Rapid MALDI mass spectrometry imaging for surgical pathology DOI Creative Commons
Sankha S. Basu, Michael S. Regan,

Elizabeth C. Randall

et al.

npj Precision Oncology, Journal Year: 2019, Volume and Issue: 3(1)

Published: July 4, 2019

Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid method, completed under 5 including sample preparation analysis, providing workflow compatible with the frozen section procedure.

Language: Английский

Tracing oncogene-driven remodelling of the intestinal stem cell niche DOI
Min Kyu Yum, Seungmin Han,

Juergen Fink

et al.

Nature, Journal Year: 2021, Volume and Issue: 594(7863), P. 442 - 447

Published: June 2, 2021

Language: Английский

Citations

96
HBV-Integration Studies in the Clinic: Role in the Natural History of Infection DOI Creative Commons
Teresa Pollicino,

Giuseppe Caminiti

Viruses, Journal Year: 2021, Volume and Issue: 13(3), P. 368 - 368

Published: Feb. 26, 2021

Hepatitis B virus (HBV) infection is a major global health problem causing acute and chronic liver disease that can lead to cirrhosis hepatocellular carcinoma (HCC). HBV covalently closed circular DNA (cccDNA) essential for viral replication the establishment of persistent infection. Integrated represents another stable form regularly observed in livers infected patients. integration into host genome occurs early after It common occurrence during life cycle, it has been detected all phases long considered be main contributor tumorigenesis. The recent development highly sensitive detection methods research models led clarification some molecular pathogenic aspects integration. Though does not replication-competent transcripts, act as source RNA proteins, which may contribute determining HBV-specific T-cell exhaustion favoring persistence. relationship between immune response microenvironment might closely related progression HBV-related diseases. While many new antiviral agents aimed at cccDNA elimination or silencing have developed, integrated remains difficult therapeutic challenge.

Language: Английский

Citations

95
Matrix stiffness mechanically conditions EMT and migratory behavior of oral squamous cell carcinoma DOI Creative Commons
Bibiana Franzen Matte, Aditya Kumar, Jesse K. Placone

et al.

Journal of Cell Science, Journal Year: 2018, Volume and Issue: unknown

Published: Jan. 1, 2018

Tumors are composed of heterogeneous phenotypes, each having different sensitivities to the microenvironment. One microenvironment characteristic–matrix stiffness–helps regulate malignant transformation and invasion in mammary tumors, but its influence on oral squamous cell carcinoma (OSCC) is unclear. We observed that stiff matrices, a highly invasive OSCC line with low E-cad/N-cad ratio (InvH/E:NL; SCC25) had increased migration velocity decreased adhesion strength compared poorly high (InvL/E:NH; Cal27). However, InvL/E:NH cells acquire mesenchymal signature begin migrate faster when exposed prolonged time niche, suggesting could be mechanically conditioned. migrated due focal assembly, which reduced increasing integrin affinity divalent cation concentrations. Mirroring these data human patients, we collagen organization, an indicator matrix stiffness, was advanced disease correlated early recurrence. Consistent epithelial our suggests sensitive their contribution tumor progression mediated part by this sensitivity.

Language: Английский

Citations

90
PPAR-γ Modulators as Current and Potential Cancer Treatments DOI Creative Commons

Tiange Chi,

Mina Wang, Xu Wang

et al.

Frontiers in Oncology, Journal Year: 2021, Volume and Issue: 11

Published: Sept. 23, 2021

Worldwide, cancer has become one of the leading causes mortality. Peroxisome Proliferator-Activated Receptors (PPARs) is a family critical sensors lipids as well regulators diverse metabolic pathways. They are also equipped with capability to promote eNOS activation, regulate immunity and inflammation response. Aside from established properties, emerging discoveries made in PPAR's functions field. All considerations given, there exists great potential PPAR modulators which may hold management cancers. In particular, PPAR-γ, most expressed subtype adipose tissues two isoforms different tissue distribution, been proven be able inhibit cell proliferation, induce cycle termination apoptosis multiple cells, intercellular adhesion, cripple inflamed state tumor microenvironment, both on transcriptional protein level. However, despite multi-functionalities, safety PPAR-γ still clinical concern terms dosage, drug interactions, types stages, etc. This review aims consolidate current applications modulators, challenges applying treatment, laboratory settings. We sincerely hope provide comprehensive perspective prospect applicability field treatment.

Language: Английский

Citations

85
Rapid MALDI mass spectrometry imaging for surgical pathology DOI Creative Commons
Sankha S. Basu, Michael S. Regan,

Elizabeth C. Randall

et al.

npj Precision Oncology, Journal Year: 2019, Volume and Issue: 3(1)

Published: July 4, 2019

Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid method, completed under 5 including sample preparation analysis, providing workflow compatible with the frozen section procedure.

Language: Английский

Citations

82