Proceedings of the National Academy of Sciences,
Journal Year:
2010,
Volume and Issue:
107(47), P. 20382 - 20387
Published: Nov. 8, 2010
Newborn
neurons
in
the
dentate
gyrus
of
adult
hippocampus
rely
upon
cAMP
response
element
binding
protein
(CREB)
signaling
for
their
differentiation
into
mature
granule
cells
and
integration
network.
Among
its
many
targets,
transcription
factor
CREB
activates
expression
a
gene
locus
that
produces
two
microRNAs,
miR-132
miR-212.
In
cultured
cortical
hippocampal
neurons,
functions
downstream
from
to
mediate
activity-dependent
dendritic
growth
spine
formation
variety
pathways.
To
investigate
whether
and/or
miR-212
contribute
maturation
dendrites
newborn
hippocampus,
we
inserted
LoxP
sites
surrounding
miR-212/132
specifically
targeted
deletion
by
stereotactically
injecting
retrovirus
expressing
Cre
recombinase.
Deletion
caused
dramatic
decrease
dendrite
length,
arborization,
density.
The
may
express
up
four
distinct
-212
reverse
strands
miR-132*
-212*.
Using
ratiometric
microRNA
sensors,
determined
is
predominantly
active
product
neurons.
We
conclude
required
normal
suggest
this
also
participate
other
examples
CREB-mediated
signaling.
Nucleic Acids Research,
Journal Year:
2012,
Volume and Issue:
40(11), P. 4742 - 4753
Published: Feb. 22, 2012
During
the
last
two
decades,
microRNAs
(miRNAs)
emerged
as
critical
regulators
of
gene
expression.
By
modulating
expression
numerous
target
mRNAs
mainly
at
post-transcriptional
level,
these
small
non-coding
RNAs
have
been
involved
in
most,
if
not
all,
biological
processes
well
pathogenesis
a
number
diseases.
miR-132
and
miR-212
are
tandem
miRNAs
whose
is
necessary
for
proper
development,
maturation
function
neurons
deregulation
associated
with
several
neurological
disorders,
such
Alzheimer's
disease
tauopathies
(neurodegenerative
diseases
resulting
from
pathological
aggregation
tau
protein
human
brain).
Although
their
involvement
neuronal
functions
most
described,
evidences
point
towards
role
many
other
processes,
including
inflammation
immune
functions.
Incidentally,
was
recently
classified
'neurimmiR',
class
operating
within
between
neural
compartments.
In
this
review,
we
propose
an
outline
current
knowledge
about
cells,
by
describing
signalling
pathways
transcription
factors
regulating
putative
or
demonstrated
roles
validated
mRNA
targets.
