Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy DOI
Junyi Zeng, Xinbo Huang,

Yajie Yang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 701 - 710

Published: Dec. 16, 2024

Controlling CRISPR/Cas9 gene editing at the spatiotemporal resolution level, especially for in vivo applications, remains a great challenge. Here, we developed near-infrared (NIR) light-activated nanophotonic system (UCPP) controlled CRISPR-Cas9 and synergistic photodynamic therapy (PDT). Lanthanide-doped upconversion nanoparticles are not only employed as carriers intracellular plasmid delivery but also serve nanotransducers to convert NIR light (980 nm) into visible with emission 460 650 nm, which could result simultaneous activation of PDT processes, respectively. Such unique design achieves light-controlled precise hypoxia-inducible factor 1α minimal off-target effect, effectively ameliorates hypoxic state tumor sites, facilitates deep-seated process antitumor effect. This optogenetically activatable nanosystem holds potential spatially targeted cancer therapy.

Language: Английский

Advances and applications of peripheral optogenetics in animal models DOI

W. Zhou,

Liping Jia, Lupeng Yue

et al.

Neuroscience, Journal Year: 2025, Volume and Issue: 567, P. 163 - 171

Published: Jan. 5, 2025

Language: Английский

Citations

0
Neural circuit mechanisms of epilepsy: Maintenance of homeostasis at the cellular, synaptic, and neurotransmitter levels DOI Creative Commons

Xueqing Du,

Yi Wang, Xuefeng Wang

et al.

Neural Regeneration Research, Journal Year: 2025, Volume and Issue: 21(2), P. 455 - 465

Published: Jan. 13, 2025

Epilepsy, a common neurological disorder, is characterized by recurrent seizures that can lead to cognitive, psychological, and neurobiological consequences. The pathogenesis of epilepsy involves neuronal dysfunction at the molecular, cellular, neural circuit levels. Abnormal molecular signaling pathways or specific cell types disrupting normal functioning circuits. continuous emergence new technologies rapid advancement existing ones have facilitated discovery comprehensive understanding mechanisms underlying epilepsy. Therefore, this review aims investigate current in based on various technologies, including electroencephalography, magnetic resonance imaging, optogenetics, chemogenetics, deep brain stimulation, brain–computer interfaces. Additionally, discusses these from three perspectives: structural, synaptic, transmitter findings reveal encompass information transmission among different structures, interactions within same structure, maintenance homeostasis neurotransmitter These offer insights for investigating pathophysiological enhancing its clinical diagnosis treatment.

Language: Английский

Citations

0
Bi Cluster Engineering for Tunable Broadband NIR Optical Response DOI

Jingfei Chen,

Quan Dong,

Ziang Liu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Abstract Near‐infrared (NIR) broadband active photonic materials play a crucial role in various domains such as optical communication, phototherapy, and medical imaging. However, the construction of NIR with tunable response still remains great challenge. This manuscript proposes topological structure engineering strategy Bi‐activated glass for realization controllable emission. By manipulating glass, Bi cluster configuration can be precisely control, size from 2.78 to 1.64 nm. Correspondingly, properties including emission, excited state absorption (ESA), even unsaturable loss (UL) rationally switched. Based on above findings, cluster‐activated bulk glasses, fibers, derived devices notably improved performance successfully fabricated. Their application potentials communication imaging are also demonstrated. The study highlights promise advancing devices. Furthermore, topological‐mediated regulation may provide new insights into fundamental science cutting technology other materials.

Language: Английский

Citations

2
Wireless activation of dopamine neurons for rapid regulation of depression-related behaviors by upconversion optogenetics DOI

Yaru Sun,

Zhengbing Liang,

Da-hai Hu

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 61, P. 102587 - 102587

Published: Dec. 7, 2024

Language: Английский

Citations

1
Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy DOI
Junyi Zeng, Xinbo Huang,

Yajie Yang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 701 - 710

Published: Dec. 16, 2024

Controlling CRISPR/Cas9 gene editing at the spatiotemporal resolution level, especially for in vivo applications, remains a great challenge. Here, we developed near-infrared (NIR) light-activated nanophotonic system (UCPP) controlled CRISPR-Cas9 and synergistic photodynamic therapy (PDT). Lanthanide-doped upconversion nanoparticles are not only employed as carriers intracellular plasmid delivery but also serve nanotransducers to convert NIR light (980 nm) into visible with emission 460 650 nm, which could result simultaneous activation of PDT processes, respectively. Such unique design achieves light-controlled precise hypoxia-inducible factor 1α minimal off-target effect, effectively ameliorates hypoxic state tumor sites, facilitates deep-seated process antitumor effect. This optogenetically activatable nanosystem holds potential spatially targeted cancer therapy.

Language: Английский

Citations

1