Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 399, P. 134804 - 134804
Published: Oct. 20, 2023
Language: Английский
Nature Reviews Bioengineering, Journal Year: 2025, Volume and Issue: unknown
Published: April 11, 2025
Language: Английский
Citations
0
Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14
Published: Feb. 22, 2023
DNA computing has become the focus of research due to its excellent parallel processing capability, data storage capacity, and low energy consumption characteristics. computational units can be precisely programmed through sequence specificity base pair principle. Then, cascaded integrated form large systems. Among them, strand displacement (DSD) is simplest but most efficient method for constructing The inputs outputs DSD are signal strands that transferred next unit. been used construct logic gates, circuits, artificial neural networks, etc. This review introduced recent development DSD-based systems their applications. Some DSD-related tools issues also discussed.
Language: Английский
Citations
9
ACS Nano, Journal Year: 2023, Volume and Issue: 17(18), P. 18055 - 18061
Published: July 27, 2023
This study demonstrates the implementation of Hamming code using DNA-based nanostructures for error detection and correction in communication systems. The designed DNA conduct logical operations to compute check codes identify correct erroneous data based on fluorescence signals. execution intricate logic requires individuals with specialized training. By interpretation signals generated by nanostructures, binary language can be extracted, effectively protecting security. findings highlight potential as a versatile platform reliable transmission.
Language: Английский
Citations
8
Nature Nanotechnology, Journal Year: 2024, Volume and Issue: 19(3), P. 271 - 272
Published: Feb. 16, 2024
Language: Английский
Citations
3
Published: March 18, 2024
Bio-soft matter droplets formed via liquid-liquid phase separation (LLPS) of biopolymers have been found in living cells. Synthetic LLPS recently employed nanobiotechnology for artificial cell construction, molecular robotics, computing, diagnosis, and therapeutics. Controlling the dynamics bio-soft is essential developing such bio-inspired functional systems because maintain their functions based on temporally controlled biomolecular reactions assemblies. Recently, revealed; however, temporal control has not yet achieved. This paper reports DNA-based (DNA droplets). We demonstrate timing-controlled division DNA droplet-based cells time-delayed triggers regulated by non-equilibrium chemical reactions. also investigated it using a reaction-diffusion model. release order multiple triggers, resulting multistep droplet division, that is, pathway reaction landscape. Finally, we an application cells: computing element to compare concentrations microRNA sequences (called comparators). believe will promote design more dynamic cells/molecular robots sophisticated biomedical applications.
Language: Английский
Citations
3
Small, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 3, 2024
DNA amplifier circuits establish powerful tools to dynamically control molecular assembly for computation, sensing, and biological applications. However, the slow reaction speed remains a major barrier their practical utility. Here, diverse fast termed toehold exchange polymerization (TEP) catalysis (TEC) using exchange-mediated as fundamental mechanism are built. Both TEP TEC with duplex hairpin can respond within minutes nucleic acid inputs high fidelity. In addition, amplify live-cell signals fluorescence imaging target RNA dynamics discriminating different cell lines. Compared existing that involve time scales of hours transducing small signals, exhibit much faster dynamics, simpler design, comparable sensitivity. These features make promising platforms develop programmable devices create sensing processing systems, amenable wide
Language: Английский
Citations
3
IEEE Transactions on Systems Man and Cybernetics Systems, Journal Year: 2023, Volume and Issue: 53(10), P. 6031 - 6042
Published: June 9, 2023
The emergence of DNA strand displacement has prompted the development chaotic synchronization techniques, and previous works mainly focus on study for two three-dimensional systems via displacement. In this article, four-dimensional (4-D) loop controllers are designed using several units, three 4-D is achieved by cascading controllers. It revealed from results Visual DSD that reactions can realize nonlinear systems, our method robustness when one controller does not work. Our will provide reference investigation techniques.
Language: Английский
Citations
7
ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(2), P. 521 - 529
Published: Jan. 27, 2024
Biochemical reaction networks can exhibit plastic adaptation to alter their functions in response environmental changes. This capability is derived from the structure and dynamics of functionality biomolecule. biochemical systems essentially related memory learning capabilities, which have been studied DNA computing applications for past decade. However, designing with capabilities using dynamic properties reactions remains challenging. In this study, we propose a basic system design that acquires classical conditioning, phenomenon underlying learning, as typical task. Our based on simple mechanism five strand displacement two degradative reactions. The proposed circuit acquire or lose new function under specific conditions, depending input history formed by repetitive stimuli, exploiting induced different timings.
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(47), P. 18645 - 18654
Published: Nov. 14, 2024
We present a novel activity-based detection strategy for matrix metalloproteinase 2 (MMP2), critical cancer protease biomarker, leveraging mechanism responsive to the proteolytic activity of MMP2 and its integration with CRISPR-Cas12a-assisted signal amplification. designed chemical translator comprising two functional units─a peptide nucleic acid (PNA), fused together. The presents substrate MMP2, while PNA serves as output subsequent processing. This was immobilized on micrometer magnetic beads physical support an assay. incorporated into our design single-stranded DNA partially hybridized sequence bearing region complementary RNA guide CRISPR-Cas12a. target-induced nuclease Cas12a results in degradation FRET-labeled reporters amplified fluorescence signal, enabling low picomolar range, showing limit 72 pg/mL. study provides new principles broader applicability CRISPR-Cas-based biosensing.
Language: Английский
Citations
2
ACS Central Science, Journal Year: 2024, Volume and Issue: 10(12), P. 2285 - 2293
Published: Dec. 11, 2024
DNA computing leverages molecular reactions to achieve diverse information processing functions. Recently developed origami registers, which could be integrated with circuits, allow signal transmission between these enabling circuits perform complex tasks in a sequential manner, thereby enhancing the programming space and compatibility various biomolecules of computing. However, registers support only single-write operations, transfer involves cumbersome time-consuming register movements, limiting speed Here, we designed solid-state that compresses output data from 3D solution 2D surface, establishing rewritable suitable for storage. We heterogeneous integration architecture liquid-state reducing register-mediated time less than 1 h, achieving fast Furthermore, trace amplifier read surface-stored signals back into solution. This compact approach not enhances but also lays foundation visual debugging automated execution algorithms.
Language: Английский
Citations
2