Novel Label-Free Nanocrystalline Gold Interdigitated Microelectrode Immunosensor for the Rapid and Ultrasensitive Detection of SARS-CoV-2 DOI
Sihan Wang,

Yougang Zhong,

Zhen Gong

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(8), P. 2933 - 2944

Published: July 5, 2023

Waves of COVID-19 outbreaks have dragged down the global economy and endangered human life. There is an urgent need for timeliness sensitive SARS-CoV-2 detection techniques to complement existing PCR assay. Herein, controllable growth gold crystalline grains was achieved by applying reverse current during pulse electrochemical deposition (PED) interval. The proposed method validates effects (PRC) on atomic arrangement, crystal structures, orientations, film characteristics in Au PED. gap between surface nanocrystalline interdigitated microelectrodes (NG-IDME) fabricated PED+PRC process matches size antiviral antibody. Immunosensors are prepared binding a large number antibodies NG-IDME. NG-IDME immunosensor has high specific capture ability nucleocapsid protein (SARS-CoV-2/N-Pro) completes ultrasensitive quantification SARS-CoV-2/N-Pro humans pets within 5 min (the LOQ as low 75 fg/mL). specificity, accuracy, stability, actual blind sample tests show that suitable animals. This approach assists monitoring transmission SARS-CoV-2-infected animals humans.

Language: Английский

Editors’ Choice—Challenges and Opportunities for Developing Electrochemical Biosensors with Commercialization Potential in the Point-of-Care Diagnostics Market DOI Creative Commons
A. Akhlaghi, Harmanjit Kaur, Bal Ram Adhikari

et al.

ECS Sensors Plus, Journal Year: 2024, Volume and Issue: 3(1), P. 011601 - 011601

Published: March 1, 2024

There is a plethora of electrochemical biosensors developed for ultrasensitive detection clinically relevant biomarkers. However, many these systems lose their performance in heterogeneous clinical samples and are too complex to be operated by end users at the point-of-care (POC), prohibiting commercial success. Integration with sample processing technology addresses both challenges; however, it adds manufacturing complexity overall cost systems. Herein, we review different components biosensor avenues creating fully integrated In context integration, focus on discussing trade-offs between sensing performance, cost, scalable guide readers toward designing new commercialization potential.

Language: Английский

Citations

38
Aptamer-based assembly systems for SARS-CoV-2 detection and therapeutics DOI

Yuhang Dong,

Jingping Wang,

Ling Chen

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(13), P. 6830 - 6859

Published: Jan. 1, 2024

This review presents the recent progress on aptamers that have been explored for SARS-CoV-2 detection and therapeutics, wherein construction principles characteristics of aptamer-based assembly systems are systematically summarized.

Language: Английский

Citations

15
High‐Precision Viral Detection Using Electrochemical Kinetic Profiling of Aptamer‐Antigen Recognition in Clinical Samples and Machine Learning DOI Creative Commons
Payel Sen, Zijie Zhang, Sadman Sakib

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(20)

Published: March 9, 2024

Abstract High‐precision viral detection at point of need with clinical samples plays a pivotal role in the diagnosis infectious diseases and control global pandemic. However, complexity that often contain very low concentrations makes it huge challenge to develop simple diagnostic devices do not require any sample processing yet are capable meeting performance metrics such as high sensitivity specificity. Herein we describe new single‐pot single‐step electrochemical method uses real‐time kinetic profiling interaction between high‐affinity aptamer an antigen on surface. This generates many data points per sample, which when combined machine learning, can deliver highly accurate test results short testing time. We demonstrate this concept using both SARS‐CoV‐2 Influenza A viruses model specifically engineered aptamers. Utilizing technique diagnose COVID‐19 37 real human saliva specificity 100 % (27 true negatives 10 positives, 0 false negative positive), showcases superb precision method.

Language: Английский

Citations

13
Electrochemical Sensor for the Detection and Accurate Early Diagnosis of Ovarian Cancer DOI

Feng Ge,

Ding Wei, Cong Han

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(6), P. 2897 - 2906

Published: May 22, 2024

Ovarian cancer (OC) has the highest mortality rate among malignant tumors, primarily because it is difficult to diagnose early. Exosomes, a type of extracellular vesicle rich in parental information, have garnered significant attention field diagnosis and treatment. They play an important regulatory role occurrence, development, metastasis OC. Consequently, exosomes emerged as noninvasive biomarkers for early detection. Therefore, identifying cancer-derived may offer novel biomarker detection In this study, we developed metal–organic frameworks assembled "double hook"-type aptamer electrochemical sensor, which enables accurate Under optimal experimental conditions, impedance spectroscopy technology demonstrated good linear relationship within concentration range 31–3.1 × 106 particles per microliter, with limit low 12 microliter. The universal exosome platform constructed, can not only differentiate between high-grade serous ovarian (HGSOC) patients healthy individuals but also distinguish HGSOC nonhigh-grade OC (non-HGSOC). provides strategy holds great significance clinical differential diagnosis.

Language: Английский

Citations

11
SERS/EC Dual-Mode Sensing System for the Assay of SARS-CoV-2 RNA by Using Single Gold Nanowires as a Platform DOI

Qingshan Dai,

Jiarui Huang, Xia Qiu

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(6), P. 6369 - 6379

Published: March 12, 2024

The continuous spread and infection of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) worldwide has brought a great impact on people's lives health become serious public problem. In this contribution, surface-enhanced Raman scattering/electrochemical (SERS/EC) dual-mode sensing system using single Au nanowires (Au NWs) as platform is proposed for detecting SARS-CoV-2 RNA with excellent sensitivity selectivity. NWs modified bimetallic NPs can significantly increase SERS/EC signals, presence produce attenuated signals by shearing off aptamer containing signal molecules. addition to its specific recognition high (detection limits 1.02 pM SERS method 0.21 EC method), nanosensor been successfully applied samples complex matrices expected serve potential, sensitive, reliable tool virus screening protein detection, especially in vivo detection due small overall dimension NWs.

Language: Английский

Citations

5
Functional nucleic acid-based biosensors for virus detection DOI Creative Commons
Zijie Zhang, Bal Ram Adhikari, Payel Sen

et al.

Advanced Agrochem, Journal Year: 2023, Volume and Issue: 2(3), P. 246 - 257

Published: July 16, 2023

A growing number of viruses pose significant threats to human health, impact agricultural production and compromise food safety. Minimizing the viral infections demands effective technologies that can deliver sensitive detection under various settings. Functional nucleic acids (FNAs) – natural or synthetic acid molecules with catalytic and/or binding properties have been increasingly explored as key components for setting up diagnostic tests detection. FNA-based biosensors offer distinct advantages, such ability achieve in complex biological samples, ease use, scalability, cost-effectiveness. This mini-review will be dedicated appraising progress made recent years diagnosing diverse animal viruses. We first describe virus structures potential biomarkers utilized targets FNA probe engineering. is followed by reviewing latest advances two classes FNAs - DNAzyme DNA aptamers, focuses on DNAzyme- aptamer-based employ signal transduction mechanisms detections. Finally, we discuss current challenges field suggest future research directions address these challenges.

Language: Английский

Citations

12
Preconcentration and detection of SARS-CoV-2 in wastewater: A comprehensive review DOI
Yihan Yang, Jisui Tan, F. Wang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116617 - 116617

Published: July 30, 2024

Language: Английский

Citations

4
Recent Technologies for the Determination of SARS‐CoV‐2 in Wastewater DOI Creative Commons

Nolwazi T. Gazu,

Aoife Morrin, Xolile Fuku

et al.

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(13)

Published: April 1, 2025

Abstract The COVID‐19 pandemic outbreak raised major concerns in public health globally, raising the crucial need for development of methods to monitor spread communities worldwide. Wastewater‐based epidemiology (WBE) surveillance has been used as a novel tool outbreaks because its affordability and efficiency tracking infectious contaminants. Unlike other means tracking, wastewater is independent individuals having accessibility healthcare, doctor visits, or infection testing availability. Consequently, considerable awareness complete infections, including at community level. In WBE studies, polymerase chain reaction‐based (PCR) techniques are referred “gold‐standard” method detecting SARS‐CoV‐2 many countries. Nevertheless, despite extensive sensitive selective PCR‐based methods, these have shown some limitations that hinder their application, such requirement repeated heating cooling cycles analysis time 3–4 h. alternative do not rely on same consumables conventionally employed electrochemical biosensing, environmental water samples offers favorable advantages improved turnaround times portability. However, currently highly focused clinical applications than wastewater. This review focuses disadvantages associated with conventional alternative: electrochemical‐bioreceptor‐based technique SARS‐CoV‐2. addition, highlights broad use WBE, binding affinity various bioreceptors toward viral proteins, enhancing analytical properties biosensors integration techniques. integrated systems, especially, electrochemical‐CRISPR based, high sensitivities (down concentrations atto‐molar), potential application low‐resource areas.

Language: Английский

Citations

0
Point-of-care Diagnostics for SARS-CoV-2 Wastewater-based Epidemiology: A big Leap toward Miniaturization DOI
Ahmed Donia, Nonsikelelo Precios Mthethwa, Isaac Amoah

et al.

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Integrating point-of-care diagnostics into SARS-CoV-2 wastewater surveillance enables rapid detection and response. This innovation enhances pandemic management has potential for broader public health applications beyond COVID-19.

Language: Английский

Citations

1
Electrochemical biosensors: rapid detection methods in wastewater-based epidemiology research DOI

Chunying Yuan,

Mushui Shu, Zhaohui Fu

et al.

Environmental Science Water Research & Technology, Journal Year: 2023, Volume and Issue: 10(2), P. 316 - 338

Published: Dec. 8, 2023

The fundamentals, sensing mechanisms, strategies for improving the performance and representative discoveries of electrochemical biosensors monitoring various targets are summarized.

Language: Английский

Citations

2