Fluorogenic Tetrazine Bioorthogonal Probes for Advanced Application in Bioimaging and Biomedicine

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

A variety of bioorthogonal chemical tools have been developed and widely used in the study biological phenomena situ. Tetrazine chemistry exhibits ultrafast reaction kinetics, excellent biocompatibility, precise optical regulatory capabilities. Fluorogenic tetrazine probes achieved particularly diverse applications bioimaging disease diagnosis treatment. This Viewpoint briefly introduces characteristics advantages chemistry, some design strategies fluorogenic probes, status these to vivo imaging, as well Finally, we discuss challenges propose future trends field probes. offers insights into development new for biology research drugs.

Language: Английский

---

Biocatalytic Generation of Trifluoromethyl Radicals by Nonheme Iron Enzymes for Enantioselective Alkene Difunctionalization

Published: Aug. 16, 2024

The trifluoromethyl (–CF3) group represents a highly prevalent functionality in pharmaceuticals. Over the past few decades, significant advances have been made development of synthetic methods for trifluoromethylation. In contrast, there are currently no metal-loenzymes known to catalyze formation C(sp3)‒CF3 bonds. this work, we demonstrate that nonheme iron enzyme, hydroxy-mandelate synthase from Amycolatopsis orientalis (AoHMS), is capable generating CF3 radicals hypervalent iodine(III) reagents and directing them enantioselective alkene azidation. A high-throughput screening (HTS) platform based on Staudinger ligation was established, enabling rapid evaluation AoHMS variants abiological transformation. final opti-mized variant accepts range substrates, producing azidation products up 73% yield 96:4 enanti-omeric ratio (e.r.). biocatalytic can be further extended pentafluoroethyl diazidation by altering reagent. addition, anion competition experiments provide insights into radical rebound process This study not only expands catalytic repertoire metalloenzymes transformations but also creates new enzymatic space organofluorine synthesis.

Language: Английский

---

Fluorogenic Coumarins Activated via Bioorthogonal Reaction

Published: Aug. 19, 2024

Fluorogenic bioorthogonal reagents enable facile detection in complex environments. While useful for real-time imaging, few such probes are available. Existing tools also exploit similar mechanisms signal turn-on, precluding multiplexed applications. To address these gaps, we developed a palette of cyclopropenone (CpO) scaffolds that activated by phosphines to provide coumarins. The top CpO-phosphine pairs show strong fluorescent enhancements (up 760-fold) and high quantum yields, stable aqueous CpO fluorogens exhibit unique reaction profiles, setting the stage multi-component labeling studies.

Language: Английский

---

Formaldehyde quantification using gas chromatography–mass spectrometry reveals high background environmental formaldehyde levels

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 4, 2024

Language: Английский

---

Multiplexed No-Wash Cellular Imaging using BenzoTag, an Evolved Self-Labeling Protein

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

BenzoTag, a self-labeling protein developed using molecular evolution, enables wash-free labeling in live cells seconds low nanomolar dye concentrations.

Language: Английский

---