Size and Charge Adaptive Clustered Nanoparticles Targeting the Biofilm Microenvironment for Chronic Lung Infection Management

ACS Nano, Journal Year: 2020, Volume and Issue: 14(5), P. 5686 - 5699

Published: April 22, 2020

Chronic lung infection caused by bacterial biofilms is an extremely serious clinical problem, which can lead to the failure of antibiotic therapy. Although nanoparticles have shown great potential in treatment biofilms, efficient penetration and retention still a big challenge. To address this issue, we herein fabricate size charge adaptive azithromycin (AZM)-conjugated clustered (denoted as AZM-DA NPs) therapeutic agents for treating biofilms. The NPs are prepared electrostatic complexation between AZM conjugated amino-ended poly(amidoamine) dendrimer (PAMAM) 2,3-dimethyl maleic anhydride (DA) modified poly(ethylene glycol)-block-polylysine (PEG-b-PLys). It noteworthy that disassemble acidic biofilm microenvironment (pH 6.0), leading release secondary AZM-conjugated PAMAM (PAMAM-AZM NPs). PAMAM-AZM with small positive beneficial improved inside enhanced permeabilization membrane, increased internalization AZM, thus exhibiting excellent antibiofilm activities. also favorable long-term antibacterial due reduced occurrence drug resistance. In vivo performance confirmed burden alleviated inflammation chronic model. This research not only develops innovative strategy delivery but provides effective way management biofilm-associated infections, including infection.

Language: Английский

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Biofilm‐Sensitive Photodynamic Nanoparticles for Enhanced Penetration and Antibacterial Efficiency

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 31(33)

Published: June 16, 2021

Abstract Efficient antimicrobials are urgently needed for the treatment of bacterial biofilms due to their resistance traditional drugs. Photodynamic therapy (PDT) is a new strategy that has been used combat bacteria and biofilms. Cationic photosensitizers, particularly cationic photodynamic nanoagents, usually chosen enhance antimicrobial activity. However, positively charged nanoparticles (NPs) beneficial cellular internalization, which causes increased cell cytotoxicity. Herein, pH‐sensitive nanosystem designed. Rose Bengal (RB) polydopamine (PDA) NPs decorated in layer‐by‐layer fashion with polymyxin B (PMB) gluconic acid (GA) generate functionally adaptive (RB@PMB@GA NPs). RB@PMB@GA remain negative at physiological pH exhibit good biocompatibility. When exposed an acidic infectious environment, surface charge is, turn, as result electrostatic interactions. This conversion allows effectively bind surfaces photoinactivation efficiency against gram‐negative bacteria. Most importantly, biofilm penetration eradication under conditions. Furthermore, efficiently eliminate infections vivo. study provides promising safely treating biofilm‐associated

Language: Английский

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Copper Doped Carbon Dots for Addressing Bacterial Biofilm Formation, Wound Infection, and Tooth Staining

ACS Nano, Journal Year: 2022, Volume and Issue: 16(6), P. 9479 - 9497

Published: June 17, 2022

Oral infectious diseases and tooth staining, the main challenges of dental healthcare, are inextricably linked to microbial colonization formation pathogenic biofilms. However, dentistry has so far still lacked simple, safe, universal prophylactic options therapy. Here, we report copper-doped carbon dots (Cu-CDs) that display enhanced catalytic (catalase-like, peroxidase-like) activity in oral environment for inhibiting initial bacteria (Streptococcus mutans) adhesion subsequent biofilm eradication without impacting surrounding tissues via oxygen (O2) reactive species (ROS) generation. Especially, Cu-CDs exhibit strong affinity lipopolysaccharides (LPS) peptidoglycans (PGN), thus conferring them with excellent antibacterial ability against Gram-positive (Staphylococcus aureus) Gram-negative (Escherichia coli), such they can prevent wound purulent infection promoting rapid healing. Additionally, Cu-CDs/H2O2 system shows a better performance whitening, compared results obtained other alternatives, e.g., CDs clinically used H2O2, particularly its negligible enamel dentin destruction. It is anticipated biocompatible presented this work promising nano-mouthwash eliminating biofilms, prompting healing as well highlighting their significance health management.

Language: Английский

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Photodynamic therapy: Innovative approaches for antibacterial and anticancer treatments

Medicinal Research Reviews, Journal Year: 2023, Volume and Issue: 43(4), P. 717 - 774

Published: Feb. 9, 2023

Abstract Photodynamic therapy is an alternative treatment mainly for cancer but also bacterial infections. This dates back to 1900 when a German medical school graduate Oscar Raab found photodynamic effect while doing research his doctoral dissertation with Professor Hermann von Tappeiner. Unexpectedly, revealed that the toxicity of acridine on paramecium depends intensity light in laboratory. therefore based administration photosensitizer subsequent irradiation within absorption maxima this substance followed by reactive oxygen species formation and finally cell death. Although not novelty, there endeavor various modifications therapy. For example, selectivity efficiency photosensitizer, as well types sources are still being modified improve final results The main aim review summarize anticancer antibacterial modifications, namely compounds, approaches, techniques, enhance effectiveness

Language: Английский

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Nanoparticle-based medicines in clinical cancer therapy

Nano Today, Journal Year: 2022, Volume and Issue: 45, P. 101512 - 101512

Published: June 4, 2022

Language: Английский

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Influence of filler characteristics on the performance of dental composites: A comprehensive review

Ceramics International, Journal Year: 2022, Volume and Issue: 48(19), P. 27280 - 27294

Published: July 16, 2022

Language: Английский

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Bacterial biofilm infections, their resistance to antibiotics therapy and current treatment strategies

Biomedical Materials, Journal Year: 2022, Volume and Issue: 17(2), P. 022003 - 022003

Published: Feb. 2, 2022

Nearly 80% of human chronic infections are caused due to bacterial biofilm formation. This is the most leading cause for failure medical implants resulting in high morbidity and mortality. In addition, biofilms also known serious problems food industry. Biofilm impart enhanced antibiotic resistance become recalcitrant host immune responses persistent recurrent infections. It makes clinical treatment very difficult. Reduced penetration molecules through EPS, mutation target site, accumulation degrading enzymes, expression efflux pump genes probable causes antibiotics resistance. Accordingly, strategies like administration topical combined therapy with antimicrobial peptides considered alternate options overcome A number other remediation both inhibition dispersion established have been developed. The metallic nanoparticles (NPs) their oxides recently gained a tremendous thrust as antibiofilm unique features. present comprehensive review gives understanding mechanisms provides an overview various currently available strategies, focusing primarily on applications NPs oxides.

Language: Английский

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Sustainable Biodegradable Biopolymer-Based Nanoparticles for Healthcare Applications

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3188 - 3188

Published: Feb. 6, 2023

Biopolymeric nanoparticles are gaining importance as nanocarriers for various biomedical applications, enabling long-term and controlled release at the target site. Since they promising delivery systems therapeutic agents offer advantageous properties such biodegradability, biocompatibility, non-toxicity, stability compared to toxic metal nanoparticles, we decided provide an overview on this topic. Therefore, review focuses use of biopolymeric animal, plant, algal, fungal, bacterial origin a sustainable material potential drug systems. A particular focus is encapsulation many different categorized bioactive compounds, drugs, antibiotics, other antimicrobial agents, extracts, essential oils into protein- polysaccharide-based nanocarriers. These show benefits human health, especially successful anticancer activity. The article, divided protein-based further according biopolymer, enables reader select appropriate more easily incorporation desired component. latest research results from last five years in field production loaded with healthcare applications included review.

Language: Английский

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Metal‐Phenolic Network with Pd Nanoparticle Nodes Synergizes Oxidase‐Like and Photothermal Properties to Eradicate Oral Polymicrobial Biofilm‐Associated Infections

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(7)

Published: Nov. 10, 2023

Designing an effective treatment strategy to combat oral diseases caused by complex polymicrobial biofilms remains a great challenge. Herein, series of metal-phenolic network with Pd nanoparticle nodes using polyphenols as stabilizers and reducing agents is constructed. Among them, sulfonated lignin-Pd (SLS-Pd) ultrafine size palladium nanoparticles broadband near infrared absorption exhibit excellent oxidase-like activity stable photothermal effect. In vitro experiments demonstrate that the superoxide radical generated SLS-Pd exhibits selective antibacterial effects, while its effect induced hyperthermia potent antifungal properties. This difference further elucidated RNA-sequencing analysis all-atom simulation. Moreover, SLS-Pd-mediated synergistic antimicrobial system remarkable efficacy in combating various biofilms. By establishing root canal model oropharyngeal candidiasis model, feasibility treating biofilm-related infections validated. provides promising therapeutic approach for biofilm-associated cavity.

Language: Английский

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Antimicrobial Hybrid Amphiphile via Dynamic Covalent Bonds Enables Bacterial Biofilm Dispersal and Bacteria Eradication

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(23)

Published: Feb. 28, 2023

Abstract To tackle the problems caused by bacterial biofilms, herein, this study reports an antimicrobial hybrid amphiphile (aHA) via dynamic covalent bonds for eradicating staphylococcal biofilms. aHA is synthesized iminoboronate ester formation between DETA NONOate (nitric oxide donor), 3 4‐dihydroxybenaldehyde, and phenylboronic acid‐modified ciprofloxacin (Cip). can self‐assemble in aqueous solution with ultra‐small critical aggregation concentration of 3.80 × 10 –5 m high drug loading content 73.8%. The sensitive to acidic oxidative biofilm microenvironment, liberating nitric Cip that synergistically eradicate end, assemblies efficiently infections ameliorate inflammation murine peritoneal subcutaneous infection models without any notable side effects on normal tissues. Collectively, may provide a facile efficient alternative current development anti‐biofilm therapies.

Language: Английский

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