Frontiers in Chemical Biology,
Journal Year:
2024,
Volume and Issue:
3
Published: Aug. 19, 2024
Bioluminescence
(BL)
is
an
amazing
optical
readout
that
has
great
potential
to
be
utilized
in
various
bioassays
and
molecular
imaging,
but
also
some
unique
defects
practical
applications.
The
recent
innovative
research
on
BL
enriched
the
available
repertories
of
toolbox.
While
these
efforts
greatly
diversified
users’
choices
applications,
wide
contrary
do
not
promise
their
successful
applications
bioassays.
This
mainly
due
complexity-driven
confusion
with
diversity
lack
accurate
knowledge
advantages
disadvantages
BL.
review
intended
showcase
BL,
serve
as
a
searchlight
find
directions
for
future
studies.
We
hope
this
provides
instant
references
readers
leads
them
properly
understand
“bright”
“dark”
sides
narrow
down
ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(10), P. 4472 - 4481
Published: Aug. 29, 2024
Acceptor–donor–acceptor
(A-D-A)
structured
π-conjugated
molecules
emerge
as
a
new
class
of
phototheranostic
materials.
The
π-bridge
has
rarely
been
investigated
for
structure
engineering
compared
with
the
D
and
A
units.
This
work
reveals
that
changing
ethylene
into
butadiene
can
efficiently
extend
absorption
wavelength
promote
nonradiative
transition.
Specifically,
not
only
extends
π-conjugation
length
but
also
disrupts
S···O
interlocking,
which
facilitates
intramolecular
torsions
rotations
enhances
charge
transfer
molecule
π-bridge.
As
result,
very
broad
70
nm
red-shift
is
observed,
power
conversion
efficiency
(PCE)
BTVIC
nanoparticles
reaches
82.6%.
Ultimately,
photoacoustic
imaging
(PAI)-guided
photothermal
therapy
(PTT)
applications
in
vivo
demonstrate
deep
penetration
depth
over
7
mm
an
efficient
anti-tumor
effect.
illustrates
modifying
practical
strategy
regulating
A-D-A
interactions
developing
more
agents.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(7), P. 333 - 333
Published: July 8, 2024
Bioanalysis
based
on
optical
imaging
has
gained
significant
progress
in
the
last
few
decades.
Luminescence
probes
are
capable
of
detecting,
monitoring,
and
tracing
particular
biomolecules
complex
biological
systems
to
figure
out
roles
these
molecules
organisms.
Considering
rapid
development
luminescence
for
bio-applications
their
promising
future,
we
have
attempted
explore
working
principles
recent
advances
probes,
hope
helping
readers
gain
a
detailed
understanding
developed
years.
In
this
review,
first
focus
current
widely
used
including
fluorescence
bioluminescence
chemiluminescence
afterglow
photoacoustic
Cerenkov
probes.
The
each
type
probe
concisely
described
bio-application
is
summarized
by
category,
metal
ions
detection,
secretion
imaging,
therapy.
RSC Chemical Biology,
Journal Year:
2024,
Volume and Issue:
5(9), P. 877 - 883
Published: Jan. 1, 2024
The
immunoproteasome
(iCP)
can
be
expressed
under
inflammatory
conditions,
such
as
exposure
to
interferon-gamma
(IFN-γ),
that
alerts
the
cell
begin
generating
iCP
preferentially
over
standard
proteasome
(sCP).
With
becoming
a
widely
targeted
isoform
in
variety
of
diseases,
there
is
need
understand
its
activity
and
expression
cells
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 23, 2024
Abstract
Electrochemiluminescence
(ECL)
microscopy
has
emerged
as
a
powerful
technique
for
single‐cell
imaging
owing
to
its
unparalleled
background‐free
advantages.
However,
controlled
intracellular
ECL
remains
challenging.
Here,
we
developed
stimuli‐responsive
self‐assembled
DNA
nanomachine
that
enables
the
of
target
biomolecules
in
single
cells.
The
nanoprobe
consists
an
nanoemitter
constructed
from
Ru(bpy)
3
2+
‐doped
metal–organic
framework
nanoreactor
core,
with
polymer
hydrogel
(DNAgel)
shell
acting
stimuli‐gated
layer.
outer
functionalized
DNAgel
was
specifically
designed
block
generation
and
dissociate
by
ATP
molecules,
thereby
enabling
selective
recovery
emission
capability.
Such
engineered
successfully
achieved
targeted
distribution
spatial
resolution.
In
addition,
various
should
be
generalizable
simply
changing
switching
sequence
probe.
Our
research
provided
reliable
strategy
within
cells,
which
will
broaden
application
single‐molecule
profiling.
Chemical Communications,
Journal Year:
2023,
Volume and Issue:
59(55), P. 8552 - 8555
Published: Jan. 1, 2023
Firefly
luciferin
methyl
ester
is
hydrolyzed
by
monoacylglycerol
lipase
MAGL,
amidase
FAAH,
poorly-characterized
hydrolase
ABHD11,
and
hydrolases
known
for
ACS Chemical Biology,
Journal Year:
2024,
Volume and Issue:
19(5), P. 1035 - 1039
Published: May 8, 2024
Red-shifted
bioluminescence
is
highly
desirable
for
diagnostic
and
imaging
applications.
Herein,
we
report
a
semisynthetic
NanoLuc
(sNLuc)
based
on
complementation
of
split
NLuc
(LgBiT)
with
synthetic
peptide
(SmBiT)
functionalized
fluorophore
BRET
emission.
We
observed
exceptional
ratios
diverse
fluorophores,
notably
in
the
red
(I674/I450
>
14),
brightness
that
sufficient
naked
eye
detection
blood
or
through
tissues.
To
exemplify
its
utility,
LgBiT
was
fused
to
miniprotein
binds
HER2
(affibody,
ZHER2),
selective
HER2+
SK-BR-3
cells
over
HER2–
HeLa
demonstrated.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(13), P. 7338 - 7338
Published: July 4, 2024
Bioluminescence
imaging
(BLI)
is
an
indispensable
technique
for
visualizing
the
dynamics
of
diverse
biological
processes
in
mammalian
animal
models,
including
cancer,
viral
infections,
and
immune
responses.
However,
a
critical
scientific
challenge
remains:
non-invasively
homeostatic
disease
mechanisms
freely
moving
animals
to
understand
molecular
basis
exercises,
social
behavior,
other
phenomena.
Classical
BLI
relies
on
prolonged
camera
exposure
accumulate
limited
number
photons
that
traveled
from
deep
tissues
anesthetized
or
constrained
animals.
Recent
advancements
synthetic
bioluminescence
reactions,
utilizing
artificial
luciferin-luciferase
pairs,
have
considerably
increased
detectable
tissues,
facilitating
high-speed
capture
objects.
In
this
review,
I
provide
overview
emerging
reactions
enable
non-invasive
This
approach
holds
potential
uncover
unique
physiological
are
inaccessible
with
current
methodologies.
