Nanoenzyme-Reinforced Injectable Hydrogel for Healing Diabetic Wounds Infected with Multidrug Resistant Bacteria

Nano Letters, Journal Year: 2020, Volume and Issue: 20(7), P. 5149 - 5158

Published: June 23, 2020

Diabetic wound healing remains a critical challenge due to its vulnerability multidrug-resistant (MDR) bacterial infection, as well the hyperglycemic and oxidative microenvironment. Herein, an injectable multifunctional hydrogel (FEMI) was developed simultaneously overcome these hurdles. The FEMI fabricated through Schiff-based reaction between ε-polylysine (EPL)-coated MnO2 nanosheets (EM) insulin-loaded self-assembled aldehyde Pluronic F127 (FCHO) micelles. Through synergistic combination of EPL "nanoknife-like" nanosheets, exhibited extraordinary antimicrobial capacities against MDR bacteria. nanoenzyme reshaped hostile microenvironment by decomposing endogenous H2O2 into O2. Meanwhile, pH/redox dual-responsive achieved sustained spatiotemporal controlled release insulin regulate blood glucose. Our demonstrated accelerated bacteria-infected diabetic in vivo represents versatile strategy for broad range tissue damages caused diabetes.

Language: Английский

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Supramolecular Adhesive Hydrogels for Tissue Engineering Applications

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(6), P. 5604 - 5640

Published: Jan. 13, 2022

Tissue engineering is a promising and revolutionary strategy to treat patients who suffer the loss or failure of an organ tissue, with aim restore dysfunctional tissues enhance life expectancy. Supramolecular adhesive hydrogels are emerging as appealing materials for tissue applications owing their favorable attributes such tailorable structure, inherent flexibility, excellent biocompatibility, near-physiological environment, dynamic mechanical strength, particularly attractive self-adhesiveness. In this review, key design principles various supramolecular strategies construct comprehensively summarized. Thereafter, recent research progress regarding applications, including primarily dermal repair, muscle bone neural vascular oral corneal cardiac fetal membrane hepatic gastric systematically highlighted. Finally, scientific challenges remaining opportunities underlined show full picture hydrogels. This review expected offer comparative views critical insights inspire more advanced studies on pave way different fields even beyond applications.

Language: Английский

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Artificial Nonenzymatic Antioxidant MXene Nanosheet-Anchored Injectable Hydrogel as a Mild Photothermal-Controlled Oxygen Release Platform for Diabetic Wound Healing

ACS Nano, Journal Year: 2022, Volume and Issue: 16(5), P. 7486 - 7502

Published: May 9, 2022

Hypoxia, excessive reactive oxygen species (ROS), impaired angiogenesis, lasting inflammation, and bacterial infection, are key problems impeding diabetic wound healing. Particularly, controllable release ROS scavenging capacities critical during the healing process. Here, an injectable hydrogel based on hyaluronic acid-graft-dopamine (HA-DA) polydopamine (PDA) coated Ti3C2 MXene nanosheets is developed catalytically cross-linked by oxyhemoglobin/hydrogen (HbO2/H2O2) system combined with mild photothermal stimulation for HbO2 not only acts as a horseradish peroxidase-like to catalyze formation but also carrier controllably when activated heat produced from near-infrared (NIR) irradiation. Specifically, can provide repeatedly binding in air NIR off. The stable photoresponsive heating behavior of ensures repeatable release. Additionally, artificial nonenzymatic antioxidant proposed scavenge nitrogen including H2O2, O2•-, •OH, keeping intracellular redox homeostasis alleviating oxidative stress, eradicate bacteria avoid infection. antibacterial abilities further improved PDA coating, which promotes cross-linking into network hydrogel. HA-DA molecules endow capacity regulate macrophage polarization M1 M2 achieve anti-inflammation. More importantly, MXene-anchored multifunctions tissue adhesion, self-healing, injectability, hemostasis, stimulation, greatly human umbilical vein endothelial cell proliferation migration notably facilitates infected

Language: Английский

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Selection of Appropriate Wound Dressing for Various Wounds

Frontiers in Bioengineering and Biotechnology, Journal Year: 2020, Volume and Issue: 8

Published: March 19, 2020

There are many factors involved in wound healing, and the healing process is not static. The therapeutic effect of modern dressings clinical management wounds documented. However, there few reports regarding reasonable selection for certain types clinic. In this article, we retrospect history dressing development classification dressings. addition, pros cons mainstream different wounds, such as diabetic foot ulcers, pressure burns scalds, chronic leg well physiological mechanisms summarized. This article provides a guideline selecting suitable according to wounds.

Language: Английский

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<p>Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomes Combined Pluronic F127 Hydrogel Promote Chronic Diabetic Wound Healing and Complete Skin Regeneration</p>

International Journal of Nanomedicine, Journal Year: 2020, Volume and Issue: Volume 15, P. 5911 - 5926

Published: Aug. 1, 2020

Chronic refractory wounds are a multifactorial comorbidity of diabetes mellitus with the characteristic impaired vascular networks. Currently, there is lack effective treatments for such wounds. Various types mesenchymal stem cell-derived exosomes (MSC-exos) have been shown to exert multiple therapeutic effects on skin regeneration. We aimed determine whether constructed combination human umbilical cord MSC (hUCMSC)-derived (hUCMSC-exos) and Pluronic F-127 (PF-127) hydrogel could improve wound healing.We topically applied cord-derived encapsulated in thermosensitive PF-127 full-thickness cutaneous streptozotocin-induced diabetic rat model. The material properties healing ability cellular responses were analyzed.Compared hUCMSC-exos, PF-127-only or control treatment, hUCMSC-exos resulted significantly accelerated closure rate, increased expression CD31 Ki67, enhanced regeneration granulation tissue upregulated endothelial growth factor (VEGF) transforming beta-1 (TGFβ-1).The efficient delivery gel improved exosome promote healing. Thus, this biomaterial-based therapy may represent new approach chronic

Language: Английский

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Wound Dressing: From Nanomaterials to Diagnostic Dressings and Healing Evaluations

ACS Nano, Journal Year: 2022, Volume and Issue: 16(2), P. 1708 - 1733

Published: Jan. 20, 2022

Wound dressings based on nanomaterials play a crucial role in wound treatment and are widely used whole range of medical settings, from minor to life-threatening tissue injuries. This article presents an educational review the accumulating knowledge this multidisciplinary area lay out challenges opportunities that lie ahead ignite further faster development clinically valuable technologies. The analyzes functional advantages nanomaterial-based gauzes hydrogels as well hybrid structures thereof. On basis, state-of-the-art advances transfer (semi)blind approaches evaluation state smart enable real-time monitoring diagnostic functions could help during healing. explores translation related aspects into real-world use. ongoing future associated with clinical decisions presented reviewed.

Language: Английский

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Engineering Bioactive M2 Macrophage‐Polarized Anti‐Inflammatory, Antioxidant, and Antibacterial Scaffolds for Rapid Angiogenesis and Diabetic Wound Repair

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 31(30)

Published: May 26, 2021

Abstract Diabetic wound healing still faces great challenges due to the excessive inflammation, easy infection, and impaired angiogenesis in beds. The immunoregulation of macrophages polarization toward M2 phenotype that facilitates transition from inflammation proliferation phase has been proved be an effective way improve diabetic healing. Herein, phenotype‐enabled anti‐inflammatory, antioxidant, antibacterial conductive hydrogel scaffolds (GDFE) for producing rapid repair are reported. GDFE fabricated facilely through dynamic crosslinking between polypeptide polydopamine graphene oxide. possess thermosensitivity, self‐healing behavior, injectability, broad‐spectrum activity, antioxidant anti‐inflammatory ability, electronic conductivity. effectively activates significantly promotes dermal fibroblasts, migration, vitro endothelial cells paracrine mechanisms. vivo results a full‐thickness model demonstrate can rapidly promote skin regeneration, fast anti‐inflammation macrophage polarization. This study provides highly efficient strategy treating designing polarization‐enabled bioactive materials.

Language: Английский

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Wound microenvironment self-adaptive hydrogel with efficient angiogenesis for promoting diabetic wound healing

Bioactive Materials, Journal Year: 2022, Volume and Issue: 20, P. 561 - 573

Published: July 1, 2022

Neovascularization is critical to improve the diabetic microenvironment, deliver abundant nutrients wound and promote closure. However, excess of oxidative stress impedes healing process. Herein, a self-adaptive multifunctional hydrogel with self-healing property injectability fabricated through boronic ester-based reaction between phenylboronic acid groups 3-carboxyl-4-fluorophenylboronic -grafted quaternized chitosan hydroxyl polyvinyl alcohol, in which pro-angiogenic drug desferrioxamine (DFO) loaded form gelatin microspheres (DFO@G). The ester bonds can self-adaptively react hyperglycemic hydrogen peroxide alleviate release DFO@G early phase healing. A sustained DFO then realized by responding overexpressed matrix metalloproteinases. In full-thickness model, accelerates angiogenesis upregulating expression hypoxia-inducible factor-1 angiogenic growth factors, resulting collagen deposition rapid This not only change microenvironment pro-healing state decreasing stress, but also respond metalloproteinases DFO. has potential for treating wounds.

Language: Английский

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Bioactive Anti-inflammatory, Antibacterial, Antioxidative Silicon-Based Nanofibrous Dressing Enables Cutaneous Tumor Photothermo-Chemo Therapy and Infection-Induced Wound Healing

ACS Nano, Journal Year: 2020, Volume and Issue: 14(3), P. 2904 - 2916

Published: Feb. 7, 2020

Traditional skin tumor surgery and chronic bacterial-infection-induced wound healing/skin regeneration is still a challenge. The ideal strategy should eliminate the tumor, enhance formation, be anti-infection. Herein, we designed multifunctional elastomeric poly(l-lactic acid)–poly(citrate siloxane)–curcumin@polydopamine hybrid nanofibrous scaffold (denoted as PPCP matrix) for tumor-infection therapy infection-induced healing. matrix showed intrinsically properties including antioxidative, anti-inflammatory, photothermal, antibacterial, anticancer, angiogenesis bioactivities. polydopamine/curcumin presented an excellent near-infrared photothermal/cancer cell toxicity capacity, respectively, which supported synergetic antibacterial in vitro/in vivo. Additionally, significantly promotes adhesion proliferation of normal cells accelerates cutaneous healing mice bacterial-infected by enhancing early angiogenesis. with bioactivities provides competitive

Language: Английский

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Natural Polymer-Based Antimicrobial Hydrogels without Synthetic Antibiotics as Wound Dressings

Biomacromolecules, Journal Year: 2020, Volume and Issue: 21(8), P. 2983 - 3006

Published: July 16, 2020

Wound healing is usually accompanied by bacterial infection. The excessive use of synthetic antibiotics leads to drug resistance, posing a significant threat human health. Hydrogel-based wound dressings aimed at mitigating infections have emerged as an effective treatment. review presented herein particularly focuses on the hydrogels originating from natural polymers. To further enhance performance dressings, various strategies and approaches been developed endow with excellent broad-spectrum antibacterial activity. Those that are summarized in current intrinsic or stimuli-triggered bactericidal properties others serve vehicles for loading agents without antibiotics. Specific attention paid antimicrobial mechanisms hydrogels. Practical applications accelerate employing these antibiotic-free also introduced along discussion challenges perspectives leading new technologies.

Language: Английский

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