Organ-on-a-Chip Applications in Microfluidic Platforms

Micromachines, Journal Year: 2025, Volume and Issue: 16(2), P. 201 - 201

Published: Feb. 10, 2025

Microfluidic technology plays a crucial role in organ-on-a-chip (OoC) systems by replicating human physiological processes and disease states, significantly advancing biomedical research drug discovery. This article reviews the design fabrication of microfluidic devices. It also explores how these technologies are integrated into OoC platforms to simulate environments, highlighting key principles, technological advances, diverse applications. Through case studies involving simulation multiple organs such as heart, liver, lungs, evaluates impact systems' on screening, toxicity assessment, personalized medicine. In addition, this considers technical challenges, ethical issues, future directions, looks ahead further optimizing functionality biomimetic precision OoCs through innovation, emphasizing its critical promoting medicine treatment strategies.

Language: Английский

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Bacteriorhodopsin-Based pH Sensor for Cell Culture Condition Regulation

Materials, Journal Year: 2025, Volume and Issue: 18(3), P. 478 - 478

Published: Jan. 21, 2025

In cell culture research and biotechnology, precise pH monitoring is crucial for maintaining cellular health ensuring reliable experimental outcomes. Traditional measurement methods, such as glass electrodes chemical indicators, are often limited by issues fragility, calibration requirements, potential cytotoxicity. This study presents a novel sensor based on bacteriorhodopsin (bR), light-sensitive protein that undergoes conformational changes in response to fluctuations, generating measurable photoelectric signal. The integrated bR-based electrochemical electrode flexible biosensor demonstrated, with measurements spanning the physiological range of 6.0–8.5. shows high correlation (R2 = 0.977) between photo-generated current signals pH, indicating robust performance real-time, non-invasive monitoring. biocompatibility nature this make it particularly suitable continuous environments. sensor’s practical application validated its integration into well plates tracking during growth, providing valuable insights metabolic processes growth conditions. future, efforts will focus enhancing sensitivity, stability, multi-parameter systems more comprehensive analysis.

Language: Английский

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Aptamer and Oligonucleotide-Based Biosensors for Health Applications

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 277 - 277

Published: April 29, 2025

Aptamers have emerged as powerful molecular recognition elements for biosensing applications, offering high specificity, stability, and adaptability. This review explores key considerations in designing aptamer-based sensors (aptasensors), with a focus on biomarker selection, aptamer design, detection immobilization strategies. However, challenges such biofluid stability reversibility must be addressed to improve biosensor performance. In this study, the potential of platforms diagnostics is explored, emphasizing their advantages future applications. Looking ahead, advances multifunctional aptamers, integration nanomaterials, computational optimization are highlighted promising directions enhancing effectiveness biosensing.

Language: Английский

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Nutritionally physiological cell culture medium and 3D culture influence breast tumour proteomics and anti-cancer drug effectiveness

Pharmacological Research, Journal Year: 2024, Volume and Issue: unknown, P. 107519 - 107519

Published: Nov. 1, 2024

Language: Английский

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