Published: Jan. 1, 2024
Language: Английский
Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
This review explores how AI addresses challenges in biosynthetic pathway research, accelerating the development of bioactive natural products for pharmacology, agriculture, and biotechnology.
Language: Английский
Citations
0
World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)
Published: March 28, 2025
Language: Английский
Citations
0
Biomedical engineering, Journal Year: 2024, Volume and Issue: unknown
Published: June 28, 2024
The impact of computer-aided drug designing in the field medicinal chemistry has created a boon discovery process. Molecular docking is an integral part bioinformatics that deals with protein-ligand interactions, binding conformations, and affinity predictions. It shown to be rapid, easy, affordable method business research settings alike. advancement hardware software led enhanced molecular dynamic simulations thus authenticate computational results. This great minimizing cost time involved also helped identifying rationale for repurposing approaches. chapter will give in-depth knowledge importance discovery, their on repurposing, success stories silico approach repurposing.
Language: Английский
Citations
3
Bioresource Technology, Journal Year: 2024, Volume and Issue: unknown, P. 131703 - 131703
Published: Oct. 1, 2024
Language: Английский
Citations
3
Synthetic and Systems Biotechnology, Journal Year: 2025, Volume and Issue: 10(2), P. 421 - 432
Published: Jan. 18, 2025
Streptomyces sp. MJM3502 is a promising producer of rufomycins, which are class potent anti-tuberculosis lead compounds. Although the structure, activity, and mechanism main rufomycin 4/6 its analogs have been extensively studied, significant gap remains in our understanding genome sequence biosynthetic pathway MJM3502, metabolic engineering has not yet reported. This study established genetic manipulation platform for strain. Using CRISPR/Cas9-based technology to in-frame insert strong kasO∗p promoter upstream rufB rufS genes BGC, we increased production by 4.1-fold 2.8-fold, respectively. Furthermore, designing recombinant strains inserting encoding cytochrome P450 enzymes led new derivatives. These findings provide basis enhancing valuable natural compounds offer insights into generation novel active products via synthetic biology engineering.
Language: Английский
Citations
0
Sustainable Food Technology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
AI significantly transforms the food business by optimizing production processes of therapeutic Ganoderma spp. and improving quality safety control based functional food.
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 17, 2025
The genus Epimedium includes popular Chinese medicinal plants, and icariin its precursor icaritin are the key bioactive components of Epimedium. Here, we identified flavonoid 8-dimethylallyltransferase (F8DT) in authentic material koreanum, which is a gene biosynthesis pathway. This enzyme can catalyze synthesis 8-prenylkaempferol (8PK) from kaempferol. catalytic ability rate-limiting EkF8DT was significantly improved by truncating N-terminal intrinsically disordered regions (IDRs) enhancing flux mevalonate Icaritin also successfully synthesized introducing 4'-O-methyltransferase into Saccharomyces cerevisiae strain. Finally, highest production 8PK (3.6 g/L 172.0 mg/L, respectively) obtained S.
Language: Английский
Citations
0
Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103137 - 103137
Published: April 26, 2024
Language: Английский
Citations
3
Frontiers in Hematology, Journal Year: 2024, Volume and Issue: 3
Published: April 4, 2024
Thalassemia is one of the inherited hemoglobin disorders worldwide, resulting in ineffective erythropoiesis, chronic hemolytic anemia, compensatory hemopoietic expansion, hypercoagulability, etc., and when a mother carries thalassemia gene, child more likely to have severe thalassemia. Furthermore, economic time costs genetic testing for prevent many patients from being diagnosed time. To solve this problem, we performed least absolute shrinkage selection operator (LASSO) regression analyze correlation between blood routine indicators containing mean corpuscular volume (MCV), (MCH), concentration (MCHC), red cell (RBC). We then built nomogram predict occurrence thalassemia, receiver operating characteristic (ROC) curve was used verify prediction efficiency model. In total, obtained 7,621 cases, including 847 6,774 non-thalassemia. Among patients, with positivity rate 67.2%, 569 cases were positive α-thalassemia, 31.5%, 267 β-thalassemia. The remaining 11 both α- Based on machine learning algorithm, screened four optimal indicators, namely, MCV, MCH, RBC, MCHC. AUC value MCHC 0.907, 0.906, 0.796, 0.795, respectively. Moreover, model 0.911. summary, novel effective which functioned accurately, may provide new insights early screening future.
Language: Английский
Citations
2
Pharmaceutical Science Advances, Journal Year: 2024, Volume and Issue: 2, P. 100050 - 100050
Published: Aug. 24, 2024
Language: Английский
Citations
2