Construction of Triphenylamine-Based Aggregation-Induced Emission Luminogens for Lysosomes Imaging and Its Application in the Photodynamic Therapy of Cancer Cells DOI Creative Commons

Zhanguo Sun,

Bin Liu, Huijun Liu

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(11), P. 2272 - 2272

Published: May 22, 2025

Lysosomes are important acidic subcellular organelles whose dysfunction can lead to some related diseases. The development of new lysosome-imaging-guided AIEgens for the photodynamic therapy cancer cells is important. In this work, two novel organic compounds with AIE characteristics, namely, TPAB-CF3 and TPAB-diCF3, were designed synthesized by introducing weakly basic morpholinyl moiety lysosome-targeting ability into a triphenylamine-based luminogen. distorted spatial feature TPA D1-D2-π-A structure these prevented aggregation-caused quenching traditional fluorescent molecules efficiently promoted separation HOMO LUMO. outcomes features narrow single-triplet energy gap. Furthermore, TPAB-diCF3 showed bright yellow fluorescence emission peaks at 577 601 nm; large Stokes shifts 234 256 nm, respectively; excellent lysosome-targeted imaging HeLa (Pearson’s coefficient = 0.90). addition good 1O2-generation under light irradiation, achieved high-efficiency lysosome imaging-guided killing white-light irradiation.

Language: Английский

Endoplasmic Reticulum-Targeted Phototherapy Remodels the Tumor Immunopeptidome to Enhance Immunogenic Cell Death and Adaptive Anti-Tumor Immunity DOI Creative Commons
Weidong Xiao,

Mingquan Gao,

Banghui Mo

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 491 - 491

Published: March 28, 2025

Background: Endoplasmic reticulum (ER)-targeted phototherapy has emerged as a promising approach to amplify ER stress, induce immunogenic cell death (ICD), and enhance anti-tumor immunity. However, its impact on the antigenicity of dying tumor cells remains poorly understood. Methods: Laser activation ER-targeted photosensitizer ER-Cy-poNO2 was performed investigate effects antigenicity. Transcriptomic analysis carried out assess gene expression changes. Immunopeptidomics profiling used identify high-affinity major histocompatibility complex class I (MHC-I) ligands. In vitro functional studies were conducted evaluate dendritic maturation T lymphocyte activation, while in vivo experiments by combining identified peptide with poly IC Results: significantly remodeled antigenic landscape 4T-1 cells, enhancing their immunogenicity. revealed upregulation antigen processing presentation pathways. multiple MHC-I ligands, IF4G3986–994 (QGPKTIEQI) showing exceptional vitro, promoted enhanced lymphocytes activation. vivo, combination elicited robust immunity, characterized increased CD8+ infiltration, reduced regulatory (Tregs) microenvironment, elevated systemic Interferon-gamma (IFN-γ) levels, significant growth inhibition without toxicity. Conclusions: These findings establish mechanistic link between stress-driven ICD, immunopeptidome remodeling, adaptive immune highlighting potential platform for identifying peptides advancing peptide-based cancer vaccines.

Language: Английский

Citations

1
A novel imidazolium functionalized tetraphenylethane for ATP detection and photoinduced ROS generation DOI
Shengqiang Xiao, Agen Qiu, Zhenwen Zhang

et al.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112720 - 112720

Published: Feb. 1, 2025

Language: Английский

Citations

0
Optical Microneedle-Enhanced Transdermal Light Scattering for In Situ Photothermal Therapy Targeting Basal-Layer Psoriasis DOI

Ze Qiang Zhao,

S.-L. Zhang,

Ruixing Yu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Near-infrared (NIR) light-mediated photothermal and photodynamic therapies are promising for nonsurgical treatment of skin diseases. However, the skin's inherent light absorption, especially from melanin in epidermis, attenuates NIR energy penetration, limiting efficacy potentially causing off-target tissue damage. In this context, we developed subcutaneous response-enhanced microneedles (SLE MNs) that allow basal layer-localized seeding therapeutics leverage physical channels to efficiently transmit transdermally, facilitating situ scattered activation enhanced therapy outcomes. Such ultraoptical SLE MNs facilitated achieving up 80% initial power at 500 μm subcutaneously, representing an approximate 160% increase compared control groups. Additionally, conceptualized a two-segmented MN structure integrating light-guiding with photoresponsive enable precise basal-layer treatment, effectively mitigating local hyperenergy on surface attenuation within tissues. This optical patch offers transformative platform transdermal significant clinical potential.

Language: Английский

Citations

0
Photosensitive materials armed bacteriophages: Latest advances and prospects DOI
Jinyou Xu,

Zhengfang Luo,

Xiaoyu Xu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162429 - 162429

Published: April 1, 2025

Language: Английский

Citations

0
Data mining analysis of Dunhuang ancient medical prescriptions for the treatment of surgical diseases DOI Creative Commons
Ning Shen, Lei Fan

Asian Journal of Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Construction of Triphenylamine-Based Aggregation-Induced Emission Luminogens for Lysosomes Imaging and Its Application in the Photodynamic Therapy of Cancer Cells DOI Creative Commons

Zhanguo Sun,

Bin Liu, Huijun Liu

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(11), P. 2272 - 2272

Published: May 22, 2025

Lysosomes are important acidic subcellular organelles whose dysfunction can lead to some related diseases. The development of new lysosome-imaging-guided AIEgens for the photodynamic therapy cancer cells is important. In this work, two novel organic compounds with AIE characteristics, namely, TPAB-CF3 and TPAB-diCF3, were designed synthesized by introducing weakly basic morpholinyl moiety lysosome-targeting ability into a triphenylamine-based luminogen. distorted spatial feature TPA D1-D2-π-A structure these prevented aggregation-caused quenching traditional fluorescent molecules efficiently promoted separation HOMO LUMO. outcomes features narrow single-triplet energy gap. Furthermore, TPAB-diCF3 showed bright yellow fluorescence emission peaks at 577 601 nm; large Stokes shifts 234 256 nm, respectively; excellent lysosome-targeted imaging HeLa (Pearson’s coefficient = 0.90). addition good 1O2-generation under light irradiation, achieved high-efficiency lysosome imaging-guided killing white-light irradiation.

Language: Английский

Citations

0