Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy DOI
Junyi Zeng, Xinbo Huang,

Yajie Yang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 701 - 710

Published: Dec. 16, 2024

Controlling CRISPR/Cas9 gene editing at the spatiotemporal resolution level, especially for in vivo applications, remains a great challenge. Here, we developed near-infrared (NIR) light-activated nanophotonic system (UCPP) controlled CRISPR-Cas9 and synergistic photodynamic therapy (PDT). Lanthanide-doped upconversion nanoparticles are not only employed as carriers intracellular plasmid delivery but also serve nanotransducers to convert NIR light (980 nm) into visible with emission 460 650 nm, which could result simultaneous activation of PDT processes, respectively. Such unique design achieves light-controlled precise hypoxia-inducible factor 1α minimal off-target effect, effectively ameliorates hypoxic state tumor sites, facilitates deep-seated process antitumor effect. This optogenetically activatable nanosystem holds potential spatially targeted cancer therapy.

Language: Английский

Advances and applications of peripheral optogenetics in animal models DOI

W. Zhou,

Liping Jia, Lupeng Yue

et al.

Neuroscience, Journal Year: 2025, Volume and Issue: 567, P. 163 - 171

Published: Jan. 5, 2025

Language: Английский

Citations

0
Neural circuit mechanisms of epilepsy: Maintenance of homeostasis at the cellular, synaptic, and neurotransmitter levels DOI Creative Commons

Xueqing Du,

Yi Wang, Xuefeng Wang

et al.

Neural Regeneration Research, Journal Year: 2025, Volume and Issue: 21(2), P. 455 - 465

Published: Jan. 13, 2025

Epilepsy, a common neurological disorder, is characterized by recurrent seizures that can lead to cognitive, psychological, and neurobiological consequences. The pathogenesis of epilepsy involves neuronal dysfunction at the molecular, cellular, neural circuit levels. Abnormal molecular signaling pathways or specific cell types disrupting normal functioning circuits. continuous emergence new technologies rapid advancement existing ones have facilitated discovery comprehensive understanding mechanisms underlying epilepsy. Therefore, this review aims investigate current in based on various technologies, including electroencephalography, magnetic resonance imaging, optogenetics, chemogenetics, deep brain stimulation, brain–computer interfaces. Additionally, discusses these from three perspectives: structural, synaptic, transmitter findings reveal encompass information transmission among different structures, interactions within same structure, maintenance homeostasis neurotransmitter These offer insights for investigating pathophysiological enhancing its clinical diagnosis treatment.

Language: Английский

Citations

0
Size and Structural Control of Mechanoluminescent ZnS:Mn2+ Nanocrystals for Optogenetic Neuromodulation DOI
Zhongxiang Wang,

Lu Jin,

Elizaveta Tiukalova

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Mechanoluminescent materials hold immense potential for various transformative applications, from medical imaging and diagnostics to health monitoring wearable displays. Conventionally produced as bulk powders or microparticles, they face significant size limitations advanced particularly in biological systems microscale devices. This work presents an approach ZnS:Mn2+ nanocrystal synthesis that involves self-assembly subsequent calcination. In addition effective control within the nanoscale, this promotes formation of abundant stacking faults, significantly enhancing piezoelectric mechanoluminescent properties by increasing trap density reducing depth. Unlike using conventional methods, these nanocrystals demonstrate strong mechanoluminescence without requiring UV pre-excitation, light emission persists even after mechanical stress is removed. These advantageous make them promising candidates optogenetic neuromodulation, can effectively trigger electrical signals neurons upon ultrasound stimulation both with pre-excitation. The persistent prolongs duration neuronal activity, providing extended temporal window neuromodulation compared materials. study provides a scalable method producing efficient nanoparticles reveals crucial role particle defect structures determining their behavior.

Language: Английский

Citations

0
Genetics‐Based Targeting Strategies for Precise Neuromodulation DOI Creative Commons

Yuyuan He,

Zhidong Wei, Jianda Xu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Abstract Genetics‐based neuromodulation schemes are capable of selectively manipulating the activity defined cell populations with high temporal–spatial resolution, providing unprecedented opportunities for probing cellular biological mechanisms, resolving neuronal projection pathways, mapping neural profiles, and precisely treating neurological psychiatric disorders. Multimodal implementation schemes, which involve use exogenous stimuli such as light, heat, mechanical force, chemicals, electricity, magnetic stimulation in combination specific genetically engineered effectors, greatly expand their application space scenarios. In particular, advanced wireless have enabled low‐invasive targeted through local delivery navigable micro‐ nanosized stimulators. this review, fundamental principles protocols genetics‐based precision first introduced.The systematically summarized, including optical, thermal, chemical, electrical, stimulation, an emphasis on those strategies. Representative studies dissected analyzed advantages disadvantages. Finally, significance is emphasized open challenges future perspectives concluded.

Language: Английский

Citations

0
Vacancy elimination enables scaffold with amplified photo-thermal-electric effect to promote osteoblast differentiation DOI
Huixing Li, Fangwei Qi, Jian Xiong

et al.

Materials Today Chemistry, Journal Year: 2025, Volume and Issue: 47, P. 102807 - 102807

Published: June 5, 2025

Language: Английский

Citations

0
Bi Cluster Engineering for Tunable Broadband NIR Optical Response DOI

Jingfei Chen,

Quan Dong,

Ziang Liu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Abstract Near‐infrared (NIR) broadband active photonic materials play a crucial role in various domains such as optical communication, phototherapy, and medical imaging. However, the construction of NIR with tunable response still remains great challenge. This manuscript proposes topological structure engineering strategy Bi‐activated glass for realization controllable emission. By manipulating glass, Bi cluster configuration can be precisely control, size from 2.78 to 1.64 nm. Correspondingly, properties including emission, excited state absorption (ESA), even unsaturable loss (UL) rationally switched. Based on above findings, cluster‐activated bulk glasses, fibers, derived devices notably improved performance successfully fabricated. Their application potentials communication imaging are also demonstrated. The study highlights promise advancing devices. Furthermore, topological‐mediated regulation may provide new insights into fundamental science cutting technology other materials.

Language: Английский

Citations

2
Wireless activation of dopamine neurons for rapid regulation of depression-related behaviors by upconversion optogenetics DOI

Yaru Sun,

Zhengbing Liang,

Da-hai Hu

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 61, P. 102587 - 102587

Published: Dec. 7, 2024

Language: Английский

Citations

1
Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy DOI
Junyi Zeng, Xinbo Huang,

Yajie Yang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 701 - 710

Published: Dec. 16, 2024

Controlling CRISPR/Cas9 gene editing at the spatiotemporal resolution level, especially for in vivo applications, remains a great challenge. Here, we developed near-infrared (NIR) light-activated nanophotonic system (UCPP) controlled CRISPR-Cas9 and synergistic photodynamic therapy (PDT). Lanthanide-doped upconversion nanoparticles are not only employed as carriers intracellular plasmid delivery but also serve nanotransducers to convert NIR light (980 nm) into visible with emission 460 650 nm, which could result simultaneous activation of PDT processes, respectively. Such unique design achieves light-controlled precise hypoxia-inducible factor 1α minimal off-target effect, effectively ameliorates hypoxic state tumor sites, facilitates deep-seated process antitumor effect. This optogenetically activatable nanosystem holds potential spatially targeted cancer therapy.

Language: Английский

Citations

1