

et al.

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

ABSTRACT Protein purification is essential for biomedical and industrial applications, but current methods often face challenges like low efficiency, high cost, limited selectivity. To overcome these drawbacks, we developed electrospun polyacrylonitrile (PAN) membranes with chitosan (Cs) evaluated their ability to capture bovine serum albumin (BSA) immunoglobulin G (IgG) from aqueous solutions. Comparing PAN/Cs pristine PAN membranes, showed Cs significantly enhances BSA adsorption. The were characterized using scanning electron microscopy, Fourier Transform Infrared Spectroscopy, tensile testing, surface area analysis, energy‐dispersive X‐ray spectroscopy, contact angle measurements. exhibited smooth, uniform fibers averaging 0.454 μm in diameter. maximum adsorption capacity () was 75.61 mg g −1 21.17 PAN, while IgG, 28.83 19.77 PAN. SDS‐PAGE electrophoresis confirmed that the eluted proteins maintained quality integrity. Elution efficiency of reached 89.13% 62.16% IgG. Additionally, selectively captured IgG when co‐dissolved These results position as promising materials efficient, cost‐effective, selective protein purification, potential applications both fields.

Language: Английский

Impact of Glycosylation of Apolipoprotein D on Its Interaction with Gold Nanoparticles: Insights from Molecular Dynamics Simulations DOI

Xiaolei Li,

Zengshuai Yan,

Yu‐qiang Ma

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Efficient delivery of nanoparticles (NPs) as carriers for biochemical substances is crucial in various biomedical applications. In this study, we systematically investigate the interactions between glycosylated and nonglycosylated forms Apolipoprotein D (ApoD) with gold (AuNPs) functionalized different polymer coatings, including polyethylene glycol (PEG) zwitterionic polymers. Using all-atom molecular dynamics simulations, demonstrate that glycosylation significantly enhances adsorption behavior ApoD on AuNP surfaces, extent enhancement being dependent type (especially charge property) coatings. Notably, while polymers exhibit strong resistance to protein their form, antifouling capability diminished when present. Further, our findings reveal not only strengthens binding energy proteins but also alters hydration at NP–protein interface. Overall, study provides a deeper understanding role modulating protein–nanoparticle interactions, which essential design more effective nanomaterials precision medicine.

Language: Английский

Citations

1
The nanoparticle-Protein Corona untold history (1907–2007) DOI
Eudald Casals, Michele Vitali, Víctor Puntes

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 58, P. 102435 - 102435

Published: Aug. 5, 2024

Language: Английский

Citations

6
Looking back, moving forward: protein corona of lipid nanoparticles DOI
Yue Gao,

Yeqi Huang,

Chuanyu Ren

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(23), P. 5573 - 5588

Published: Jan. 1, 2024

Intelligent delivery of lipid nanoparticles can be achieved through rational design protein corona as a “troublemaker”.

Language: Английский

Citations

4
Effect of Nanoparticle Curvature on Its Interaction with Serum Proteins DOI
Yue‐wen Yin, Yu‐qiang Ma, Hong‐ming Ding

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(29), P. 15205 - 15213

Published: July 11, 2024

The size or the curvature of nanoparticles (NPs) plays an important role in regulating composition protein corona. However, molecular mechanisms how affects interaction NPs with serum proteins still remain elusive. In this study, we employ all-atom dynamics simulations to investigate interactions between two typical and PEGylated Au three different surface curvatures (0, 0.1, 0.5 nm

Language: Английский

Citations

4
Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing DOI
Xiaotong Zhu, Xinyu Li, Li Qi

et al.

Chemical Research in Chinese Universities, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Language: Английский

Citations

0
Antiprotein Corona-Fouling Effect of the Double-Stranded DNA Coating Layer on the Gold Nanoparticles-Small Molecule Adsorbent Probe DOI

Zongming Qin,

Xiaoqi Tao, Yingxin Pang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

The formation of a protein corona (PC) can significantly impact the detection ability gold nanoparticles (AuNPs)-small molecule adsorbent probes based on competitive adsorption. To alleviate this problem, in study, double-stranded DNA (dsDNA) was introduced to modify AuNP mitigate negative effect PCs small molecules by taking AuNPs-dichlorofluorescein (DCF) probe-based ambroxol hydrochloride (AMB) as study model. It found that dsDNA-modified AuNPs-DCF probe (dsDNA@AuNPs-DCF), accuracy for AMB improved, which might be attributed isolation proteins from surface AuNPs while still allowing access due introduction rigid dsDNA. Further, strand length and number dsDNA modified antifouling performance then investigated, it LOD value artificial milk samples dsDNA10bp90@AuNPs-DCF (with 10 base 90:1 ratio AuNPs) is decreased more than 2-fold compared with probe. Moreover, probes, recovery rates analyzed commercial greatly improved probe, particularly when contained much lower concentrations. This demonstrates dsDNA-based antiprotein corona-fouling strategy AuNPs-small provides beneficial ideas dealing interference resulting studying biological samples.

Language: Английский

Citations

0
Design Principles Of Inorganic‐Protein Hybrid Materials for Biomedicine DOI Creative Commons
Hao Liu, Xiaohui Liu, Hui Jiang

et al.

Exploration, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

ABSTRACT Inorganic protein hybrid materials (IPHMs) due to editable structure present unrivalled potential at the intersection of synthetic biology and science. The synthesis IPHMs with a high degree biosafety from bioactive units represents shift in material design synthesis. This paper focuses on review structural basis principles proteins for specific physical chemical functions. It also provides valuable reference emerging through conformational relationship IPHMs, which extends applications IPHMs. In addition, construction strategy reaction system is analyzed perspective biology. possibility engineering batch discussed. Based physicochemical properties different applied‐oriented research biomedical optical imaging multimodal therapy, idea situ proposed. Ultimately, trends challenges are speculated detail.

Language: Английский

Citations

0
Color-barcode yeasts@nano-gold magnetic composite plasmonic microspheres for suspension arrays DOI
Yao Zhang, Di Ren,

Yanyan Chai

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162850 - 162850

Published: April 1, 2025

Language: Английский

Citations

0
Investigation of the Antioxidant and Cellular Toxicity Activities of Gold Nanoparticles Synthesized Using <i>Cichorium Intybus</i> Extract on a Liver Cancer Cell Line DOI Creative Commons

Mohammad Mohammad-Alizadeh,

Ahmad Asgharzadeh, Maryam Tatari

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 24(1)

Published: April 14, 2025

Background: Liver cancer is increasing in different parts of the world and fourth leading cause death globally. Objectives: The present study aims to synthesize analyze characterization gold nanoparticles (AuNPs) synthesized by Cichorium intybus extract evaluate their antioxidant cellular toxicity activity against liver cells (HepG2). Methods: AuNPs were characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), fourier-transform infrared spectroscopy (FTIR). was assessed DPPH test, cytotoxicity analyzed MTT method. Results: results indicate that are crystalline materials with a particle size less than 100 nm, mean 23.94 nm. FTIR reveals presence biochemical groups act as reducing factors. demonstrate increases concentration, 87% inhibition free radical scavenging observed at 250 µg/mL. Cell cell lines (HepG2) demonstrated significant time- dose-dependent manner. percentage viability concentration 1000 µg/ml after 24, 48, 72 hours determined be 45%, 51%, 22%, respectively. Conclusions: revealed simple cost-effective environmentally friendly method could employed from food pharmaceutical industries.

Language: Английский

Citations

0
Liquid chromatography-based metallomics and transmission electron microscopy reveal gold nanoparticle surface treatment with vicinal dithiols to abolish protein corona formation DOI

Negar Pourzadi,

Lorenzo Chiaverini,

Magdalene Patricia Gieschen

et al.

Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: April 30, 2025

While gold nanoparticles (AuNPs) should allow the delivery of surface immobilized drugs to intended target tissues via bloodstream, their interactions with plasma proteins may induce aggregation and thus impede an effective chemotherapeutic agents tissues. The deliberate treatment AuNPs has potential overcome this inherent limitation. To probe between treated in blood plasma, we employed a size-exclusion chromatography (SEC)-based metallomics tool together transmission electron microscopy (TEM). After addition citrate capped its analysis revealed >670 kDa Au species, which TEM identified as AuNP-plasma protein aggregates. ameliorate formation latter, was modified dithiothreitol (DTT), meso 2,3-dimercaptosuccinic acid (DMSA), or 2,3 dimercapto-1-propionesulfonic (DMPS) effect probed after these rabbit plasma. results for DMSA/DMPS-treated that tight binding dithiols more significantly reduced corona compared DTT-AuNPs implying DMSA DMPS is feasible strategy control AuNP-based using targeting sequences cancer can be enhanced by DMPS. Since left over AuNP mobilized iron from metalloproteins, excess must removed before injecting patients.

Language: Английский

Citations

0