Smartphone-Based Electrochemical Immunoassay for Point-of-Care Detection of SARS-CoV-2 Nucleocapsid Protein

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(43), P. 15155 - 15161

Published: Oct. 17, 2022

Large-scale, rapid, and inexpensive serological diagnoses of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) are great interest in reducing virus transmission at the population level; however, their development is greatly plagued by lack available point-of-care methods, leading to low detection efficiency. Herein, an ultrasensitive smartphone-based electrochemical immunoassay reported for rapid (less than 5 min), low-cost, easy-to-implement SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 N protein). Specifically, was fabricated on a screen-printed carbon electrode coated with electrodeposited gold nanoparticles, followed incubation anti-N antibody (Ab) bovine serum albumin as working electrode. Accompanied antigen-antibody reaction between Ab, electron transfer electroactive species [Fe(CN)6]3-/4- surface disturbed, resulting reduced square-wave voltammetry currents 0.075 V versus Ag/AgCl reference The proposed provided good linear range concentrations within scope 0.01-1000 ng/mL (R2 = 0.9992) limit down 2.6 pg/mL. Moreover, data wirelessly transmitted interface smartphone, corresponding concentration value calculated displayed. Therefore, portable mode offers potential self-differential diagnosis residents, which will facilitate effective control large-scale screening resource-limited areas.

Language: Английский

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Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(11), P. 10319 - 10392

Published: April 12, 2022

The evolutionary success in information technology has been sustained by the rapid growth of sensor technology. Recently, advances have promoted ambitious requirement to build intelligent systems that can be controlled external stimuli along with independent operation, adaptivity, and low energy expenditure. Among various sensing techniques, field-effect transistors (FETs) channels made two-dimensional (2D) materials attract increasing attention for advantages such as label-free detection, fast response, easy capability integration. With atomic thickness, 2D restrict carrier flow within material surface expose it directly environment, leading efficient signal acquisition conversion. This review summarizes latest 2D-materials-based FET (2D FET) sensors a comprehensive manner contains material, operating principles, fabrication technologies, proof-of-concept applications, prototypes. First, brief description background fundamentals is provided. subsequent contents summarize physical, chemical, biological their applications. Then, we highlight challenges commercialization discuss corresponding solution techniques. following section presents systematic survey recent progress developing commercial Lastly, long-standing efforts prospective future development FET-based toward commercialization.

Language: Английский

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Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(12), P. 7953 - 8039

Published: June 1, 2023

Whole blood, as one of the most significant biological fluids, provides critical information for health management and disease monitoring. Over past 10 years, advances in nanotechnology, microfluidics, biomarker research have spurred development powerful miniaturized diagnostic systems whole blood testing toward goal monitoring treatment. Among techniques employed whole-blood diagnostics, electrochemical biosensors, known to be rapid, sensitive, capable miniaturization, reagentless washing free, become a class emerging technology achieve target detection specifically directly complex media, e.g., or even living body. Here we are aiming provide comprehensive review summarize over decade sensors analysis. Further, address remaining challenges opportunities integrate sensing platforms.

Language: Английский

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Integrated Urinalysis Devices Based on Interface‐Engineered Field‐Effect Transistor Biosensors Incorporated With Electronic Circuits

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(36)

Published: July 19, 2022

Urinalysis is attractive in non-invasive early diagnosis of bladder cancer compared with clinical gold standard cystoscopy. However, the trace tumor biomarkers urine and particularly complex environment pose significant challenges for urinalysis. Here, a clinically adoptable urinalysis device that integrates molecular-specificity indium gallium zinc oxide field-effect transistor (IGZO FET) biosensor arrays, control panel, an internet terminal directly analyzing five bladder-tumor-associated proteins samples, reported classification. The IGZO FET biosensors engineered sensing interfaces provide high sensitivity selectivity identification environment. Integrating machine-learning algorithm, this can identify accuracy 95.0% cohort 197 patients 75 non-bladder individuals, distinguishing stages overall 90.0% assessing recurrence after surgical treatment. defines robust technology remote healthcare personalized medicine.

Language: Английский

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Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs

Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(10)

Published: May 1, 2024

Abstract Early lung cancer screening by computed tomography is hampered pulmonary nodules caused massive COVID‐19 infections, necessitating an ultrasensitive approach for the early diagnosis of cancers at single‐base level from circular tumor DNAs (ctDNAs). This study introduces that merges DNA origami and scissors technologies in a framework surface plasmon resonance (SPR) biosensors. By combining precision probes with inherent resolution scissors, this method systematically addresses limitations conventional SPR techniques, resulting enhanced detection accuracy. The synergistic interplay between enables biosensors to achieve unprecedented levels sensitivity, precision, practical utility. efficacy allows precise identification mutations, demonstrated here T790M mutation EGFR gene G12C KRAS non‐small cell patients. With technique, as well near zeptomolar‐level sensitivity achieved. As result, discovery holds significant potential advance field diagnostics.

Language: Английский

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Functional probes for the diagnosis and treatment of infectious diseases

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Abstract Infectious diseases present significant challenges to global health, thereby extensively affecting both human society and the economy. In recent years, functional probes have demonstrated remarkable potential as crucial biomedical media for research treatment of infectious diseases. Their applications in realm include pathogen detection, exploration biological mechanisms, development anti‐infective drugs. This review provides a concise introduction severity, classification, pathogenesis Subsequently, we examined distinctiveness design strategies diagnosing treating diseases, shedding light on their rationale using typical examples. We discuss current status associated with clinical implementation probes. Furthermore, explored prospects these diagnosis aims offer novel insights into diagnostic broaden disease treatment.

Language: Английский

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Recent Advances in Graphene Field‐Effect Transistor Toward Biological Detection

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(44)

Published: May 22, 2024

Abstract Recently, field‐effect transistors (FETs) have emerged as a novel type of multiparameter, high‐performance, highly integrated platform for biochemical detection, leveraging their classical three‐terminal structure, working principles, and fabrication methods. Notably, graphene materials, known exceptional electrical optical properties well biocompatibility, serve fundamental component these devices, further enhancing advantages in biological detection. This review places special emphasis on recent advancements transistor (GFET)‐based biosensors focuses four main areas: i) the basic concepts FETs specific GFETs; ii) various state‐of‐the‐art approaches to enhance performance GFET‐based terms operating principles “3S”—stability, sensitivity, specificity; iii) multiplexed detection strategies biosensors; iv) current challenges future perspectives field biosensors. It is hoped that this article can profoundly elucidate development GFET inspire broader audience.

Language: Английский

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Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 158, P. 116871 - 116871

Published: Dec. 5, 2022

Language: Английский

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Electrochemical Detection of a Few Copies of Unamplified SARS-CoV-2 Nucleic Acids by a Self-Actuated Molecular System

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(30), P. 13526 - 13537

Published: July 20, 2022

The existing electrochemical biosensors lack controllable and intelligent merit to modulate the sensing process upon external stimulus, leading challenges in analyzing a few copies of biomarkers unamplified samples. Here, we present self-actuated molecular-electrochemical system that consists tentacle trunk modification on graphene microelectrode. contains probe an label keeps upright orientation, which increases recognition efficiency while decreasing pseudosignal. Once nucleic acids are recognized, tentacles nearby along with labels spontaneously actuated downward, generating responses under square wave voltammetry. Thus, it detects SARS-CoV-2 RNAs within 1 min down 4 80 μL, 2-6 orders magnitude lower than those other assays. Double-blind testing 10-in-1 pooled nasopharyngeal samples yield high overall agreement reverse transcription-polymerase chain reaction results. We fabricate portable prototype based this system, showing great potential for future applications.

Language: Английский

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Biointerface Engineering with Nucleic Acid Materials for Biosensing Applications

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(37)

Published: July 13, 2022

Abstract Molecular recognition at the biointerface plays a critical role in sensing molecular interactions (e.g., DNA hybridization) and extracellular changes, which can directly affect detection performance of biosensors sensitivity, specificity, response dynamics). However, conventional biointerfaces show low efficiency due to limited target accessibility. Engineering regulate orientation, spacing, density surface‐confined probes offer an effective approach improve interfaces. Over last decades, engineering with nucleic acid materials has advanced fundamental understanding hybridization kinetics facilitated design improved biosensing platforms for monitoring cellular activities diagnosing relevant diseases. This review summarizes recent progress acid‐based engineering. The development that be applied specific diagnostic applications is briefly introduced. Then roles acids tailoring properties nanosurfaces, cell surfaces, macroscopic surfaces are discussed their comprehensively highlighted. Finally, future challenges perspectives emerging technologies field presented.

Language: Английский

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