bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 30, 2024
Abstract
Super-resolution
imaging
based
on
the
localization
of
single
emitters
requires
a
spatio-temporal
control
ON
and
OFF
state.
To
this
end,
photoactivatable
fluorophores
are
adapted
as
they
can
be
turned
upon
light
irradiation.
Here
we
present
concept
called
Self-Triggered
Photooxidation
Cascade
(STPC)
photooxidation
plasma
membrane
targeted
leuco-rhodamine
(LRhod-PM),
non-fluorescent
reduced
form
rhodamine
probe.
Upon
visible
irradiation
small
number
oxidized
rhodamines,
Rhod-PM,
acts
photosensitizer
to
generate
singlet
oxygen
capable
oxidize
state
LRhod-PM.
We
showed
that
phenomenon
is
kinetically
favored
by
high
local
concentration
propagates
quickly
when
probe
embedded
in
bilayers.
In
addition,
close
proximity
dyes
favors
photobleaching.
At
single-molecule
level,
concomitant
activation/bleaching
phenomena
allow
reaching
blinking
regime
enabling
microscopy
for
super-resolution
live
cellular
membranes.
Advanced Optical Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 24, 2024
Abstract
Photomodulable
fluorophores
constitute
advanced
materials
as
they
possess
the
ability
to
modify
their
photophysical
properties
upon
photoirradiation.
A
new
mechanism
of
photoconversion
is
recently
established,
called
Directed
Photooxidation
Induced
Conversion
based
on
coupling
with
Aromatic
Singlet
oxygen
Reactive
Moieties
(ASORMs).
In
this
work,
The
(DPIC)
intended
be
applied
Perylene
BisImide
(PBI)
due
its
appealing
properties.
experimental
results
showed
that
two
ASORMs
PBI
core,
here
furan
and
pyrrole,
led
impressive
photomodulable
fluorophores.
While
PBI‐F
exhibited
a
100
nm
shift,
PBI‐P
displayed
an
80‐fold
fluorescence
intensity
enhancement
photoactivation.
Analysis
photoproducts
conversion
do
not
involve
addition
singlet
ASORM.
Instead,
occurred
through
efficient
successive
photocyclizations.
Finally,
intracellular
vesicles
are
successfully
photoconverted
by
means
endocytosed
PLGA‐polymer
nanoparticles
loaded
PBI‐F.
This
study
highlights
unique
capability
furan‐
pyrrole‐conjugated
enable
optical
phototransformation
The Journal of Physical Chemistry Letters,
Journal Year:
2024,
Volume and Issue:
15(36), P. 9247 - 9254
Published: Sept. 3, 2024
Rationally
designed
molecules
with
versatile
conformations
are
ideal
candidates
for
creating
challenging
single
component-based
multicolor
emissive
materials.
Herein,
a
new
strategy
is
presented
by
introducing
C–C
double
bond
in
an
o-carborane
derivative.
Compared
to
linear
connection
using
triple
(CbPyY),
(CbPyE)
exhibited
zigzag
structure
and
unique
fluorescence
behavior.
Yellow,
yellowish
orange,
red
crystals
or
films
of
CbPyE
can
be
obtained,
while
only
orange
ones
CbPyY
were
achieved
under
the
same
condition.
Single
crystal
XRD
theoretical
calculation
studies
revealed
brings
asymmetry
one
arm
CbPyE,
which
causes
conformation
diversity
leads
emission.
Interestingly,
different
emissions
showed
responses
acetone
vapor;
yellow
much
higher
sensitivity
faster
response
than
other
two.
It
believed
that
prepared
materials
proposed
would
contribute
future
advances
single-fluorophore-based
Journal of Materials Chemistry C,
Journal Year:
2023,
Volume and Issue:
11(31), P. 10642 - 10650
Published: Jan. 1, 2023
Macrocyclic
tetra(naphthimidazole)resorcin[4]arenes
exhibit
large
Stokes
shifts
upon
embedding
in
PMMA
matrix
enabling
their
applications
luminescent
solar
concentrator
technology.
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
136(19)
Published: April 3, 2024
Abstract
Der
Einsatz
kleiner
fluoreszierender
Moleküle
spielt
in
der
Medizin
und
Arzneimittelentwicklung
eine
immer
größere
Rolle.
Mit
diesem
Beitrag
ergänzen
wir
das
Portfolio
leistungsstarker
Fluorophore
durch
die
zufällige
Entdeckung
Entwicklung
einer
neuen
Klasse
mit
einem
Imidazo[1,2‐
a
]pyridiniumtriflat‐Kern,
„PyrAt‐Verbindungen“
nennen.
Diese
werden
einzigen
Schritt
aus
leicht
verfügbaren
Materialien
synthetisiert
(>60
Beispiele)
zeigen
Stokes‐Verschiebungen
bis
zu
240
nm,
während
sie
gleichzeitig
NIR−I‐Emissionen
bei
λ
max
720
nm
erreichen.
Computergestützte
Studien
ermöglichen
Plattform
für
Vorhersage
von
Em
.
Darüber
hinaus
demonstrieren
Kompatibilität
dieser
neuartigen
Lebendzellbildgebung
HEK293‐Zellen,
was
darauf
hindeutet,
dass
potente
intrazelluläre
Marker
sind.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 30, 2024
Abstract
Super-resolution
imaging
based
on
the
localization
of
single
emitters
requires
a
spatio-temporal
control
ON
and
OFF
state.
To
this
end,
photoactivatable
fluorophores
are
adapted
as
they
can
be
turned
upon
light
irradiation.
Here
we
present
concept
called
Self-Triggered
Photooxidation
Cascade
(STPC)
photooxidation
plasma
membrane
targeted
leuco-rhodamine
(LRhod-PM),
non-fluorescent
reduced
form
rhodamine
probe.
Upon
visible
irradiation
small
number
oxidized
rhodamines,
Rhod-PM,
acts
photosensitizer
to
generate
singlet
oxygen
capable
oxidize
state
LRhod-PM.
We
showed
that
phenomenon
is
kinetically
favored
by
high
local
concentration
propagates
quickly
when
probe
embedded
in
bilayers.
In
addition,
close
proximity
dyes
favors
photobleaching.
At
single-molecule
level,
concomitant
activation/bleaching
phenomena
allow
reaching
blinking
regime
enabling
microscopy
for
super-resolution
live
cellular
membranes.
