Recent advances in centrifugal microfluidics for point-of-care testing

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.

Language: Английский

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Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104882 - 104882

Published: Jan. 1, 2025

Language: Английский

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3D Printed All-in-One Sample Introduction System for ICP-MS: Integrating Chip-Based Array Monolithic Microextraction, Microvalve Control, and Microflow Nebulizer

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Three-dimensional printing (3DP) technology was applied to fabricate an all-in-one sample introduction system for inductively coupled plasma mass spectrometry (ICP-MS) analysis of ultratrace rare-earth elements (REEs) in cells. The developed 3DP comprised a microfluidic chip integrating cell lysis and monolithic microextraction array, microvalve control unit, microflow total consumption high-efficiency nebulizer (MTHEN). It made full use the advantages preparing user-customized three-dimensional structures. On one hand, integrated consisting mixing zone array columns with skeleton structure UiO-66 printed directly, which are beneficial cells buffer extraction target from lysate, respectively, as well improving transfer. other 3DP-MTHEN customized interface directly connected ICP torch, providing high transport efficiency (81.1%) under low flow rate 6 μL min-1. sensitivity ICP-MS by using representative 4.7-6.0 times higher than that obtained commercial Burgener SC175 (detection limits 0.7-13.3 vs 0.7-52.6 ng L-1). Besides, exhibited lower cost ($13 1550). proposed has been determination REEs MCF-7 simple operation, (500 cells), good precision (2.2-11.9%), detection limit (1.3-8.6 L-1), quantitative recovery (71.9%-119%). merits application potential elemental samples very limited volume, especially

Language: Английский

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Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal–Organic Frameworks-Based Immunobeads

Nano Letters, Journal Year: 2023, Volume and Issue: 23(19), P. 9056 - 9064

Published: Sept. 22, 2023

Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) demonstrated dual functions protein detection bacteria identification: (i) ultrasensitive point-of-care (POC) biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification Gram-negative/positive (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) convenient inexpensive for tests. MAFs possess a large surface area, excellent photostability, quantum yield (∼80%), multiple active sites serving as binding domains (IL-6/PCT) on LFAs. The limit (LOD) IL-6/PCT 0.252/0.333 pg/mL. Rapid appraisal vital to guide use medicines. dual-functional have great potential in tests clinical diagnosis bacterial infections.

Language: Английский

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Automated Microfluidic Nucleic Acid Detection Platform-Integrated RPA-T7-Cas13a for Pathogen Diagnosis

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(17), P. 6940 - 6947

Published: April 21, 2023

There is a growing urgent need for point-of-care testing (POCT) devices that integrate sample pretreatment and nucleic acid detection in rapid, economical, non-labor-intensive way. Here, we have developed an automated, portable system employing microfluidic chips integrating rotary valve-assisted recombinase polymerase amplification (RPA)-T7-Cas13a into one-step detection. The RPA clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a were integrated single-chamber reaction. As validation model, used this method to detect Group B streptococci (GBS) DNA achieved sensitivity of 8 copies/reaction, which 6 times more sensitive than gold-standard chain reactions (PCRs). Dual specific recognition with CRISPR/Cas13a makes our ultraspecific, correct from kinds pathogenic bacteria. For the 16 positive 24 negative clinical GBS samples, assay 100% accuracy compared PCR technique. whole procedure can be automatically completed within 30 min, providing robust, sensitive, accurate molecular diagnostic tool POCT.

Language: Английский

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Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(11), P. 3988 - 4007

Published: Oct. 23, 2023

Point-of-care (POC) detection is getting more and attention in many fields due to its accuracy on-site test property. The CRISPR/Cas12a system endowed with excellent sensitivity, target identification specificity, signal amplification ability biosensing because of unique trans-cleavage ability. As a result, lot research has been made develop CRISPR/Cas12a-based biosensors. In this review, we focused on readout strategies summarized recent sensitivity-improving fluorescence, colorimetric, electrochemical signaling. Then introduced novel portability-improving based lateral flow assays (LFAs), microfluidic chips, simplified instruments, one-pot design. the end, also provide our outlook for future development

Language: Английский

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The recent advances of high-throughput biosensors for rapid detection of foodborne pathogens

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 176, P. 117736 - 117736

Published: May 1, 2024

Language: Английский

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High-throughput identification of meat ingredients in adulterated foods based on centrifugal integrated purification-CRISPR array

Food Chemistry, Journal Year: 2024, Volume and Issue: 443, P. 138507 - 138507

Published: Jan. 21, 2024

Language: Английский

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A roadmap to high-speed polymerase chain reaction (PCR): COVID-19 as a technology accelerator

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 246, P. 115830 - 115830

Published: Nov. 16, 2023

The limit of detection (LOD), speed, and cost crucial COVID-19 diagnostic tools, including lateral flow assays (LFA), enzyme-linked immunosorbent (ELISA), polymerase chain reactions (PCR), have all improved because the financial governmental support for epidemic. most notable improvement in overall efficiency among them has been seen with PCR. Its significance human health increased during pandemic, when it emerged as commonly used approach identifying virus. However, problems complexity, expense, PCR deployment point-of-care settings continues to be difficult. Microfluidic platforms offer a promising solution by enabling development smaller, more affordable, faster systems. In this review, we delve into engineering challenges associated advancement high-speed microfluidic equipment. We introduce criteria that facilitate evaluation comparison factors such LOD, cycling efficiency, multiplexing capacity, considering sample volume, fluidics, reactor geometry materials, well heating/cooling methods. also provide comprehensive list commercially available devices conclude projections discussion regarding current obstacles need addressed order progress further field.

Language: Английский

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Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: May 3, 2023

Herbal medicines and preparations are widely used in healthcare systems globally, but concerns remain about their quality safety. New herbal products constantly being introduced to the market under varying regulatory frameworks, with no global consensus on definition or characterization. These biologically active mixtures sold through complex globalized value chains, which create around contamination profit-driven adulteration. Industry, academia, bodies must collaborate develop innovative strategies for identification authentication of botanicals ensure control. High-throughput sequencing (HTS) has significantly improved our understanding total species diversity within DNA mixtures. The standard concept barcoding evolved over last two decades encompass genomic data more broadly. Recent research metabarcoding focused developing methods quantifying product ingredients, yielding meaningful results a framework. Techniques, such as loop-mediated isothermal amplification (LAMP), barcode-based Recombinase Polymerase Amplification (BAR-RPA), coupled High-Resolution Melting (Bar-HRM), microfluidics-based methods, offer affordable tests detection target species. While capture genome skimming considerably increasing resolution challenging plant clades, ddPCR enables quantification samples could be detect intended unwanted ingredients medicines. Here, we explore latest advances emerging DNA-based technologies opportunities they provide taxa tools evaluating safety dietary supplements

Language: Английский

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