Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

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Detecting mitochondrial hypochlorous acid and viscosity in atherosclerosis models via NIR fluorescent probes

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 156, P. 108191 - 108191

Published: Jan. 20, 2025

Language: Английский

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Assessing Atherosclerosis by Super-Resolution Imaging of HClO in Foam Cells Using a Ratiometric Fluorescent Probe

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(35), P. 14215 - 14221

Published: Aug. 20, 2024

Atherosclerosis (AS) is the leading cause of cardiovascular disease. Foam cells, with elevated lipid droplets (LDs) and HClO levels, are main components atherosclerotic plaques that characteristic AS. Super-resolution imaging can be used to visualize distribution LDs in foam cells at nanometer level, facilitating identification HClO. In present study, we report development a ratiometric fluorescent probe,

Language: Английский

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Cell-free biosensing array via Avi-tag oriented immobilization for allergen-specific IgE detection

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 427, P. 137185 - 137185

Published: Jan. 5, 2025

Language: Английский

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Silver Mediated Electrochemical Sensor for Rapid Determination of Hypochlorite in the Coexistence of ROS

Published: Jan. 1, 2025

Rapid, sensitive and selective quantification of hypochlorite (ClO-) is highly important to understand its critical role in environmental disinfection various physiological processes. However, the ClO- complex matrices often interfered by other coexisting reactive oxygen species (ROS), therefore, it desirable design a new sensing strategy distinguish from oxidative speices real samples. An electrochemical sensor (ITO-AuNPs-cys-Ag) was constructed this work, where Au nanoparticles (AuNPs) serve as substrate Ag+ acts signal probe, interconnected via cysteamine (cys) through Au-S Ag-N bonds. The disruption coordination bond causes detachment interface, resulting decrease signal. A wide linear range 0.75-25 μM low detection limit (LOD) 0.27 were obtained with sensor. Further study revealed that plays preventing interferences ROS samples based on appropriate redox potential reversible cycle Ag2+/Ag+, thus as-prepared can be directly applied for determination environment ferroptosis NIH3T3 cells. results present work not only provide useful exploration reaction processes pathways ClO-, but also demonstrate improve selectivity sensors.

Language: Английский

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Endogenous HClO-Gated Cascade MicroRNA Imaging for Precise Diagnosis of Atherosclerosis In Vivo

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Precise imaging of atherosclerotic plaques using biomarkers could prompt the diagnosis and clinical management atherosclerosis (AS)-driven cardiovascular diseases. MicroRNA-155 (miR-155) plays a critical role in AS development, with its expression notably upregulated foam cells within plaques. However, miRNA methods for face significant challenges, including low specificity, inefficient delivery, poor cell selectivity. Herein, we develop an endogenous hypochlorous acid (HClO)-gated cascade signal amplification strategy precise miR-155 living cells, enabling accurate vivo ex detection This utilizes phosphorothioate (PT)-modified hairpin probe that is specifically deprotected by HClO uncaged miR-155, triggering catalytic assembly (CHA) to amplify fluorescence signals. The PT-CHA probes are encapsulated lipid nanoparticles (LNs), followed conjugating phosphatidylserine (PS)-binding peptide (PBP) selectively targeting intensity PT-CHA@LN-PBP aorta region shows clear differentiation among AS-bearing mice, miR-155-/- healthy mice. Moreover, strongly correlates plaque area progression can discriminate vulnerability risk under curve (AUC) 0.94. Imaging human aortic tissues further validates probe's capacity distinguish from normal endarterium. These findings establish as noninvasive, reliable diagnostic tool assessment AS.

Language: Английский

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Global research landscape on nanotechnology in acute lung injury: a bibliometric analysis

Frontiers in Digital Health, Journal Year: 2025, Volume and Issue: 7

Published: March 4, 2025

Background Acute lung injury is a common respiratory emergency that seriously affects the life, health and quality of life patients, especially after global COVID-19 pneumonia. The application nanotechnology in acute promising. In response to knowledge explosion resulting from rapid publication growth, we applied bibliometric analysis explore research profile thematic trends field. Methods Articles reviews related 2004 2023 were searched. Java-based Citespace, VOSviewer, R software-based Bibiometrix used systematically evaluate publications by spatiotemporal distribution, author subject categories, topic references, keywords. Results A total 1,347 included. number papers has grown exponentially over past 20 years. China was most productive country out all 53 countries, followed United States. Chinese Academy Sciences institution with 76 papers. PARTICLE AND FIBRE TOXICOLOGY journal. top five high-frequency keywords inflammation, oxidative stress, toxicity, vitro , respiratory-distress-syndrome. And emerging delivery, covid-19, extracellular vesicles, therapy, sars-cov-2. Drug delivery are focus current research. Two areas represented development trends: novel nanocarriers higher efficiency lower biotoxicity, other impact nanomaterials progression injury. Conclusion field been period last three Delivery,targeted exosm have this trends:novel biotoxicity such as exosomes solid lipid nanoparticles,

Language: Английский

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Self-Adaptive Release of Stem Cell-Derived Exosomes from a Multifunctional Hydrogel for Accelerating MRSA-Infected Diabetic Wound Repair

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: 147(19), P. 16362 - 16378

Published: April 30, 2025

Chronic diabetic wounds are prone to severe skin necrosis and bacterial infections, with elevated reactive oxygen species (ROS) persistent inflammation further hindering the healing process. Developing smart dressings multifunctional therapeutic capabilities simultaneously combat reduce oxidative stress, alleviate inflammation, promote tissue regeneration remains a significant challenge. Here, we introduce self-adaptive yet hydrogel (Exo-Gel) designed accelerate methicillin-resistant Staphylococcus aureus (MRSA)-infected wound repair. Exo-Gel utilizes choline phosphate (CP) groups both anchor stem cell-derived exosomes (Exo) via electrostatic interactions disrupt membranes, providing inherent bacteriostatic effects. Additionally, ROS-responsive thioketal (TK) linkers enable release of based on local ROS levels while also scavenging excess ROS. This synergistic system facilitates by modulating reducing promoting M2 macrophage polarization, enhancing cell proliferation, myofibroblast migration, angiogenesis, collagen deposition regeneration. In Sprague-Dawley rats MRSA-infected full-thickness wounds, achieved remarkable activity accelerated healing. offers cost-effective, multifunctional, strategy for managing chronic requiring no external components or operations, making it highly practical translatable clinical applications.

Language: Английский

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Extracellular Vesicles in Atherosclerosis: From Pathogenesis to Theranostic Applications

Small, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Abstract Atherosclerosis is a chronic vascular inflammatory disorder, and the cardiovascular disease resulting from it leading cause of death worldwide. Extracellular vesicles (EVs) are small membrane secreted by cells, playing crucial role in regulating intercellular communication, signal transduction, various pathophysiological processes. EVs have been shown to play significant progression regression atherosclerotic disease. Therefore, comprehensive understanding pathogenesis atherosclerosis essential for advancing knowledge this field driving development innovation novel diagnostic therapeutic strategies. This review summarizes pathological roles atherosclerosis, including their involvement endothelial cell inflammation, phenotypic transformation smooth muscle macrophage activation, foam formation, plaque thrombosis rupture. Furthermore, discusses innovative EV‐based strategies diagnosing treating atherosclerosis. Finally, challenges prospects translational research on discussed. work aims provide an EV‐centered perspective diagnosis treatment

Language: Английский

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HClO‐Activated Near‐Infrared Chemiluminescent Probes with a Malononitrile Group for In‐Vivo Imaging

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Chemiluminescence (CL) imaging has emerged as a powerful approach to molecular that allows exceptional sensitivity with virtually no background interference because of its unique capacity emit photons without an external excitation source. Despite high potential, the application this nascent technique faces challenges current chemiluminescent agents have limited reactive sites, require complex synthesis, are insufficiently bright, and lack near-infrared emission. Herein, series HClO-activated probes exhibit robust emission studied. Specifically engineered respond HClO, known biomarker acute inflammation, these achieve high-contrast in vivo by eliminating need for constant excitation. Comprehensive experimental theoretical investigations demonstrate CL depends on reactivity vinylene bonds, following concerted decomposition oxidized molecule. The nanoparticles facilitates providing real-time, visualization inflammatory conditions. This advancement signifies leap forward nanoplatforms biomedical expands available methodologies field.

Language: Английский

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