Chemistries for DNA Nanotechnology

Chemical Reviews, Journal Year: 2019, Volume and Issue: 119(10), P. 6384 - 6458

Published: Feb. 4, 2019

The predictable nature of DNA interactions enables the programmable assembly highly advanced 2D and 3D structures nanoscale dimensions. access to ever larger more complex has been achieved through decades work on developing structural design principles. Concurrently, an increased focus emerged applications nanostructures. In its nature, is chemically inert nanostructures based unmodified mostly lack function. However, functionality can be obtained chemical modification opportunities are endless. this review, we discuss methodology for functionalization provide examples how being used create functional nanodevices make applicable. We aim encourage researchers adopt modifications as part their in nanotechnology inspire chemists address current challenges within field.

Language: Английский

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Nature‐Inspired Emerging Chiral Liquid Crystal Nanostructures: From Molecular Self‐Assembly to DNA Mesophase and Nanocolloids

Advanced Materials, Journal Year: 2018, Volume and Issue: 32(41)

Published: Aug. 30, 2018

Liquid crystals (LCs) are omnipresent in living matter, whose chirality is an elegant and distinct feature certain plant tissues, the cuticles of crabs, beetles, arthropods, beyond. Taking inspiration from nature, researchers have recently devoted extensive efforts toward developing chiral liquid crystalline materials with self-organized nanostructures exploring their potential applications diverse fields ranging dynamic photonics to energy safety issues. In this review, account on state art emerging nanostructured technological provided. First, overview significance architectures various systems given. Then, recent significant progress different including thermotropic LCs (cholesteric LCs, cubic blue phases, achiral bent-core etc.) lyotropic (DNA nanocellulose graphene oxide LCs) showcased. The review concludes a perspective future scope, opportunities, challenges these truly advanced functional soft promising applications.

Language: Английский

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Architected Origami Materials: How Folding Creates Sophisticated Mechanical Properties

Advanced Materials, Journal Year: 2018, Volume and Issue: 31(5)

Published: Dec. 5, 2018

Abstract Origami, the ancient Japanese art of paper folding, is not only an inspiring technique to create sophisticated shapes, but also a surprisingly powerful method induce nonlinear mechanical properties. Over last decade, advances in crease design, mechanics modeling, and scalable fabrication have fostered rapid emergence architected origami materials. These materials typically consist folded sheets or modules with intricate 3D geometries, feature many unique desirable material properties like auxetics, tunable stiffness, multistability, impact absorption. Rich designs offer great freedom design performance such materials, folding offers opportunity efficiently fabricate these at vastly different sizes. Here, recent studies on aspects materials—geometric analysis, achieved properties, techniques—are highlighted challenges ahead discussed. The synergies between will continue mature flourish this promising field.

Language: Английский

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Visualization of the Cellular Uptake and Trafficking of DNA Origami Nanostructures in Cancer Cells

Journal of the American Chemical Society, Journal Year: 2018, Volume and Issue: 140(7), P. 2478 - 2484

Published: Feb. 6, 2018

DNA origami is a promising molecular delivery system for variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. However, the correlation between DNA-origami design and internalization efficiency in different cell lines remains elusive. We investigated cellular uptake four (DONs) with programmed sizes shapes multiple human lines. The DONs was influenced by size, line. Scavenger receptors were responsible into cells. observed distinct stages process gold nanoparticle (AuNP)-tagged rod-shape DON, using high-resolution transmission electron microscopy. This study provides detailed understanding intracellular trafficking cells, offers new insights future optimization DON-based drug systems treatment.

Language: Английский

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Framework nucleic acids as programmable carrier for transdermal drug delivery

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: March 8, 2019

DNA nanostructures are promising drug carriers with their intrinsic biocompatibility, uniformity and versatility. However, rapid serum disintegration leads to low bioavailability at targeted sites following systemic administration, hindering biomedical applications. Here we demonstrate transdermal delivery of framework nucleic acids (FNAs) through topical By designing FNAs distinct shapes sizes, interrogate penetration on mice human skin explant. Skin histology reveals size-dependent penetration, ≤75 nm effectively reaching dermis layer. 17 nm-tetrahedral show greatest 350 µm from periphery. Importantly, structural integrity is maintained during the penetration. Employing a mouse melanoma model, application doxorubicin-loaded accommodates ≥2-fold improvement in accumulation tumor inhibition relative topically-applied free doxorubicin, or doxorubicin loaded liposomes polymeric nanoparticles. Programmable minimal biodistribution underlines FNA potential as localized carriers.

Language: Английский

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Challenges and Perspectives of DNA Nanostructures in Biomedicine

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(37), P. 15818 - 15833

Published: Feb. 29, 2020

DNA nanotechnology holds substantial promise for future biomedical engineering and the development of novel therapies diagnostic assays. The subnanometer-level addressability nanostructures allows their precise tailored modification with numerous chemical biological entities, which makes them fit to serve as accurate tools multifunctional carriers targeted drug delivery. absolute control over shape, size, function enables fabrication dynamic devices, such nanorobots that can execute programmed tasks react various external stimuli. Even though several studies have demonstrated successful operation both in vitro vivo, major obstacles remain on path real-world applications DNA-based nanomedicine. Here, we summarize current status field main implementations nanostructures. In particular, focus open challenges untackled issues discuss possible solutions.

Language: Английский

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Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

Advanced Materials, Journal Year: 2018, Volume and Issue: 30(24)

Published: Feb. 1, 2018

Abstract DNA encodes the genetic information; recently, it has also become a key player in material science. Given specific Watson–Crick base‐pairing interactions between only four types of nucleotides, well‐designed self‐assembly can be programmable and predictable. Stem‐loops, sticky ends, Holliday junctions, tiles, lattices are typical motifs for forming DNA‐based structures. The oligonucleotides experience thermal annealing near‐neutral buffer containing divalent cation (usually Mg 2+ ) to produce variety nanostructures. These structures not show beautiful landscape, but endowed with multifaceted functionalities. This Review begins fundamental characterization evolutionary trajectory artificial structures, concentrates on their biomedical applications. coverage spans from controlled drug delivery high therapeutic profile accurate diagnosis. A materials, including aptamers, hydrogels, origamis, tetrahedrons, widely utilized different fields. In addition, achieve better performance functionality, hybridization is witnessed, nanostructure modification discussed. Although there impressive advances expectations, development structures/technologies still hindered by several commonly recognized challenges, such as nuclease instability, lack pharmacokinetics data, relatively synthesis cost.

Language: Английский

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Regulatory landscape of nanotechnology and nanoplastics from a global perspective

Regulatory Toxicology and Pharmacology, Journal Year: 2021, Volume and Issue: 122, P. 104885 - 104885

Published: Feb. 21, 2021

Nanotechnology and more particularly nanotechnology-based products materials have provided a huge potential for novel solutions to many of the current challenges society is facing. However, nanotechnology also an area product innovation that sometimes developing faster than regulatory frameworks. This due high complexity some nanomaterials, lack globally harmonised definition different scopes regulation at global level. Research organisations bodies spent efforts in last two decades cope with these challenges. Although there has been significant advancement related analytical approaches labelling purposes as well development suitable test guidelines nanomaterials their safety assessment, still need greater collaboration consensus field. Furthermore, growing societal concerns on plastic litter tiny debris produced by degradation littered objects, impact micro- nanoplastics humans environment emerging issue. Despite increasing research initial discussions nanoplastics, are knowledge gaps thus urgent action. As can be classified specific type incidental future scientific investigations should take into account existing profound nanotechnology/nanomaterials when discussing issues around nanoplastics. review was conceived 2019 Global Summit Regulatory Sciences took place Stresa, Italy, 24–26 September (GSRS 2019) which co-organised Coalition Science (GCRSR) European Commission's (EC) Joint Centre (JRC). The GCRSR consists from various countries globe including EU bodies. excellent platform exchange latest information activities carried out focus application agriculture/food sector, nanomedicines, taking stock promoting further collaboration. Recently, topic become new GCRSR. Besides needs, directions how tools methodologies improve science were elaborated summarising portion during summit. It revealed uncertainties regard physicochemical properties, environmental behaviour toxicological effects, especially testing described dossiers often done early process, material final may behave differently. harmonisation quantification risk assessment micro/nanoplastics, documentation studies sharing databases highlighted important aspects look at.

Language: Английский

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DNA Origami Route for Nanophotonics

ACS Photonics, Journal Year: 2018, Volume and Issue: 5(4), P. 1151 - 1163

Published: Feb. 12, 2018

The specificity and simplicity of the Watson–Crick base pair interactions make DNA one most versatile construction materials for creating nanoscale structures devices. Among several DNA-based approaches, origami technique excels in programmable self-assembly complex, arbitrary shaped with dimensions hundreds nanometers. Importantly, can be used as templates assembly functional components into three-dimensional high precision controlled stoichiometry. This is often beyond reach other nanofabrication techniques. In this Perspective, we highlight capability realization novel nanophotonic systems. First, introduce basic principles designing fabrication structures. Subsequently, review recent advances applications nanoplasmonics, single-molecule super-resolution fluorescent imaging, well hybrid photonic We conclude by outlining future prospects advanced systems tailored functionalities.

Language: Английский

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DNA Nanostructures at the Interface with Biology

Chem, Journal Year: 2018, Volume and Issue: 4(3), P. 495 - 521

Published: March 1, 2018

Language: Английский

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