Plant Secondary Metabolite Biosynthesis and Transcriptional Regulation in Response to Biotic and Abiotic Stress Conditions

Agronomy, Journal Year: 2021, Volume and Issue: 11(5), P. 968 - 968

Published: May 13, 2021

Plant secondary metabolites (SMs) play important roles in plant survival and creating ecological connections between other species. In addition to providing a variety of valuable natural products, help protect plants against pathogenic attacks environmental stresses. Given their sessile nature, must themselves from such situations through accumulation these bioactive compounds. Indeed, act as herbivore deterrents, barriers pathogen invasion, mitigators oxidative stress. The SMs are highly dependent on factors light, temperature, soil water, fertility, salinity. For most plants, change an individual factor can alter the content even if remain constant. this review, we focus how affect during both biotic abiotic stress conditions. Furthermore, discuss application elicitors culture systems well stimulating effects metabolites. Specifically, shikimate pathway aromatic amino acids produced pathway, which precursors range including terpenoids, alkaloids, sulfur- nitrogen-containing We also detail biosynthesis is altered by several genes related metabolite pathways. Genes responsible for various species conditions regulated transcriptional WRKY, MYB, AP2/ERF, bZIP, bHLH, NAC, discussed here.

Language: Английский

---

BBX16, a B‐box protein, positively regulates light‐induced anthocyanin accumulation by activating MYB10 in red pear

Plant Biotechnology Journal, Journal Year: 2019, Volume and Issue: 17(10), P. 1985 - 1997

Published: April 9, 2019

Summary The red coloration of pear ( Pyrus pyrifolia ) results from anthocyanin accumulation in the fruit peel. Light is required for biosynthesis pear. A homolog Arabidopsis thaliana BBX 22 , Pp 16 was differentially expressed after fruits were removed bags and may be involved biosynthesis. Here, expression function analysed. 's highly induced by white‐light irradiation, as accumulation. ectopic increased hypocotyls tops flower stalks. localized nucleus showed trans‐activity yeast cells. Although could not directly bind to promoter MYB 10 or CHS one‐hybrid assays, complex 16/Pp HY 5 strongly trans‐activated pathway genes tobacco. overexpression calli enhanced during light treatments. Additionally, transient peel accumulation, while virus‐induced gene silencing decreased patterns family members analysed, six additional genes, which light‐induced biosynthesis, identified. Thus, a positive regulator but it induce biosynthesis‐related itself needed gain full function. Our work uncovered regulatory modes suggested potential functions other regulation thereby providing target further studies on

Language: Английский

---

Apple bZIP transcription factor MdbZIP44 regulates abscisic acid‐promoted anthocyanin accumulation

Plant Cell & Environment, Journal Year: 2018, Volume and Issue: 41(11), P. 2678 - 2692

Published: June 25, 2018

Phytohormone abscisic acid (ABA) induces anthocyanin biosynthesis; however, the underlying molecular mechanism is less known. In this study, we found that apple MYB transcription factor MdMYB1 activated biosynthesis in response to ABA. Using a yeast screening technique, isolated MdbZIP44, an ABA-induced bZIP apple, as co-partner with MdMYB1. MdbZIP44 promoted accumulation ABA by enhancing binding of promoters downstream target genes. Furthermore, identified MdBT2, BTB protein, MdbZIP44-interacting protein. A series molecular, biochemical, and genetic analysis suggested MdBT2 degraded protein through Ubiquitin-26S proteasome system, thus inhibiting MdbZIP44-modulated biosynthesis. Taken together, reveal novel working MdbZIP44-mediated

Language: Английский

---

Activator‐type R2R3‐MYB genes induce a repressor‐type R2R3‐MYB gene to balance anthocyanin and proanthocyanidin accumulation

New Phytologist, Journal Year: 2018, Volume and Issue: 221(4), P. 1919 - 1934

Published: Sept. 17, 2018

Summary Anthocyanin and proanthocyanidin ( PA ) accumulation is regulated by both myeloblastosis MYB activators repressors, but little information available on hierarchical interactions between the positive negative regulators. Here, we report a R2R3‐ repressor in peach, designated Pp 18, which acts as regulator of anthocyanin accumulation. 18 can be activated anthocyanin‐ ‐related activators, expressed at fruit ripening juvenile stages when anthocyanins or PAs, respectively, are being synthesized. The protein competes with for binding to basic Helix Loop Helixes bHLH s), develops fine‐tuning regulatory loop balance In addition, motif R3 domain C1 C2 repression motifs C‐terminus confer repressive activity 18. Our study also demonstrates modifying feedback loop, prevents cells from excess s, serves model balancing secondary metabolite transcriptional level.

Language: Английский

---

EIN3-LIKE1, MYB1, and ETHYLENE RESPONSE FACTOR3 Act in a Regulatory Loop That Synergistically Modulates Ethylene Biosynthesis and Anthocyanin Accumulation

PLANT PHYSIOLOGY, Journal Year: 2018, Volume and Issue: 178(2), P. 808 - 823

Published: June 20, 2018

Ethylene regulates climacteric fruit ripening, and EIN3-LIKE1 (EIL1) plays an important role in this process. In apple (Malus domestica), coloration is accompanied by ethylene release during but the molecular mechanism that underlies these two physiological processes unknown. study, we found treatment markedly induced as well expression of MdMYB1, a positive regulator anthocyanin biosynthesis coloration. addition, MdEIL1 directly bound to promoter MdMYB1 transcriptionally activated its expression, which resulted Furthermore, interacted with ETHYLENE RESPONSE FACTOR3, key biosynthesis, thereby providing feedback for regulation. Overall, our findings provide insight into involving synergistic interaction signal transcription factor regulate apple.

Language: Английский

---

Repressors of anthocyanin biosynthesis

New Phytologist, Journal Year: 2021, Volume and Issue: 231(3), P. 933 - 949

Published: April 17, 2021

Anthocyanins play a variety of adaptive roles in both vegetative tissues and reproductive organs plants. The broad functionality these compounds requires sophisticated regulation the anthocyanin biosynthesis pathway to allow proper localization, timing, optimal intensity pigment deposition. While it is well-established that committed steps are activated by highly conserved MYB-bHLH-WDR (MBW) protein complex virtually all flowering plants, repression seems be achieved wide small RNA families function different tissue types response developmental, environmental, hormonal cues. In this review, we survey recent progress identification repressors characterization their molecular mechanisms. We find seemingly very modules act through remarkably similar logic, so-called 'double-negative logic'. Much double-negative production involves signal-induced degradation or sequestration from MBW complex. discuss functional evolutionary advantages logic design compared with simple sequential positive regulation. These provide plausible explanation as why plants have evolved so many repressors.

Language: Английский

---

Transcriptional Factors Regulate Plant Stress Responses Through Mediating Secondary Metabolism

Genes, Journal Year: 2020, Volume and Issue: 11(4), P. 346 - 346

Published: March 25, 2020

Plants are adapted to sense numerous stress stimuli and mount efficient defense responses by directing intricate signaling pathways. They respond undesirable circumstances produce stress-inducible phytochemicals that play indispensable roles in plant immunity. Extensive studies have been made elucidate the underpinnings of defensive molecular mechanisms various species. Transcriptional factors (TFs) involved regulations through acting as mediators perceiving signals downstream gene expression. The cross interactions TFs crosstalk decisive determining accumulation metabolites. Here, we collected major regulating secondary metabolism for direct cessation factors. We focused on six TF families including AP2/ERF, WRKY, bHLH, bZIP, MYB, NAC. This review is compilation where researches were conducted explore contribution metabolites combating influences. Modulation these at transcriptional post-transcriptional levels can facilitate breeding genetic improvement crop plants regarding sensitivity response production compounds.

Language: Английский

---

Role of plant secondary metabolites in defence and transcriptional regulation in response to biotic stress

Plant Stress, Journal Year: 2023, Volume and Issue: 8, P. 100154 - 100154

Published: April 3, 2023

Secondary metabolites in plants have been recognized as a novel basis of potential bio-pesticides, paving the way for their use sustainable agriculture. Plant secondary pivotal roles plant-pathogen interactions. Some important are terpenoids, flavanols, flavones, etc., stress-inducible phytochemicals playing an role plant immune response development. Pathogen enters into host cell, multiply and utilise biological mechanism plants, causing hazard to world food assembly. Under stressed circumstances, evolve powerful intricate system growth defensive action. On other hand, transcription factors (TFs) assist counter adverse environments by acting mediators stress signal regulating stress-responsive gene expression. The transcriptional post-transcriptional manipulation is capable aid molecular breeding genetic modification meant improved metabolite synthesis. Although presence numerous has established life, very slight known about interaction with pathogens specific mechanisms involved leading immunity. Chemical pesticides wreaking havoc on our environment. As consequence, environmental-friendly alternatives disease management, like plant-based metabolites, should be explored. In this appraisal, we reviewed relation pathogens, contribution innate immunity, action, regulation TFs combating infections eco-friendly approach.

Language: Английский

---

The bZip transcription factor HY5 mediates CRY1a‐induced anthocyanin biosynthesis in tomato

Plant Cell & Environment, Journal Year: 2018, Volume and Issue: 41(8), P. 1762 - 1775

Published: March 22, 2018

Abstract The production of anthocyanin is regulated by light and corresponding photoreceptors. In this study, we found that exposure to blue overexpression CRY1a are associated with increased accumulation in tomato ( Solanum lycopersicum L.). These responses the result changes mRNA protein levels SlHY5 , which a transcription factor. vitro vivo experiments using electrophoretic mobility shift assay ChIP‐qPCR assays revealed could directly recognize bind G‐box ACGT‐containing element promoters biosynthesis genes, such as chalcone synthase 1, 2, dihydroflavonol 4‐reductase . Silencing OE‐ lines decreased anthocyanin. findings presented here not only deepened our understanding how controls photoprotection leaves, but also allowed us explore potential targets for improving pigment production.

Language: Английский

---

Solar UV light regulates flavonoid metabolism in apple (Malus x domestica)

Plant Cell & Environment, Journal Year: 2018, Volume and Issue: 41(3), P. 675 - 688

Published: Jan. 10, 2018

Abstract Ultraviolet‐B light (UV‐B) is one environmental signal perceived by plants that affects the flavonoid pathway and influences levels of anthocyanins, flavonols, proanthocyanidins. To understand mechanisms underlying UV exposure, apple trees were grown under spectral filters altered transmission solar light. Fruit analysis showed induced changes in physiology, metabolism, gene expression during development over a season. These sustained after storage. Under low UV, ripening was delayed, fruit size decreased, anthocyanin flavonols reduced. Expression response to for genes regulation biosynthesis flavonols. Transcription flavonol synthase (FLS), ELONGATED HYPOCOTYL 5 (HY5), MYB10, MYB22 down‐regulated throughout reduced UV. Functional testing FLS promoter activated HY5, this enhanced presence MYB22. The can also be regulator, MYB10. As ambient vary around globe, study has implications future crop production, quality which determined

Language: Английский

---