Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Journal of Extracellular Vesicles, Journal Year: 2018, Volume and Issue: 7(1)

Published: Nov. 23, 2018

ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively pure preparations, characterize properly. International Society for Extracellular Vesicles (ISEV) proposed Minimal Information Studies (“MISEV”) guidelines field 2014. We now update “MISEV2014” based on evolution knowledge four years. An important point consider is that ascribing function EVs general, or EVs, requires reporting information beyond mere description crude, potentially contaminated, heterogeneous preparation. For example, claims exosomes are endowed exquisite activities remain support experimentally, given our still limited their molecular machineries biogenesis release, compared biophysically similar EVs. MISEV2018 include tables outlines suggested protocols steps follow document EV‐associated functional activities. Finally, checklist provided summaries key points.

Language: Английский

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Progress in Exosome Isolation Techniques

Theranostics, Journal Year: 2017, Volume and Issue: 7(3), P. 789 - 804

Published: Jan. 1, 2017

Exosomes are one type of membrane vesicles secreted into extracellular space by most types cells. In addition to performing many biological functions particularly in cell-cell communication, cumulative evidence has suggested that several entities exosomes like proteins and microRNAs closely associated with the pathogenesis human malignancies they may serve as invaluable biomarkers for disease diagnosis, prognosis, therapy. This provides a commanding impetus growing demands simple, efficient, affordable techniques isolate exosomes. Capitalizing on physicochemical biochemical properties exosomes, number have been developed isolation article summarizes advances exosome an emphasis their mechanism, performance, challenges, prospects. We hope this will provide overview techniques, opening up new perspectives towards development more innovative strategies devices time saving, cost effective, efficient isolations from wide range matrices.

Language: Английский

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Integrating liquid biopsies into the management of cancer

Nature Reviews Clinical Oncology, Journal Year: 2017, Volume and Issue: 14(9), P. 531 - 548

Published: March 2, 2017

Language: Английский

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New Technologies for Analysis of Extracellular Vesicles

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(4), P. 1917 - 1950

Published: Jan. 31, 2018

Extracellular vesicles (EVs) are diverse, nanoscale membrane actively released by cells. Similar-sized can be further classified (e.g., exosomes, microvesicles) based on their biogenesis, size, and biophysical properties. Although initially thought to cellular debris, thus under-appreciated, EVs now increasingly recognized as important vehicles of intercellular communication circulating biomarkers for disease diagnoses prognosis. Despite clinical potential, the lack sensitive preparatory analytical technologies poses a barrier translation. New platforms including molecular ones being developed address these challenges. Recent advances in field expected have far-reaching impact both basic translational studies. This article aims present comprehensive critical overview emerging EV detection applications.

Language: Английский

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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(2)

Published: Feb. 1, 2024

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.

Language: Английский

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Isolation of Extracellular Vesicles: General Methodologies and Latest Trends

BioMed Research International, Journal Year: 2018, Volume and Issue: 2018, P. 1 - 27

Published: Jan. 1, 2018

Background . Extracellular vesicles (EVs) play an essential role in the communication between cells and transport of diagnostically significant molecules. A wide diversity approaches utilizing different biochemical properties EVs a lack accepted protocols make data interpretation very challenging. Scope Review This review consolidates on classical state-of-the-art methods for isolation EVs, including exosomes, highlighting advantages disadvantages each method. Various characteristics individual methods, efficiency, EV yield, isolated labor consumption are compared. Major Conclusions mixed population is obtained most studies all used methods. The analyzed sample should be taken into account when planning experiment aimed at studying using these vesicles. problem adequate still remains; it might not possible to develop universal method but available can towards solving particular types problems. General Significance With use diagnosis therapy various diseases evaluation existing one key problems modern biology medicine.

Language: Английский

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Methodological Guidelines to Study Extracellular Vesicles

Circulation Research, Journal Year: 2017, Volume and Issue: 120(10), P. 1632 - 1648

Published: May 11, 2017

Owing to the relationship between extracellular vesicles (EVs) and physiological pathological conditions, interest in EVs is exponentially growing. hold high hopes for novel diagnostic translational discoveries. This review provides an expert-based update of recent advances methods study summarizes currently accepted considerations recommendations from sample collection isolation, detection, characterization EVs. Common misconceptions methodological pitfalls are highlighted. Although found all body fluids, this review, we will focus on human blood, not only our most complex but also interesting fluid cardiovascular research.

Language: Английский

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Extracellular vesicle isolation and characterization: toward clinical application

Journal of Clinical Investigation, Journal Year: 2016, Volume and Issue: 126(4), P. 1152 - 1162

Published: March 31, 2016

Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well protein RNA profiles, have been described. EVs are known to play key roles cell-cell communication, acting proximally systemically. This Review discusses the nature EV subtypes, strategies for isolating from both cell-culture media body fluids, procedures quantifying EVs. We also discuss proteins selectively enriched sMVs that potential use markers discriminate between various applications clinical diagnosis.

Language: Английский

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Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics

Theranostics, Journal Year: 2020, Volume and Issue: 10(8), P. 3684 - 3707

Published: Jan. 1, 2020

Exosomes are small extracellular vesicles with diameters of 30-150 nm.In both physiological and pathological conditions, nearly all types cells can release exosomes, which play important roles in cell communication epigenetic regulation by transporting crucial protein genetic materials such as miRNA, mRNA, DNA.Consequently, exosome-based disease diagnosis therapeutic methods have been intensively investigated.However, any natural science field, the in-depth investigation exosomes relies heavily on technological advances.Historically, two main technical hindrances that restricted basic applied researches include, first, how to simplify extraction improve yield and, second, effectively distinguish from other vesicles, especially functional microvesicles.Over past few decades, although a standardized exosome isolation method has still not become available, number techniques established through exploration biochemical physicochemical features exosomes.In this work, comprehensively analyzing progresses separation strategies, we provide panoramic view current techniques, providing perspectives toward development novel approaches for high-efficient various biological matrices.In addition, perspective therapeutics, emphasize issue quantitative microvesicle separation.

Language: Английский

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A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Jan. 23, 2020

Extracellular vesicles (EVs) are nano-sized containing nucleic acid and protein cargo that released from a multitude of cell types have gained significant interest as potential diagnostic biomarkers. Human serum is rich source readily accessible EVs; however, the separation EVs proteins non-EV lipid particles represents considerable challenge. In this study, we compared most commonly used isolation techniques, either alone or in combination, for 200 µl human their present serum. The size yield isolated by each method was determined nanoparticle tracking analysis, with variation particle distribution being to determine relative impact lipoproteins aggregates on EV population. Purification soluble calculating ratio count concentration. Finally, lipoprotein co-isolated Western blot analysis markers APOB APOE. Overall, study reveals choice procedure significantly impacts serum, together presence contaminants.

Language: Английский

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