DNA origami

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Jan. 28, 2021

Language: Английский

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Building machines with DNA molecules

Nature Reviews Genetics, Journal Year: 2019, Volume and Issue: 21(1), P. 5 - 26

Published: Oct. 21, 2019

Language: Английский

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Functionalizing Framework Nucleic‐Acid‐Based Nanostructures for Biomedical Application

Advanced Materials, Journal Year: 2021, Volume and Issue: 34(46)

Published: Nov. 17, 2021

Strategies for functionalizing diverse tetrahedral framework nucleic acids (tFNAs) have been extensively explored since the first successful fabrication of tFNA by Turberfield. One-pot annealing at least four DNA single strands is most common method to prepare tFNA, as it optimizes cost, yield, and speed assembly. Herein, focus on key merits tFNAs their potential biomedical applications. The natural ability scavenge reactive oxygen species, along with remarkable enhancement in cellular endocytosis tissue permeability based its appropriate size geometry, promotes cell-material interactions direct or probe cell behavior, especially treat inflammatory degenerative diseases. Moreover, structural programmability enables development static tFNA-based nanomaterials via engineering functional oligonucleotides therapeutic molecules, dynamic attachment stimuli-responsive apparatuses, leading applications targeted therapies, regeneration, antitumor strategies, antibacterial treatment. Although there are impressive performance significant progress, challenges prospects nanostructures still indicated this review.

Language: Английский

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DNA nanostructures coordinate gene silencing in mature plants

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(15), P. 7543 - 7548

Published: March 25, 2019

Significance Plant bioengineering will be necessary to sustain plant biology and agriculture, where the delivery of biomolecules such as DNA, RNA, or proteins cells is at crux biotechnology. Here, we show that DNA nanostructures can internalize into deliver siRNA mature tissues without external aid. Furthermore, demonstrate nanostructure size, shape, compactness, stiffness affect both internalization subsequent gene silencing efficiency. Interestingly, also find attachment locus affects endogenous pathway. Our work demonstrates programmable plants details figures merit for future implementation in agriculture.

Language: Английский

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Framework nucleic acids as programmable carrier for transdermal drug delivery

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: March 8, 2019

DNA nanostructures are promising drug carriers with their intrinsic biocompatibility, uniformity and versatility. However, rapid serum disintegration leads to low bioavailability at targeted sites following systemic administration, hindering biomedical applications. Here we demonstrate transdermal delivery of framework nucleic acids (FNAs) through topical By designing FNAs distinct shapes sizes, interrogate penetration on mice human skin explant. Skin histology reveals size-dependent penetration, ≤75 nm effectively reaching dermis layer. 17 nm-tetrahedral show greatest 350 µm from periphery. Importantly, structural integrity is maintained during the penetration. Employing a mouse melanoma model, application doxorubicin-loaded accommodates ≥2-fold improvement in accumulation tumor inhibition relative topically-applied free doxorubicin, or doxorubicin loaded liposomes polymeric nanoparticles. Programmable minimal biodistribution underlines FNA potential as localized carriers.

Language: Английский

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Modulation of the Cellular Uptake of DNA Origami through Control over Mass and Shape

Nano Letters, Journal Year: 2018, Volume and Issue: 18(6), P. 3557 - 3564

Published: May 14, 2018

Designer nanoparticles with controlled shapes and sizes are increasingly popular vehicles for therapeutic delivery due to their enhanced cell-delivery performance. However, our ability fashion has offered only limited control over these parameters. Structural DNA nanotechnology an unparalleled self-assemble three-dimensional nanostructures near-atomic resolution features, thus, it offers attractive platform the systematic exploration of parameter space relevant nanoparticle uptake by living cells. In this study, we examined cell a panel 11 distinct DNA-origami shapes, largest dimension ranging from 50–400 nm, in 3 different lines. We found that larger particles greater compactness were preferentially internalized compared elongated, high-aspect-ratio particles. Uptake kinetics also be more cell-type-dependent than shape-dependent, specialized endocytosing dendritic cells failing saturate 12 h study. The knowledge gained current study furthers understanding how particle shape affects cellular heralds development nanotechnologies toward improvement state-of-the-art vehicles.

Language: Английский

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DNA Nanostructure-Programmed Like-Charge Attraction at the Cell-Membrane Interface

ACS Central Science, Journal Year: 2018, Volume and Issue: 4(10), P. 1344 - 1351

Published: Sept. 25, 2018

Cell entry of anionic nano-objects has been observed in various types viruses and self-assembled DNA nanostructures. Nevertheless, the physical mechanism underlying internalization these particles across negatively charged cell membrane remains poorly understood. Here, we report use virus-mimicking designer nanostructures with near-atomic resolution to program "like-charge attraction" at interface cytoplasmic membranes. Single-particle tracking shows that cellular tetrahedral (TDNs) depends primarily on lipid-raft-mediated pathway, where caveolin plays a key role providing short-range attraction interface. Both simulation experimental data establish TDNs approach their corners minimize electrostatic repulsion, they induce uneven charge redistribution under short-distance confinement by caveolin. We expect nanoscale like-charge provides new clues for viral general rules rational design carriers therapeutics.

Language: Английский

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Rationally Designed DNA‐Origami Nanomaterials for Drug Delivery In Vivo

Advanced Materials, Journal Year: 2018, Volume and Issue: 31(45)

Published: Oct. 4, 2018

Abstract The recent decades have seen a surge of new nanomaterials designed for efficient drug delivery. DNA nanotechnology has been developed to construct sophisticated 3D nanostructures and artificial molecular devices that can be operated at the nanoscale, giving rise variety programmable functions fascinating applications. In particular, DNA‐origami feature rationally geometries precise spatial addressability, as well marked biocompatibility, thus providing promising candidate Here, successful efforts employ self‐assembled drug‐delivery vehicles are summarized. remaining challenges open opportunities also discussed.

Language: Английский

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Uptake and Fate of Fluorescently Labeled DNA Nanostructures in Cellular Environments: A Cautionary Tale

ACS Central Science, Journal Year: 2019, Volume and Issue: 5(5), P. 882 - 891

Published: April 26, 2019

Fluorescent dye labeling of DNA oligonucleotides and nanostructures is one the most used techniques to track their fate cellular localization inside cells. Here, we report that intracellular fluorescence, even FRET signals, cannot be correlated with uptake intact structures. Live cell imaging revealed high colocalization cyanine-labeled oligos phosphorylated small-molecule cyanine dyes, degradation products from these compounds. Nuclease strands outside results in a misleading fluorescent signal. The signal saturated by fluorescence product (phosphorylated dye). To test our hypothesis, synthesized range structures, including Cy3- Cy5-labeled cubes tetrahedra, different stabilities toward nucleases. All give signals within mitochondria after strongly colocalize free control. Kinetics experiments stable structures delayed. We also studied several parameters influencing data: stability strand, fixation methods can wash away signal, position on design experiments. hold tremendous potential for biomedical applications biotechnology because biocompatibility, programmability, easy synthesis. However, few examples successful machines vivo have been reported. believe this contribution as guide better when using order further propel biological development, application nanostructures.

Language: Английский

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Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(7), P. 3976 - 4050

Published: March 29, 2023

DNA nanotechnology is a unique field, where physics, chemistry, biology, mathematics, engineering, and materials science can elegantly converge. Since the original proposal of Nadrian Seeman, significant advances have been achieved in past four decades. During this glory time, origami technique developed by Paul Rothemund further pushed field forward with vigorous momentum, fostering plethora concepts, models, methodologies, applications that were not thought before. This review focuses on recent progress origami-engineered nanomaterials five years, outlining exciting achievements as well unexplored research avenues. We believe spirit assets Seeman left for scientists will continue to bring interdisciplinary innovations useful next decade.

Language: Английский

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