Rational design of an ultrabright quinolinium-fused rhodamine turn-on fluorescent probe for highly sensitive detection of SO2 derivatives: applications in food safety and bioimaging

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136291 - 136291

Published: Oct. 24, 2024

Language: Английский

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Enzyme-responsive, multi-lock optical probes for molecular imaging and disease theranostics

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Enzyme-responsive, multi-lock optical probes exhibit superior detection specificity towards certain biomolecular events and pathological conditions, offering high disease diagnostic accuracy treatment efficacy.

Language: Английский

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Precision Molecular Engineering of Compact Near-Infrared Fluorophores

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Organic fluorophores with near-infrared (NIR) emission and reduced molecular size are crucial for advancing bioimaging biosensing technologies. Traditional methods, such as conjugation expansion heteroatom engineering, often fail to reduce fluorophore without sacrificing NIR properties. Addressing this challenge, our study utilized quantum chemical calculations structure-property relationship analysis establish an iterative design approach enable precision engineering compact, single-benzene-based fluorophores. These newly developed exhibit emissions up 759 nm maintain weights low 192 g/mol, approximately 50% of that Cy7. Additionally, they display unique environmental sensitivity─nonemissive in aqueous solutions but highly emissive lipid environments. This property significantly enhances their utility wash-free imaging live cells. Our findings mark a substantial breakthrough paving the way more efficient adaptable methodologies.

Language: Английский

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Dual-Locked Fluorescence Probe for Monitoring the Dynamic Transition of Pulmonary Macrophages

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Pulmonary macrophages undergo dynamic changes in population, proportion, and polarization during respiratory diseases. Monitoring these is critical for understanding their roles pathology, improving the diagnosis, guiding drug development. However, current analytic methods based on tissue biopsy are invasive static, limiting ability to provide such information. Herein, we report a dual-locked macrophage-specific renal-clearable probe (DMRPNOCas) monitoring of pulmonary influenza A virus (IAV) infection. DMRPNOCas activates fluorescence presence two biomarkers (caspase-1 NO) only coexpressed by M1 macrophages. To optimize NO reactivity, scaffold screened from hemicyanine derivatives with an o-phenylenediamine group positioned differently indole ring. Notably, para-substituted demonstrates higher NO-activated compared its meta-substituted counterpart. This enhancement, as revealed quantum chemical calculations, attributed differential inhibition twisted intramolecular charge transfer induced reaction. specifically distinguishes other leukocytes including T cells, neutrophils, M2 macrophages, capability unmatched single-locked control probes reported probes. Consequently, enables vivo uncovering extensive recruitment monocyte-derived within 48 h IAV process accompanied significant reduction alveolar These findings new insights into macrophage-mediated inflammation underscore potential precise diagnosis pathological processes.

Language: Английский

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“Crossbreeding” NIR‐II flavchromene for PSMA‐positive prostate cancer detection and image‐guided surgery

Smart Molecules, Journal Year: 2024, Volume and Issue: 2(3)

Published: July 4, 2024

Abstract Prostate‐specific membrane antigen (PSMA) is known to be overexpressed in prostate cancer (PCa). The development of precise and rapid imaging technologies monitor PSMA crucial for early diagnosis therapy. Fluorescence the second near‐infrared window (NIR‐II) has emerged as a powerful tool real‐time tracking vivo visualization, offering high sensitivity resolution. However, there lack stable, bright easy‐to‐implement NIR‐II fluorescent probes targeting. Herein, we presented PSMA‐targeting probe FC‐PSMA based on π‐conjugated crossbreeding dyed strategy that affords stability, large extinction coefficient, good brightness. As demonstrated, displayed fluorescence quantum yield fetal bovine serum (FBS). Following intravenous injection FC‐PSMA, tumor‐to‐normal ratio intensity steadily increased over time, reaching peak at 48 h (tumor‐to‐leg = 12.16 ± 0.90). This advancement enables identification PC through imaging, facilitating high‐performance guidance resection surgery.

Language: Английский

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Dual-Locked Enzyme-Activatable Fluorescence Probes for Precise Bioimaging

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Real-time visualization of endogenous enzymes not only helps reveal the underlying biological principles but also provides pathological information for cancer/disease diagnosis and even treatment guidance. To this end, enzyme-activatable fluorescence probes are frequently fabricated that turn their signals "on" exclusively at enzyme-rich region, thus enabling noninvasive real-time imaging interest molecular level with superior sensitivity selectivity. However, in a complex context, commonly used single (i.e., single-locked) may suffer from "false positive" healthy tissues be insufficient to accurately indicate occurrence certain diseases. Therefore, dual-locked have been promoted address these issues. Using dual (or an enzyme another stimulus) as "keys", they permit simultaneous detection distinct biomarkers, offering significantly enhanced precision toward events. Considering recent reviews on remain scarce, we provide review. We summarize progress, particularly highlighting breakthroughs last three years, discuss challenges field.

Language: Английский

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Long-Term and Real-Time Post-External Radiotherapy Assessment Based on an In Situ Activatable Radiolabeled Platform

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Long-term monitoring in postoperative assessment is essential for clinicians to assess the effectiveness of therapies and establish subsequent clinical pharmacotherapeutic plans. However, precise real-time often overlooked, relying instead on various histopathological cytological assays or experience physicians. Therefore, it urgent develop a general strategy long-term, real-time, accurate assessment. Herein, we present facile method utilizing radiolabeled probes postradiotherapy The probe consists tumor-specific targeting group, an external radiotherapy-activated peptide sequence (DEVD), 177Lu-1,4,7,10-tetraazacyclododecane-N,N′,N″,N‴-tetraacetic acid (DOTA)-decorated tetraphenyl ethylene. This design not only avoids photobleaching limitations associated with traditional organic ligands long-term but also achieves situ aggregation at lesion site, allowing prolonged tumor retention over 96 h. work serves as glance assessment, broadening possibilities design, application, translation radionuclide-labeled probes.

Language: Английский

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Architecture design of improved near-infrared hemicyanine dyes for biosensing and bioimaging

Chinese Science Bulletin (Chinese Version), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Dual-Locked Near-Infrared Fluorescent Probe for Real-Time Imaging of Hydrogen Sulfide/Matrix Metallopeptidase-2 In Vivo

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Hydrogen sulfide (H2S) and matrix metallopeptidase 2 (MMP-2) are inextricably linked in the occurrence development of diseases treatment diseases. However, most activatable imaging probes currently developed single-locked that do not simultaneously detect H2S MMP-2 levels at disease sites severely hinder real-time accurate analysis dynamic relationship between two interrelated biomarkers. Herein, we report a dual-locked H2S/MMP-2-activatable near-infrared (NIR) fluorescent probe through dual-Förster resonance energy transfer (FRET) mechanism specifically detects tumors or acute lung injury (ALI) establishes level expression. Initially, fluorescence is turned off due to from methylene blue (MB) both cationic electrochromic material (dicationic 1,1,4,4-tetraarylbutadiene, EM 12+) quencher QSY21. Upon reaction with H2S/MMP-2 ALI, NIR activated, enabling ALI. Additionally, this precisely tracks effects exogenous glucocorticoids for ALI on expression, providing powerful molecular tool early prediction outcomes

Language: Английский

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Logic-gated approach for targeted delivery and site-selective activation of photothermal agents in precision cancer treatment

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Logic-gated strategies represent a promising approach to achieving highly selective cancer therapies. In this work, we present LG-AB (Logic-Gated Aza-BODIPY), an OFF-ON photothermal therapy (PTT) agent designed selectively target cells. undergoes red-shift in its maximum absorbance wavelength when activated the tumor microenvironment, enabling molecule precisely generate heat cancerous tissue upon light irradiation. Unlike conventional activatable agents that rely on single biomarker, employs AND logic-gated design, where glucose transporter 1 (GLUT1) overexpression facilitates targeted cellular uptake cells, followed by activation through elevated glutathione (GSH) levels. Beyond demonstrating efficacy human lung and murine breast show effectively attenuates progression heat-induced apoptosis, with minimal off-target effects surrounding tissues. The versatility of strategy is further demonstrated development application LG-CPT Camptothecin), which utilizes same design. Our results enhancing specificity limiting collateral damage can be broadly applied across different therapeutic agents.

Language: Английский

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