Structural Insights into the N–N Bond-Formation Mechanism of the Heme-Dependent Piperazate Synthase KtzT

ACS Catalysis, Journal Year: 2025, Volume and Issue: 15(2), P. 1265 - 1273

Published: Jan. 7, 2025

N–N bond formation plays a critical role in the synthesis of organic compounds and has broad applications producing dyes, pharmaceuticals, functional materials. However, is challenging due to nucleophilicity nitrogen. Here, we determined crystal structures heme-dependent enzyme, KtzT, which catalyzes cyclization l-N5-hydroxyornithine (l-N5-OH-Orn) yield l-piperazate (l-piz) by linking two intramolecular nitrogen atoms. The complex structure KtzTC197A with l-N5-OH-Orn reveals substrate-interaction network, validated through mutagenesis experiments. Notably, N5 atom substrate directly coordinates heme iron, precluding oxygen binding. This supports prior knowledge that KtzT an oxygen-independent reaction. Intriguingly, exhibits distinct conformations our crystals. Based on distance between atoms product accommodation pose KtzTC197A/l-piz structure, conformation 2 likely productive pose, while more extended 1 may be transient state facilitating entry into catalytic tunnel. A potential pathway also proposed. These findings offer structural insights for developing bio- metal-catalyzed methods formation.

Language: Английский

---

Application of a fluorescent probe exhibiting a large stokes shift after the precise detection of hydrazine in plants, foods and living cells

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137452 - 137452

Published: Feb. 3, 2025

Language: Английский

---

Facile Design of Hollow Cube-Like Cu-Doped Co3o4 Derived from a Co-Based Prussian Blue Analogue Via Kirkendall Effect for Hydrazine Monitoring

Published: Jan. 1, 2025

Language: Английский

---

Recent Developments and Challenges in the Enzymatic Formation of Nitrogen–Nitrogen Bonds

ACS Catalysis, Journal Year: 2024, Volume and Issue: 15(1), P. 310 - 342

Published: Dec. 17, 2024

The biological formation of nitrogen–nitrogen (N–N) bonds represents intriguing reactions that have attracted much attention in the past decade. This interest has led to an increasing number N–N bond-containing natural products (NPs) and related enzymes catalyze their (referred this review as NNzymes) being elucidated studied greater detail. While more detailed information on biosynthesis NPs, which only become available recent years, provides unprecedented source biosynthetic enzymes, potential for biocatalytic applications been minimally explored. With review, we aim not provide a comprehensive overview both characterized NNzymes hypothetical biocatalysts with putative bond forming activity, but also highlight from perspective. We present compare conventional synthetic approaches linear cyclic hydrazines, hydrazides, diazo- nitroso-groups, triazenes, triazoles allow comparison enzymatic routes via these functional groups. Moreover, pathways well diversity reaction mechanisms are presented according direct groups currently accessible enzymes.

Language: Английский

---

Hot off the press

Natural Product Reports, Journal Year: 2024, Volume and Issue: 41(8), P. 1214 - 1218

Published: Jan. 1, 2024

A personal selection of 32 recent papers is presented covering various aspects current developments in bioorganic chemistry and novel natural products such as nitidane from

Language: Английский

---

Abiotic anammox by a naturally occurring mineral

Nature Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Language: Английский

---