Accessing Different Protein Conformer Ensembles with Tunable Capillary Vibrating Sharp-Edge Spray Ionization DOI Creative Commons
Daud Sharif, Vikum K. Dewasurendra,

Mst Nigar Sultana

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Capillary vibrating sharp-edge spray ionization (cVSSI) has been used to control the droplet charging of nebulized microdroplets and monitor effects on protein ion conformation makeup as determined by mass spectrometry (MS). Here it is observed that application voltage results in noticeable differences charge state distributions (CSDs) ubiquitin ions. The data can be described most generally three distinct regions: Under low-voltage conditions (<+200 V, LV regime), low states (2+ 4+ ions) dominate spectra. For midvoltage (+200 +600 MV higher (7+ 12+ are observed. high-voltage (>+600 HV "nano-electrospray (nESI)-type distribution" achieved which 6+ 5+ species dominant Analysis these suggests different pathways progeny nanodroplet production result regime, aerodynamic breakup leads droplets selective for native solution ensemble (minus multimeric species). In large persist longer periods time, leading heating a shift partially unfolded species. access nanodroplets faster, sampling indicated nESI-type CSD. notable observation limited multimer formation adduct regime hypothesized from aero resulting carrier partitioning sampled droplets. tunable afforded cVSSI presents opportunities study charge, size, spectrometer inlet temperature conformer spectrometer. Additionally, approach may provide tool rapid comparison stabilities.

Language: Английский

Accessing Different Protein Conformer Ensembles with Tunable Capillary Vibrating Sharp-Edge Spray Ionization DOI Creative Commons
Daud Sharif, Vikum K. Dewasurendra,

Mst Nigar Sultana

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Capillary vibrating sharp-edge spray ionization (cVSSI) has been used to control the droplet charging of nebulized microdroplets and monitor effects on protein ion conformation makeup as determined by mass spectrometry (MS). Here it is observed that application voltage results in noticeable differences charge state distributions (CSDs) ubiquitin ions. The data can be described most generally three distinct regions: Under low-voltage conditions (<+200 V, LV regime), low states (2+ 4+ ions) dominate spectra. For midvoltage (+200 +600 MV higher (7+ 12+ are observed. high-voltage (>+600 HV "nano-electrospray (nESI)-type distribution" achieved which 6+ 5+ species dominant Analysis these suggests different pathways progeny nanodroplet production result regime, aerodynamic breakup leads droplets selective for native solution ensemble (minus multimeric species). In large persist longer periods time, leading heating a shift partially unfolded species. access nanodroplets faster, sampling indicated nESI-type CSD. notable observation limited multimer formation adduct regime hypothesized from aero resulting carrier partitioning sampled droplets. tunable afforded cVSSI presents opportunities study charge, size, spectrometer inlet temperature conformer spectrometer. Additionally, approach may provide tool rapid comparison stabilities.

Language: Английский

Citations

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