Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver

Nature Cell Biology, Journal Year: 2015, Volume and Issue: 17(6), P. 816 - 826

Published: May 18, 2015

Language: Английский

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Reactive Oxygen Species (ROS)-Based Nanomedicine

Chemical Reviews, Journal Year: 2019, Volume and Issue: 119(8), P. 4881 - 4985

Published: April 11, 2019

Reactive oxygen species (ROS) play an essential role in regulating various physiological functions of living organisms. The intrinsic biochemical properties ROS, which underlie the mechanisms necessary for growth, fitness, or aging organisms, have been driving researchers to take full advantage these active chemical contributing medical advances. Thanks remarkable advances nanotechnology, great varieties nanomaterials with unique ROS-regulating explored guide temporospatial dynamic behaviors ROS biological milieu, contributes emergence a new-generation therapeutic methodology, i.e., nanomaterial-guided vivo evolution therapy. interdependent relationship between and their corresponding chemistry, biology, nanotherapy leads us propose concept "ROS science", is believed be emerging scientific discipline that studies mechanisms, effects, nanotherapeutic applications ROS. In this review, state-of-art concerning recent progresses on ROS-based nanotherapies summarized detail, emphasis underlying material chemistry by are generated scavenged improved outcomes. Furthermore, key issues cross-disciplinary fields also discussed, aiming unlock innate powers optimized efficacies. We expect our demonstration evolving field will beneficial further development fundamental researches clinical applications.

Language: Английский

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Regulation of immune responses by extracellular vesicles

Nature reviews. Immunology, Journal Year: 2014, Volume and Issue: 14(3), P. 195 - 208

Published: Feb. 25, 2014

Language: Английский

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Optimized exosome isolation protocol for cell culture supernatant and human plasma

Journal of Extracellular Vesicles, Journal Year: 2015, Volume and Issue: 4(1)

Published: Jan. 1, 2015

Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis disease. However, there is currently limited information elucidating most efficient methods for obtaining high yields pure exosomes, subset extracellular vesicles, from cell culture supernatant complex biological fluids such as plasma. To this end, we comprehensively characterize variety exosome isolation protocols their efficiency, yield purity isolated exosomes. Repeated ultracentrifugation steps can reduce quality preparations leading to lower yield. We show that concentration conditioned media using ultrafiltration devices results increased vesicle when compared traditional protocols. our data on Non-Small-Cell Lung Cancer (NSCLC) SK-MES-1 line demonstrates choice concentrating device greatly impact find centrifuge-based are more appropriate than pressure-driven allow rapid exosomes both NSCLC fluids. In fact date, no protocol detailing utilizing current commercial cells patient samples has been described. Utilizing tunable resistive pulse sensing protein analysis, provide comparative analysis 4 techniques, indicating efficacy preparation purity. Our demonstrate precipitation plasma least whereas size exclusion comparable density gradient purification have identified shortcomings common potential standardized method effective, reproducible be utilized various starting materials. believe will extensive application growing field research.

Language: Английский

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Depletion of microglia and inhibition of exosome synthesis halt tau propagation

Nature Neuroscience, Journal Year: 2015, Volume and Issue: 18(11), P. 1584 - 1593

Published: Oct. 5, 2015

Language: Английский

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Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

Nature Cell Biology, Journal Year: 2018, Volume and Issue: 20(3), P. 332 - 343

Published: Feb. 14, 2018

Language: Английский

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Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting

Journal of Extracellular Vesicles, Journal Year: 2015, Volume and Issue: 4(1)

Published: Jan. 1, 2015

Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication in a diverse range biological processes. For future therapeutic applications and for EV biology research general, understanding the vivo fate EVs is utmost importance. Here we studied biodistribution mice after systemic delivery. were isolated from 3 different mouse cell sources, including dendritic cells (DCs) derived bone marrow, labelled with near-infrared lipophilic dye. Xenotransplantation was further carried out cross-species comparison. The reliability labelling technique confirmed by sucrose gradient fractionation, organ perfusion supported immunohistochemical staining using CD63-EGFP probed vesicles. While accumulated mainly liver, spleen, gastrointestinal tract lungs, differences related to origin detected. tumour tissue tumour-bearing and, introduction rabies virus glycoprotein-targeting moiety, they found more readily acetylcholine-receptor-rich organs. In addition, route administration dose injected influenced pattern. This first extensive investigation comparing impact several variables, results which implications design feasibility studies EVs.

Language: Английский

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New Technologies for Analysis of Extracellular Vesicles

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(4), P. 1917 - 1950

Published: Jan. 31, 2018

Extracellular vesicles (EVs) are diverse, nanoscale membrane actively released by cells. Similar-sized can be further classified (e.g., exosomes, microvesicles) based on their biogenesis, size, and biophysical properties. Although initially thought to cellular debris, thus under-appreciated, EVs now increasingly recognized as important vehicles of intercellular communication circulating biomarkers for disease diagnoses prognosis. Despite clinical potential, the lack sensitive preparatory analytical technologies poses a barrier translation. New platforms including molecular ones being developed address these challenges. Recent advances in field expected have far-reaching impact both basic translational studies. This article aims present comprehensive critical overview emerging EV detection applications.

Language: Английский

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Extracellular vesicles as a next-generation drug delivery platform

Nature Nanotechnology, Journal Year: 2021, Volume and Issue: 16(7), P. 748 - 759

Published: July 1, 2021

Language: Английский

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Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper

Journal of Extracellular Vesicles, Journal Year: 2015, Volume and Issue: 4(1)

Published: Jan. 1, 2015

Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c) immune‐modulatory regenerative therapies (d) drug delivery. The translation into clinical requires the categorization EV‐based therapeutics compliance with existing regulatory frameworks. classification defines subsequent requirements manufacturing, quality control investigation, it is major importance define whether considered active components or primarily delivery vehicles. For an effective particularly safe practice, high level cooperation between researchers, clinicians competent authorities essential. In this position statement, basic scientists, members International Society Vesicles (ISEV) European Cooperation Science Technology (COST) program Union, namely Network on Microvesicles Exosomes Health Disease (ME‐HaD), summarize recent developments current knowledge therapies. Aspects safety must be pharmaceutical manufacturing application highlighted. Production processes discussed. Strategies promote future studies addressed.

Language: Английский

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