Histone post-translational modifications — cause and consequence of genome function

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(9), P. 563 - 580

Published: March 25, 2022

Language: Английский

---

Chromatin organization by an interplay of loop extrusion and compartmental segregation

Proceedings of the National Academy of Sciences, Journal Year: 2018, Volume and Issue: 115(29)

Published: July 2, 2018

Significance Human DNA is 2 m long and folded into a 10-μm-sized cellular nucleus. Experiments have revealed two major features of genome organization: Segregation alternating active inactive regions compartments, formation compacted local domains. These were hypothesized to be formed by different mechanisms: Compartments can microphase separation domains active, motor-driven, loop extrusion. Here, we integrate these mechanisms polymer model show that their interplay coherently explains diverse experimental data for wild-type mutant cells. Our results provide framework the interpretation chromosome organization in phenotypes highlight chromatin complex, matter shaped an phase segregation

Language: Английский

---

Spatial organization of chromatin domains and compartments in single chromosomes

Science, Journal Year: 2016, Volume and Issue: 353(6299), P. 598 - 602

Published: July 22, 2016

Spatial organization inside the nucleus In eukaryotic cells, DNA is packaged into a complex macromolecular structure called chromatin. Wang et al. have developed an imaging method to map position of multiple regions on individual chromosomes, and results confirm that chromatin organized large contact domains TADS (topologically associating domains). Unexpectedly, though, folding deviates from classical fractal-globule model at length scales. Science , this issue p. 598

Language: Английский

---

Visualizing and discovering cellular structures with super-resolution microscopy

Science, Journal Year: 2018, Volume and Issue: 361(6405), P. 880 - 887

Published: Aug. 30, 2018

Super-resolution microscopy has overcome a long-held resolution barrier-the diffraction limit-in light and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved can now be used to image cellular structures three dimensions, multiple colors, living systems with nanometer-scale resolution. These been applied answer questions involving the organization, interaction, stoichiometry, dynamics individual building blocks integration into functional machineries cells tissues. In this Review, we provide an overview methods, state-of-the-art capabilities, constantly expanding applications biology, focus on latter. We will also describe current technical challenges future advances anticipated imaging.

Language: Английский

---

CTCF and cohesin regulate chromatin loop stability with distinct dynamics

eLife, Journal Year: 2017, Volume and Issue: 6

Published: May 3, 2017

Folding of mammalian genomes into spatial domains is critical for gene regulation. The insulator protein CTCF and cohesin control domain location by folding loop structures, which are widely thought to be stable. Combining genomic biochemical approaches we show that co-occupy the same sites physically interact as a biochemically stable complex. However, using single-molecule imaging find binds chromatin much more dynamically than (~1–2 min vs. ~22 residence time). Moreover, after unbinding, quickly rebinds another cognate site unlike search process long (~1 ~33 min). Thus, form rapidly exchanging 'dynamic complex' rather typical Since required formation, our results suggest loops dynamic frequently break reform throughout cell cycle.

Language: Английский

---

The Self-Organizing Genome: Principles of Genome Architecture and Function

Cell, Journal Year: 2020, Volume and Issue: 183(1), P. 28 - 45

Published: Sept. 24, 2020

Language: Английский

---

Deep learning massively accelerates super-resolution localization microscopy

Nature Biotechnology, Journal Year: 2018, Volume and Issue: 36(5), P. 460 - 468

Published: April 16, 2018

Language: Английский

---

Principles of genome folding into topologically associating domains

Science Advances, Journal Year: 2019, Volume and Issue: 5(4)

Published: April 5, 2019

This review discusses the features of TADs across species, and their role in chromosome organization, genome function, evolution.

Language: Английский

---

Ultrastructural Details of Mammalian Chromosome Architecture

Molecular Cell, Journal Year: 2020, Volume and Issue: 78(3), P. 554 - 565.e7

Published: March 25, 2020

Language: Английский

---

Emerging views of the nucleus as a cellular mechanosensor

Nature Cell Biology, Journal Year: 2018, Volume and Issue: 20(4), P. 373 - 381

Published: Feb. 20, 2018

Language: Английский

---