Nuclear receptors in podocyte biology and glomerular disease

Nature Reviews Nephrology, Journal Year: 2020, Volume and Issue: 17(3), P. 185 - 204

Published: Sept. 17, 2020

Language: Английский

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Interactions Among Non-Coding RNAs in Diabetic Nephropathy

Frontiers in Pharmacology, Journal Year: 2020, Volume and Issue: 11

Published: March 3, 2020

Diabetic Nephropathy (DN) is the most common cause of End-stage renal disease (ESRD). Although various treatments and diagnosis applications are available, DN remains a clinical economic burden. Recent findings showed that noncoding RNAs (ncRNAs) play an important role in progression, potentially can be used as biomarkers therapeutic targets. NcRNAs refers to RNA species do not encode for any protein, known ncRNAs microRNAs (miRNAs), long (lncRNAs) circular (circRNAs). Dysregulation these was reported before patients animal models DN. Importantly, there some interactions between regulate crucial steps progression. Here, we aimed discuss their with critical genes Elucidating regulatory network will allow better understanding molecular mechanisms how they act new also potential targets treatment.

Language: Английский

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miR‐218 regulates diabetic nephropathy via targeting IKK‐β and modulating NK‐κB‐mediated inflammation

Journal of Cellular Physiology, Journal Year: 2019, Volume and Issue: 235(4), P. 3362 - 3371

Published: Sept. 24, 2019

Abstract Diabetic nephropathy (DN) is a common clinically relevant complication of diabetes that associated with damage to the capillaries, yet etiology this condition remains unclear. Nuclear factor‐kappa B (NF‐κB) activation known be DN‐related inflammation and disease progression. Recent work indicated microRNAs are diagnostic biomarkers DN progression inflammation in DN. miR‐218 play key regulatory roles certain cancers humans, while its influence on pathology uncertain. The present study, therefore, sought assess how influences both rat streptozotocin‐induced model as well an vitro system which mouse podocytes were stimulated high glucose levels. We found markedly downregulated systems relative appropriate controls, downregulation was IKK‐β upregulation. In model, overexpressing sufficient reduce renal injury. further determined podocyte proliferation impaired by treatment, leading apoptotic death these cells, mimics able phenotypes. Overexpressing also significantly dampened inflammatory responses system, evidenced reduced tumor necrosis factor‐α, interleukin‐6 (IL‐6), IL‐1β, MCP‐1 then confirmed targeting messenger RNA encoding using dual‐luciferase reporter assay. Together, our results provide clear evidence regulate NF‐κB‐mediated inflammation, central

Language: Английский

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Involvement of miR‐27a‐3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress

Journal of Cellular Physiology, Journal Year: 2020, Volume and Issue: 236(2), P. 1454 - 1468

Published: July 21, 2020

Diabetic nephropathy (DN) is acknowledged as a serious chronic complication of diabetes mellitus. Nevertheless, its pathogenesis complicated and unclear. Thus, in this study, the role miR-27a-3p-prohibitin/TMBIM6 signaling axis progression DN was elucidated. Type 2 diabetic db/db mice high glucose (HG)-challenged HK-2 cells were used vivo vitro models. Our results showed that miR-27a-3p upregulated prohibitin or transmembrane BAX inhibitor motif containing 6 (TMBIM6) downregulated kidney tissues HG-treated cells. Silencing enhanced expression TMBIM6 Inhibition improved functional injury, evidenced by decreased blood glucose, urinary albumin, serum creatinine, urea nitrogen levels. MiR-27a-3p silencing ameliorated renal fibrosis, reflected reduced profibrogenic genes (e.g., transforming growth factor β1, fibronectin, collagen I III, α-smooth muscle actin). Furthermore, inhibition relieved mitochondrial dysfunction mice, including upregulation membrane potential, complex III activities, adenosine triphosphate, cytochrome C, well suppressing reactive oxygen species production. In addition, attenuated endoplasmic reticulum (ER) stress, p-IRE1α, p-eIF2α, XBP1s, CHOP. Mechanically, we identified direct targets miR-27a-3p. protected from apoptosis, extracellular matrix accumulation, dysfunction, ER stress regulating TMBIM6. Taken together, reveal regulates DN, which can be potential therapeutic target.

Language: Английский

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The role of microRNAs in diseases and related signaling pathways

Molecular Biology Reports, Journal Year: 2021, Volume and Issue: 49(7), P. 6789 - 6801

Published: Oct. 31, 2021

Language: Английский

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