Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes

Nature Communications, Journal Year: 2015, Volume and Issue: 6(1)

Published: May 12, 2015

Abstract Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances FISH-based imaging. We first describe the visualization of single-copy regions genome using single-molecule super-resolution methodologies. then introduce robust reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish maternal paternal homologous chromosomes mammalian insect systems. Both these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which augment with strategy uses secondary oligonucleotides (oligos) produce enhance fluorescent signals. These should substantially expand capability query parent-of-origin-specific chromosome positioning gene expression on cell-by-cell basis.

Language: Английский

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Iterative expansion microscopy

Nature Methods, Journal Year: 2017, Volume and Issue: 14(6), P. 593 - 599

Published: April 17, 2017

Language: Английский

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Polyhedra Self-Assembled from DNA Tripods and Characterized with 3D DNA-PAINT

Science, Journal Year: 2014, Volume and Issue: 344(6179), P. 65 - 69

Published: March 14, 2014

DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report simple, general strategy for one-step of wireframe polyhedra that are more massive most previous structures. A stiff three-arm-junction origami tile motif with precisely controlled angles and arm lengths was used hierarchical assembly experimentally constructed tetrahedron (20 megadaltons), triangular prism (30 cube (40 pentagonal (50 hexagonal (60 megadaltons) edge widths 100 nanometers. The were visualized by means transmission electron microscopy DNA-PAINT super-resolution fluorescent single molecules in solution.

Language: Английский

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Quantitative mapping and minimization of super-resolution optical imaging artifacts

Nature Methods, Journal Year: 2018, Volume and Issue: 15(4), P. 263 - 266

Published: Feb. 19, 2018

Language: Английский

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The Beauty and Utility of DNA Origami

Chem, Journal Year: 2017, Volume and Issue: 2(3), P. 359 - 382

Published: March 1, 2017

Language: Английский

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