Journal of Applied Polymer Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 20, 2024
ABSTRACT
Protein
purification
is
essential
for
biomedical
and
industrial
applications,
but
current
methods
often
face
challenges
like
low
efficiency,
high
cost,
limited
selectivity.
To
overcome
these
drawbacks,
we
developed
electrospun
polyacrylonitrile
(PAN)
membranes
with
chitosan
(Cs)
evaluated
their
ability
to
capture
bovine
serum
albumin
(BSA)
immunoglobulin
G
(IgG)
from
aqueous
solutions.
Comparing
PAN/Cs
pristine
PAN
membranes,
showed
Cs
significantly
enhances
BSA
adsorption.
The
were
characterized
using
scanning
electron
microscopy,
Fourier
Transform
Infrared
Spectroscopy,
tensile
testing,
surface
area
analysis,
energy‐dispersive
X‐ray
spectroscopy,
contact
angle
measurements.
exhibited
smooth,
uniform
fibers
averaging
0.454
μm
in
diameter.
maximum
adsorption
capacity
()
was
75.61
mg
g
−1
21.17
PAN,
while
IgG,
28.83
19.77
PAN.
SDS‐PAGE
electrophoresis
confirmed
that
the
eluted
proteins
maintained
quality
integrity.
Elution
efficiency
of
reached
89.13%
62.16%
IgG.
Additionally,
selectively
captured
IgG
when
co‐dissolved
These
results
position
as
promising
materials
efficient,
cost‐effective,
selective
protein
purification,
potential
applications
both
fields.
Chemistry - An Asian Journal,
Journal Year:
2024,
Volume and Issue:
19(23)
Published: Sept. 2, 2024
Abstract
The
Zika
virus
(ZIKV)
is
a
global
health
threat
due
to
its
rapid
spread
and
severe
implications,
including
congenital
abnormalities
neurological
complications.
Differentiating
ZIKV
from
other
arboviruses
such
as
dengue
(DENV)
crucial
for
effective
diagnosis
treatment.
This
study
presents
the
development
of
biosensor
detecting
non‐structural
protein
1
(NS1)
using
gold
nanoparticles
(AuNPs)
functionalized
with
monoclonal
antibodies
employing
dynamic
light
scattering
(DLS).
named
ZINS1‐mAb‐AuNP
exhibited
specific
binding
NS1
protein,
demonstrating
high
colloidal
stability
indicated
by
hydrodynamic
diameter
(D
H
)
140
nm,
detectable
via
DLS.
In
absence
ionic
strength
medium
caused
particle
aggregation.
detection
method
showed
good
sensitivity
specificity,
limit
(LOD)
0.96
μg
mL
−1
,
avoided
cross‐reactivity
DENV2
SARS‐CoV‐2
spike
proteins.
represents
promising
tool
early
accurate
ZIKV,
facilitating
diagnostic
treatment
capabilities
arboviral
infections.
Photochem,
Journal Year:
2025,
Volume and Issue:
5(1), P. 3 - 3
Published: Jan. 23, 2025
Atomically
precise
noble
metal
nanoclusters
protected
by
ligands
are
broadly
discussed
in
the
literature
as
a
promising
new
class
of
materials
with
many
interesting
properties.
Of
those,
most
prominent
is
characteristic
luminescence
visible
and
near-infrared
light.
Within
plethora
conjugates
to
various
protective
ligands,
protein-enveloped
systems
present
several
unique
features
arising
from
an
interplay
nanocluster
photophysics
protein
chemistry
along
its
macromolecular
dynamics.
The
specific
properties
protein–metal
underlie
applications
these
systems,
especially
bioimaging.
This
review,
contrast
already
published,
focuses
on
protein-protected
gold
(AuNCs)
standpoint
proteinaceous
shell
which
plays
crucial
role
biocompatibility,
solubility,
excellent
in-solution
stability
such
nanohybrid
complexes.
Factors
protein’s
size,
structural
rigidity,
amino
acid
composition,
electric
charge,
electron
donor
composite
acids
discussed.
Biomedical Materials,
Journal Year:
2024,
Volume and Issue:
19(4), P. 042010 - 042010
Published: June 5, 2024
To
improve
the
translational
and
clinical
applications
of
gold
nanoparticles
(GNPs)
in
medicine
there
is
a
need
for
better
understanding
physicochemical
properties
relation
to
systemic
parameters
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(10), P. 5539 - 5539
Published: May 19, 2024
Encapsulation
with
polymers
is
a
well-known
strategy
to
stabilize
and
functionalize
nanomaterials
tune
their
physicochemical
properties.
Amphiphilic
copolymers
are
promising
in
this
context,
but
structural
diversity
complexity
also
make
understanding
predicting
behavior
challenging.
This
particularly
the
case
complex
media
which
relevant
for
intended
applications
medicine
nanobiotechnology.
Here,
we
studied
encapsulation
of
gold
nanoparticles
quantum
dots
amphiphilic
differing
charge
molecular
structure.
Protein
adsorption
nanoconjugates
was
fluorescence
correlation
spectroscopy,
surface
activity
dynamic
interfacial
tensiometry.
without
affecting
characteristic
properties
possible
all
tested
provided
good
stabilization.
However,
interaction
proteins
cells
significantly
depended
on
details.
We
identified
statistical
providing
strongly
reduced
protein
low
unspecific
cellular
uptake.
Interestingly,
different
zwitterionic
showed
substantial
differences
resulting
bio-repulsive
Among
herein,
sulfobetaine
phosphatidylcholine
sidechains
performed
better
than
carboxylic
acid-
dimethylamino-terminated
sidechains.
Vaccines,
Journal Year:
2024,
Volume and Issue:
12(8), P. 829 - 829
Published: July 23, 2024
Gold
nanoparticles
(AuNPs)
decorated
with
antigens
have
recently
emerged
as
promising
tools
for
vaccine
development
due
to
their
innate
ability
provide
stability
and
modulate
immune
responses.
In
this
study,
we
engineered
deactivated
virus-like
particles
(VLPs)
by
precisely
functionalizing
gold
cores
coronas
comprising
the
full
SARS-CoV-2
spike
protein
(S).
Using
BALB/c
mice
a
model,
investigated
immunogenicity
of
these
S-AuNPs-VLPs.
Our
results
demonstrate
that
S-AuNPs-VLPs
consistently
enhanced
antigen-specific
antibody
responses
compared
S
free
in
solution.
This
enhancement
included
higher
binding
titers,
neutralizing
capacity
antibodies,
stronger
T-cell
Compared
mRNA
COVID-19
vaccine,
where
is
synthesized
situ,
induced
comparable
responses,
but
substantially
superior
conclusion,
our
study
highlights
potential
conjugated
AuNPs
an
effective
antigen-delivery
system
protein-based
vaccines
targeting
broad
spectrum
infectious
diseases
other
emergent
viruses.
Nanomaterials,
Journal Year:
2024,
Volume and Issue:
14(22), P. 1839 - 1839
Published: Nov. 17, 2024
Surface-enhanced
Raman
scattering
(SERS)
is
an
innovative
spectroscopic
technique
that
amplifies
the
signals
of
molecules
adsorbed
on
rough
metal
surfaces,
making
it
pivotal
for
single-molecule
detection
in
complex
biological
and
environmental
matrices.
This
review
aims
to
elucidate
design
strategies
recent
advancements
application
standalone
SERS
nanoprobes,
with
a
special
focus
quantifiable
tags.
We
conducted
comprehensive
analysis
literature,
focusing
development
nanoprobes
employ
novel
nanostructuring
techniques
enhance
signal
reliability
quantification.
Standalone
exhibit
significant
enhancements
sensitivity
specificity
due
optimized
hot
spot
generation
improved
reporter
molecule
interactions.
Recent
innovations
include
nanogap
core-satellite
structures
electromagnetic
fields,
which
are
crucial
applications.
particularly
those
utilizing
indirect
mechanisms,
represent
advancement
field.
They
hold
potential
wide-ranging
applications,
from
disease
diagnostics
monitoring,
owing
their
enhanced
ability
operate
under
sample
conditions.
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(50), P. 49283 - 49292
Published: Dec. 3, 2024
With
the
development
of
nanotechnology,
there
is
growing
interest
in
using
nanoparticles
(NPs)
for
biomedical
applications,
such
as
diagnostics,
drug
delivery,
imaging,
and
nanomedicine.
The
protein's
structural
stability
plays
a
pivotal
role
its
functionality,
any
alteration
this
structure
can
have
significant
implications,
including
disease
progression.
Herein,
we
performed
combined
experimental
computational
study
effect
gold
NPs
with
diameter
5
nm
(5
Au-NPs)
on
bovine
serum
albumin
(BSA)
protein
absence
presence
NaCl
salt.
Circular
dichroism
spectroscopy
showed
loss
secondary
BSA
due
to
synergistic
Au-NPs
NaCl,
Thioflavin
T
fluorescence
assays
suppressed
β-sheet
formation
PBS,
emphasizing
intricate
interplay
between
physiological
conditions.
Additionally,
molecular
dynamics
(MD)
simulations
revealed
that
Au-NP
induced
changes
monomer
highlighting
initial
binding
mechanism
Au-NP.
Furthermore,
MD
explored
smaller
(3
nm)
nanocluster
(Au-NC
size
1
sites
monomer.
Although
stable
BSA-Au
conjugates
was
different
sizes,
no
specific
were
observed.
Moreover,
small
size,
Au-NC
decreased
helical
content
hydrogen
bonds
monomer,
promoting
unfolding
more
significantly.
In
summary,
provides
comprehensive
insights
into
interactions
among
Au
nanosized
substances,
BSA,
conditions
are
essential
developing
tailored
nanomaterials
enhanced
biocompatibility
efficacy.
Journal of Applied Polymer Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 20, 2024
ABSTRACT
Protein
purification
is
essential
for
biomedical
and
industrial
applications,
but
current
methods
often
face
challenges
like
low
efficiency,
high
cost,
limited
selectivity.
To
overcome
these
drawbacks,
we
developed
electrospun
polyacrylonitrile
(PAN)
membranes
with
chitosan
(Cs)
evaluated
their
ability
to
capture
bovine
serum
albumin
(BSA)
immunoglobulin
G
(IgG)
from
aqueous
solutions.
Comparing
PAN/Cs
pristine
PAN
membranes,
showed
Cs
significantly
enhances
BSA
adsorption.
The
were
characterized
using
scanning
electron
microscopy,
Fourier
Transform
Infrared
Spectroscopy,
tensile
testing,
surface
area
analysis,
energy‐dispersive
X‐ray
spectroscopy,
contact
angle
measurements.
exhibited
smooth,
uniform
fibers
averaging
0.454
μm
in
diameter.
maximum
adsorption
capacity
()
was
75.61
mg
g
−1
21.17
PAN,
while
IgG,
28.83
19.77
PAN.
SDS‐PAGE
electrophoresis
confirmed
that
the
eluted
proteins
maintained
quality
integrity.
Elution
efficiency
of
reached
89.13%
62.16%
IgG.
Additionally,
selectively
captured
IgG
when
co‐dissolved
These
results
position
as
promising
materials
efficient,
cost‐effective,
selective
protein
purification,
potential
applications
both
fields.
