Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 10, 2025
The
sensitive
detection
of
the
radioactive
thorium
(Th)
ion
with
an
oxidation
state
+4
(Th4+)
is
great
significance
for
environmental
protection
and
life
safety.
In
this
study,
five
fluorescence
sensors
regulated
donor-acceptor
(D-A)
interactions
were
constructed
Th4+
based
on
intramolecular
charge
transfer
aggregation-induced
emission
mechanisms.
Among
developed
sensors,
TPE-D
bearing
electron-deficient
π-bridge
weak
D-A
presented
ratiometric
behavior
toward
in
aqueous
solution
due
to
its
characteristics
unique
D-A-D
structures.
Moreover,
showed
excellent
selectivity
sensitivity
detection,
limit
was
as
low
8.1
×
10-8
M.
sensing
mechanism
observation
revealed
that
could
coordinate
hydroxyl,
imine,
carbonyl
groups
accompanied
by
electron
process.
addition,
selectively
be
enriched
lysosome.
Both
lysosome
liver
mice
zebrafish
realized
strategy,
a
mobile-assisted
approach
actual
water
samples
also
established
high
sensitivity.
This
first
report
organelles
organs,
which
provides
reliable
strategy
radionuclide
toxicology
analysis
applications.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(8), P. 3436 - 3444
Published: Feb. 19, 2024
Cerebral
ischemia–reperfusion
injury
(CIRI),
a
cause
of
cerebral
dysfunction
during
infarction
treatment,
is
closely
associated
with
mitochondrial
viscosity
and
hydrogen
peroxide
(H2O2).
However,
the
accurate
measurement
H2O2
levels
in
CIRI
challenging
because
lack
sufficient
selectivity
blood–brain
barrier
(BBB)
penetration
existing
monitoring
tools
related
to
CIRI,
hampering
exploration
role
CIRI.
To
address
this
issue,
we
designed
an
activatable
fluorescent
probe,
mitochondria-targeting
styryl-quinolin-ium
(Mito-IQS),
excellent
properties
including
high
selectivity,
targeting,
BBB
penetration,
for
visualization
brain.
Based
on
real-time
capabilities
bursts
were
visualized
This
probe
can
be
used
monitor
therapeutic
effects
butylphthalein
treatment.
More
importantly,
vivo
experiments
further
confirmed
that
was
levels.
discovery
provides
new
insights
study
expected
accelerate
process
diagnosis,
drug
design.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(20), P. 11242 - 11347
Published: Oct. 9, 2024
Biopsy,
including
tissue
and
liquid
biopsy,
offers
comprehensive
real-time
physiological
pathological
information
for
disease
detection,
diagnosis,
monitoring.
Fluorescent
probes
are
frequently
selected
to
obtain
adequate
on
processes
in
a
rapid
minimally
invasive
manner
based
their
advantages
biopsy.
However,
conventional
fluorescent
have
been
found
show
aggregation-caused
quenching
(ACQ)
properties,
impeding
greater
progresses
this
area.
Since
the
discovery
of
aggregation-induced
emission
luminogen
(AIEgen)
promoted
advancements
molecular
bionanomaterials
owing
unique
high
quantum
yield
(QY)
signal-to-noise
ratio
(SNR),
Angewandte Chemie International Edition,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 9, 2025
Abstract
Adenosine
triphosphate
(ATP),
the
primary
energy
currency
in
cells,
is
dynamically
regulated
across
different
subcellular
compartments.
The
ATP
interplay
between
mitochondria
and
endoplasmic
reticulum
(ER)
underscores
their
coordinated
roles
various
biochemical
processes,
highlighting
necessity
for
precise
profiling
of
dynamics.
Here
we
present
an
exogenously
endogenously
dual‐regulated
DNA
nanodevice
spatiotemporally
selective,
subcellular‐compartment
specific
signal
amplification
sensing.
system
allows
exogenous
NIR
light‐controlled
spatiotemporal
localization
activation
aptamer
sensor
or
ER,
while
a
endogenous
enzyme
organelles
further
drives
via
consumption
molecular
beacon
fuels,
resulting
significantly
enhanced
sensitivity
spatial
precision
organelle
interest.
Furthermore,
demonstrate
application
this
robust
monitoring
fluctuations
ER
following
drug
interventions.
This
advancement
provides
powerful
tool
improving
our
understanding
cellular
energetics
at
level
holds
potential
development
targeted
therapeutics.
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 4, 2025
Liver
disease
poses
a
significant
challenge
to
global
health,
and
its
early
diagnosis
is
crucial
for
improving
treatment
outcomes
patient
prognosis.
Since
fluctuation
of
key
biomarkers
during
the
onset
progression
liver
diseases
can
directly
reflect
health
normal/abnormal
function,
biomarker-based
assays
are
vital
tools
detection
disease.
In
this
context,
small
molecule
fluorescent
probes
have
undeniably
emerged
as
indispensable
analysis,
with
an
ever-growing
number
molecule-based
being
developed
over
recent
years,
sole
aim
monitoring
relevant
This
perspective
will
focus
on
development
application
primarily
last
10
years
diagnosing
range
disease-related
processes.
It
outline
foundational
design
strategies
developing
promising
probes,
their
optical
response
biomarkers,
how
they
been
demonstrated
in
proof-of-concept
imaging
applications.
Current
challenges
new
developments
field
be
discussed,
providing
insights
highlighting
opportunities
field.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(14), P. 5428 - 5436
Published: March 29, 2024
Rheumatoid
arthritis
(RA),
a
common
chronic
inflammatory
illness,
is
still
incurable,
reducing
the
sufferers'
quality
of
life
significantly.
Adenosine
5′-triphosphate
(ATP)
and
hypochlorous
acid
(HOCl)
are
key
indicators
in
RA,
but
their
precise
mechanisms
RA
pathophysiology
unknown.
As
result,
order
to
detect
ATP
HOCl
simultaneously,
we
created
two
new
dual-channel/localization
single-molecule
fluorescence
probes,
RhTNMB
RhFNMB.
Furthermore,
RhFNMB
outperformed
terms
detection
performance.
produce
independent
responses
light
red
channel
(λex
=
520
nm,
λem
586
nm)
deep
620
688
nm),
respectively,
without
spectral
crosstalk.
It
should
be
noted
that
probe
successfully
imaged
mitochondria
cells.
Surprisingly,
demonstrated
remarkable
ability
diagnosis
treatment
Pseudomonas
aeruginosa-induced
abdominal
inflammation
mice.
We
continued
apply
track
discovered
concentrations
were
considerably
greater
joints
than
normal
joints.
also
confirmed
therapeutic
effect
methotrexate
on
RA.
This
study
first
achieve
dual-channel
imaging
HOCl,
which
great
value
for
early
therapy
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Jan. 2, 2025
Bioorthogonal
chemistry-mediated
self-assembly
holds
great
promise
for
dynamic
molecular
imaging
in
living
organisms.
However,
existing
approaches
are
limited
to
nanoaggregates
with
'always-on'
signals,
suffering
from
high
signal-to-background
ratio
(SBR)
and
compromised
detection
sensitivity.
Herein
we
report
a
nitrile-aminothiol
(NAT)
bioorthogonal
fluorogenic
probe
(CyNAP-SS-FK)
ultrasensitive
diagnosis
of
orthotopic
hepatocellular
carcinoma.
This
comprises
nitrile-substituted
hemicyanine
scaffold
cysteine
tail
dually
locked
biomarker-responsive
moieties.
Upon
dual
cleavage
by
tumor-specific
cathepsin
B
biothiols,
the
1,2-aminothiol
residue
is
exposed
spontaneously
reacts
nitrile
group
situ
intramolecular
macrocyclization,
enabling
near-infrared
fluorescence
(NIRF)
turn-on
as
well
self-assembly.
In
male
mice,
such
'cleavage-click-assembly'
regimen
allows
real-time
small
cancerous
lesions
(~2
mm
diameter)
improved
SBR
(~5)
extended
window
(~36
h),
outperforming
conventional
clinical
assays.
study
not
only
presents
NAT
click
reaction-based
probes
but
also
highlights
generic
dual-locked
design
these
probes.
Existing
can
suffer
low
Here,
authors
sensing
action
lesions.
