Multiplex Profiling of miR-122 for Preclinical and Clinical Evaluation of Drug-Induced Liver Injury by a Full-Scale Platform DOI

Wuqi Dong,

Weizhen Yan,

Yue-chen Xu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(36), P. 24860 - 24871

Published: Aug. 28, 2024

Diagnostic and monitoring for drug-induced liver injury (DILI) predominantly rely on serum aminotransferases. However, owing to their widespread expression across multiple organs, a significant challenge emerges from the absence of reliable biomarkers DILI diagnosis. Herein, we introduce concept detection, circumventing nonspecific elevation delayed release aminotransferases then straightforwardly focusing core feature DILI, abnormal gene caused by drug overdose. The developed full-scale platform integrates properties spherical nucleic acids with elaborately designed fluorescence in situ hybridization sequences, enabling sensitive specific profiling drug-overdosed miR-122 alterations molecular, cellular, organismal, clinical scales effectively bypassing phenotypic features disease. Furthermore, diagnostic efficacies total RNA extracted both mouse human blood samples diagnosis were analyzed using receiver operating characteristic curve principal component analysis. We anticipate that this universal holds potential facilitating diagnosis, therapeutic evaluation, prognosis.

Language: Английский

Evaluation of the Multidimensional Enhanced Lateral Flow Immunoassay in Point-of-Care Nanosensors DOI
Sijie Liu,

Yangjun Liao,

Rui Shu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Point-of-care (POC) nanosensors with high screening efficiency show promise for user-friendly manipulation in the ever-increasing on-site analysis demand illness diagnosis, environmental monitoring, and food safety. Currently, inspired by merits of integrating advanced nanomaterials, molecular biology, machine learning, artificial intelligence, lateral flow immunoassay (LFIA)-based POC have been devoted to satisfying commercial demands terms sensitivity, specificity, practicality. Herein, we examine use multidimensional enhanced LFIA various fields over past two decades, focusing on introducing nanomaterials improve acquisition capability small order magnitude targets through engineering transformations emphasizing interdomain fusion collaboratively address inherent challenges current applications, such as multiplexing, development detectors quantitative analysis, more practical sensitivity enhancement. Specifically, this comprehensive review encompasses latest advances comprehending an alternative signal transduction pattern, aiming achieve rapid, ultrasensitive, "sample-to-answer" available options progressive applications nanosensors. In summary, cross-collaboration disciplines, has potential break barriers toward commercialization laboratory-level nanosensors, thus leading emergence next generation LFIA.

Language: Английский

Citations

23
Recent advances in low‐background luminescent nanomaterials‐enhanced lateral flow assays for clinical diagnosis DOI Creative Commons

Liangwen Hao,

Jing Chen,

Quanshuang Li

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Lateral flow assays (LFAs) are widely applicable in clinical point‐of‐care testing (POCT) due to their unique advantages such as simplicity, rapidity, and cost‐effectiveness. However, sensitivity is often constrained by the background autofluorescence of biological sample, self‐matrix, or readout technique, thereby leading overlooking trace amounts biomarkers present early disease. In recent years, various nanomaterials‐based methods have been developed address this issue, including time‐gated wavelength‐differentiated strategy external modulated for separation minimize interference from samples. This review provides a comprehensive overview low‐background luminescent nanoparticles (LBLNPs)‐enhanced LFA systems, focusing on analyzing underlying mechanism these nanomaterials improving accuracy LFAs platform diagnosis. Representative examples selected demonstrate potential detecting disease‐associated samples blood, urine, saliva et al. Finally, unresolved challenges future development prospects briefly discussed.

Language: Английский

Citations

1
A Fully Integrated ICP‐Enriched and Nanozyme‐Catalyzed Lateral Flow Assay for cfDNA Detection in Whole Blood DOI Open Access

Fei Cun,

Hanxue Li, Haonan Wang

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Rapid and sensitive detection of Epstein−Barr virus cell‐free DNA (EBV cfDNA) is crucial for early diagnosis monitoring nasopharyngeal carcinoma (NPC), but accessibility to screening limited by complicated costly conventional isolation purification approaches. Here, a fully integrated ion concentration polarization (ICP)‐enriched nanozyme‐catalyzed lateral flow assay (ICP‐cLFA) developed, enabling total analysis EBV cfDNA in whole blood samples, with isolation, pre‐concentration, amplification performed on microfluidic chip, consequently providing the signal readout within 75 min. Specifically, ICP preconcentration steps, together target recognition catalyzed platinum‐decorated mesoporous gold nanosphere (MGNS@Pt) nanozyme, result an ultralow limit 4 aM standard samples 100 from NPC‐bearing rats. The high sensitivity specificity ICP‐cLFA suggest strong potential resource‐limited clinical field applications.

Language: Английский

Citations

1
Ultrasensitive Electrochemiluminescence Biosensor Based on DNA-Bio-Bar-Code and Hybridization Chain Reaction Dual Signal Amplification for Exosomes Detection DOI
Xiangdan Meng,

Xuejiao Pang,

Xiangyu Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(32), P. 13299 - 13307

Published: Aug. 2, 2024

Exosomes have received considerable attention as potent reference markers for the diagnosis of various neoplasms due to their close and direct relationship with proliferation, adhesion, migration tumor. The ultrasensitive detection cancer-derived low-abundance exosomes is imperative, but still a great challenge. Herein, we report an electrochemiluminescence (ECL) biosensor based on DNA-bio-bar-code hybridization chain reaction (HCR)-mediated dual signal amplification exosomes. In this system, two types aptamers were modified magnetic nanoprobe (MNPs) gold nanoparticles (AuNPs) numerous bio-bar-code DNA, respectively, which formed "sandwich" structures in presence specific target separated under field, DNA released by dissolving AuNPs. triggered HCR procedure produce good deal long duplex structure embedding hemin, generated strong ECL coreactors Under optimal conditions, it exhibited linearly ranging from 10

Language: Английский

Citations

5
Integrated Ultrasound‐Enrichment and Machine Learning in Colorimetric Lateral Flow Assay for Accurate and Sensitive Clinical Alzheimer's Biomarker Diagnosis DOI Creative Commons

Shu‐Qing Wang,

Yan Zhu,

Zhongzeng Zhou

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(42)

Published: Sept. 19, 2024

Abstract The colloidal gold nanoparticle (AuNP)‐based colorimetric lateral flow assay (LFA) is one of the most promising analytical tools for point‐of‐care disease diagnosis. However, low sensitivity and insufficient accuracy still limit its clinical application. In this work, a machine learning (ML)‐optimized LFA with ultrasound enrichment developed to achieve sensitive accurate detection tau proteins early screening Alzheimer's (AD). device integrated portable ultrasonic actuator rapidly enrich microparticles using ultrasound, which essential sample pre‐enrichment improve sensitivity, followed by ML algorithms classify predict enhanced signals. results undiluted serum testing show that protocol enables efficient classification quantification AD biomarker protein concentration an average 98.11% prediction 99.99%, achieving (LOD) as 10.30 pg mL −1 . Further (POCT) human plasma samples demonstrates potential use in trials. Such reliable immunosensor high precision superb sensing performance expected put perspective diagnostic platform.

Language: Английский

Citations

5
Supramolecular DNA Nanodevice Assembled via RCA and HCR Cascade Reaction on Tetrahedral DNA Nanostructure for Sensitive Detection and Intracellular Imaging of Dual-miRNAs Associated with Liver Cancer DOI

Xue-Mei Zhou,

Si–Yi Chen, Yaqin Chai

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade on tetrahedral nanostructure (TDN) to achieve simultaneous sensitive detection intracellular imaging of dual-miRNAs related liver cancer. The effectively addressed limitations low probe loading capacity in traditional TDN nanodevices by enriching plenty signal probes around single TDN, significantly enhancing fluorescence signal. Impressively, with fulcrum dense structure imparted strong rigidity, ensuring stability decrease aggregation quenching for further increasing response. Consequently, biosensor based enabled miRNA221 miRNA222, achieved accurate situ displaying improved capabilities compared TDN-based nanodevices. More importantly, precise miRNA222 could distinguish hepatocellular carcinoma cells different degrees metastasis from human normal cells, providing more information diagnosis development carcinoma.

Language: Английский

Citations

0
Simultaneous or separate detection of heavy metal ions Hg2+ and Ag+ based on lateral flow assays DOI
Zhili Wang,

Yufang Cheng,

Ting Tang

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(2)

Published: Jan. 21, 2025

Language: Английский

Citations

0
Cu2+-encapsulated DNA nanosphere and filter enable sensitive and rapid analysis of miRNA-155 DOI
Weijing Liu, Yue Wang, Peng Wu

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 274, P. 117203 - 117203

Published: Jan. 28, 2025

Language: Английский

Citations

0
Signal-amplifying biosensors for miRNA detection and their extension in portable glucometer DOI

Xiaofang Liu,

Qun Wang, Huan Liu

et al.

Talanta, Journal Year: 2025, Volume and Issue: 288, P. 127714 - 127714

Published: Feb. 13, 2025

Language: Английский

Citations

0
Nanoscience in Action: Unveiling Emerging Trends in Materials and Applications DOI Creative Commons
Kevin J. Hughes,

Magesh Ganesan,

Rumiana Koynova

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Since their inception in the early 1960s, use of nanoscale materials has progressed leaps and bounds, role diverse fields ranging from human health to energy is undeniable. In this report, we utilize CAS Content Collection, a vast repository scientific information extracted journals patent publications, identify emerging topics field. This involves understanding trends, such as growth certain over time, well establishing relationships among topics. We achieved by using host strategies including quantitative natural language processing (NLP) approach 270 subtopics across three major categories-materials, applications, properties-by surveying 3 million documents spanning two decades nanoscience landscape. wealth been condensed into several conceptual Trendscape maps other data visualizations, providing metrics related identified concepts, grouped hierarchical classes, connections between them have explored. Our extensive analysis taking advantage an NLP-based along with robust indexing provides valuable insights field that hope can help inform drive future research efforts. series interconnected papers, will present our findings project, focus on four applications materials-drug delivery, sensors, energy, catalysis-to provide more comprehensive detailed picture nanotechnology these fields.

Language: Английский

Citations

0