Quantum Dot–Peptide–Fullerene Bioconjugates for Visualization of in Vitro and in Vivo Cellular Membrane Potential DOI
Okhil K. Nag, Michael H. Stewart, Jeffrey R. Deschamps

et al.

ACS Nano, Journal Year: 2017, Volume and Issue: 11(6), P. 5598 - 5613

Published: May 17, 2017

We report the development of a quantum dot (QD)–peptide–fullerene (C60) electron transfer (ET)-based nanobioconjugate for visualization membrane potential in living cells. The bioconjugate is composed (1) central QD donor, (2) membrane-inserting peptidyl linker, and (3) C60 acceptor. photoexcited donor engages ET with acceptor, resulting quenching photoluminescence (PL) that tracks positively number moieties arrayed around QD. nature QD-capping ligand also modulates efficiency; neutral coating facilitates greater than negatively charged carboxylated ligand. Steady-state photophysical characterization confirms an ET-driven process between donor–acceptor pair. When introduced to cells, amphiphilic QD–peptide–C60 labels plasma by insertion peptide–C60 portion into hydrophobic bilayer, while hydrophilic sits on exofacial side membrane. Depolarization cellular augments process, which manifested as further PL. demonstrate HeLa PC12 primary cortical neurons significant PL (ΔF/F0 2–20% depending QD–C60 separation distance) response depolarization KCl. Further, we show ability use probe combination conventional voltage-sensitive dyes (VSDs) simultaneous two-channel imaging potential. In vivo electrical stimulation, optical optimal configuration exhibits temporal responsivity stimulation similar VSDs. Notably, however, construct displays 20- 40-fold ΔF/F0 tractable system offers advantages ease assembly, large ΔF/F0, enhanced photostability, high throughput without need complicated organic synthesis or genetic engineering, respectively, required traditional VSDs fluorescent protein constructs.

Language: Английский

Fluorogenic Probes for Multicolor Imaging in Living Cells DOI
Gražvydas Lukinavičius, Luc Reymond,

Keitaro Umezawa

et al.

Journal of the American Chemical Society, Journal Year: 2016, Volume and Issue: 138(30), P. 9365 - 9368

Published: July 15, 2016

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 715 nm, respectively. SiR700-based probes F-actin, microtubules, lysosomes, SNAP-tag are fluorogenic, cell-permeable, compatible with superresolution microscopy. In conjunction based on the previously introduced carboxy-SiR650, permit microscopy in thus significantly expanding our capacity imaging living cells.

Language: Английский

Citations

259
Activity‐Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond DOI Creative Commons
Kevin J. Bruemmer, Steven W. M. Crossley, Christopher J. Chang

et al.

Angewandte Chemie International Edition, Journal Year: 2019, Volume and Issue: 59(33), P. 13734 - 13762

Published: Oct. 12, 2019

Emerging from the origins of supramolecular chemistry and development selective chemical receptors that rely on lock-and-key binding, activity-based sensing (ABS)-which utilizes molecular reactivity rather than recognition for analyte detection-has rapidly grown into a distinct field to investigate production regulation species mediate biological signaling stress pathways, particularly metal ions small molecules. Chemical reactions exploit diverse enable sensitive synthetic methods decipher their contributions within complex living environments. The broad utility this reaction-driven approach facilitates application imaging platforms ranging fluorescence, luminescence, photoacoustic, magnetic resonance, positron emission tomography modalities. ABS are also being expanded other fields, such as drug materials discovery.

Language: Английский

Citations

233
Teaching Old Dyes New Tricks: Biological Probes Built from Fluoresceins and Rhodamines DOI Open Access
Luke D. Lavis

Annual Review of Biochemistry, Journal Year: 2017, Volume and Issue: 86(1), P. 825 - 843

Published: April 12, 2017

Small-molecule fluorophores, such as fluorescein and rhodamine derivatives, are critical tools in modern biochemical biological research. The field of chemical dyes is old; colored molecules were first discovered the 1800s, scaffolds have been known for over a century. Nevertheless, there has renaissance using these to create biochemistry biology. application chemistry, biochemistry, molecular genetics, optical physics old structures enables drives development novel, sophisticated fluorescent dyes. This review focuses on an important example biology-the melding new knowledge-leading useful advanced experiments.

Language: Английский

Citations

231
Caveat fluorophore: an insiders’ guide to small-molecule fluorescent labels DOI
Jonathan B. Grimm, Luke D. Lavis

Nature Methods, Journal Year: 2021, Volume and Issue: 19(2), P. 149 - 158

Published: Dec. 23, 2021

Language: Английский

Citations

206
Xanthene-Based NIR-II Dyes for In Vivo Dynamic Imaging of Blood Circulation DOI

Diankai Liu,

Zixu He, Yanyan Zhao

et al.

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(41), P. 17136 - 17143

Published: Oct. 11, 2021

Fluorescence bioimaging through the second near-infrared window (NIR-II, 1000–1700 nm) has attracted much attention due to its deep penetration and high contrast. However, exploring new fluorescent materials, especially small molecular fluorophores with long wavelength brightness, is still quite challenging. By expanding π-conjugation enhancing intramolecular charge transfer effect, herein we report a series of xanthene-based NIR-II dyes, named VIXs. Among these VIX-4 exhibits best performance fluorescence emission at 1210 nm brightness been used for dynamically imaging blood flow mice 200 fps. virtue spatiotemporal resolution dynamic imaging, can distinguish directly artery vein direction measure volume by videos. This study provides not only an effective tool spatial temporal but also promising conjugated skeleton dyes.

Language: Английский

Citations

153
Design and Application of Fluorescent Probes to Detect Cellular Physical Microenvironments DOI

Junbao Ma,

Rui Sun,

Kaifu Xia

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(4), P. 1738 - 1861

Published: Feb. 14, 2024

The microenvironment is indispensable for functionality of various biomacromolecules, subcellular compartments, living cells, and organisms. In particular, physical properties within the biological could exert profound effects on both cellular physiology pathology, with parameters including polarity, viscosity, pH, other relevant factors. There a significant demand to directly visualize quantitatively measure fluctuation in spatiotemporal resolution. To satisfy this need, analytical methods based fluorescence probes offer great opportunities due facile, sensitive, dynamic detection that these molecules enable varying settings from vitro samples live animal models. Herein, we focus types small molecule fluorescent measurement microenvironment, mechanical force, temperature, electron potential. For each parameter, primarily describe chemical mechanisms underlying how are correlated changes signals. This review provides an overview perspective development environment, expand knowledge process, enrich diagnostic tools human diseases.

Language: Английский

Citations

106
Metabolically driven maturation of human-induced-pluripotent-stem-cell-derived cardiac microtissues on microfluidic chips DOI
Nathaniel Huebsch,

Bérénice Charrez,

Gabriel Neiman

et al.

Nature Biomedical Engineering, Journal Year: 2022, Volume and Issue: 6(4), P. 372 - 388

Published: April 27, 2022

Language: Английский

Citations

76
Multimodal control of dendritic cell functions by nociceptors DOI
Pavel Hanč,

Rodrigo J. Gonzalez,

Irina B. Mazo

et al.

Science, Journal Year: 2023, Volume and Issue: 379(6639)

Published: March 31, 2023

It is known that interactions between nociceptors and dendritic cells (DCs) can modulate immune responses in barrier tissues. However, our understanding of the underlying communication frameworks remains rudimentary. Here, we show control DCs three molecularly distinct ways. First, release calcitonin gene-related peptide imparts a transcriptional profile on steady-state characterized by expression pro-interleukin-1β other genes implicated DC sentinel functions. Second, nociceptor activation induces contact-dependent calcium fluxes membrane depolarization enhances their production proinflammatory cytokines when stimulated. Finally, nociceptor-derived chemokine CCL2 contributes to orchestration DC-dependent local inflammation induction adaptive against skin-acquired antigens. Thus, combined actions chemokines, neuropeptides, electrical activity fine-tune

Language: Английский

Citations

63
Video-based pooled screening yields improved far-red genetically encoded voltage indicators DOI
He Tian,

Hunter C. Davis,

J. David Wong-Campos

et al.

Nature Methods, Journal Year: 2023, Volume and Issue: 20(7), P. 1082 - 1094

Published: Jan. 9, 2023

Language: Английский

Citations

52
Silicon-RosIndolizine fluorophores with shortwave infrared absorption and emission profiles enable in vivo fluorescence imaging DOI
William E. Meador, Eric Y. Lin, Irene Lim

et al.

Nature Chemistry, Journal Year: 2024, Volume and Issue: 16(6), P. 970 - 978

Published: March 25, 2024

Language: Английский

Citations

28