Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)
Published: July 1, 2024
The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.
Language: Английский
Citations
94
Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2024, Volume and Issue: unknown, P. 232 - 271
Published: Jan. 1, 2024
Language: Английский
Citations
5
Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12
Published: Jan. 28, 2025
The evolution of stem cell-based heart models from cells and tissues to organoids assembloids recently synthetic embryology gastruloids, is poised revolutionize our understanding cardiac development, congenital adult diseases, patient customized therapies. Human induced pluripotent cell-derived cardiomyocytes (hiPSC-CMs) have already been integrated into transplantable patches are in preclinical efforts reverse fibrotic scarring myocardial infarctions. To inform on the complexity multi-tissue morphogenic needed that replicate fundamental components function organogenesis vitro which require a deep development. Organoid assembloid capture selected multicellular processes, such as chamber formation priming events for vascularization. Gastruloid offer deeper insights mimic multi-staged developmental vivo including established fields, crescent tube development along with vascular systemic foundation even further steps. human Elongating Multi-Lineage Organized Cardiac (EMLOC) gastruloid model captures these stages additional genesis, patterned vascularization, extrinsic central intrinsic nervous system (CNS-ICNS) integration guided by spatiotemporal processes neural crest cells. new previously hidden provide powerful platforms addressing disease extends beyond cardiomyocytes, arrhythmogenic defects, injury interactions, spinal cord injuries. holistic view emerging will reveal unprecedented detail drive transformative state-of-the-art innovative applications health.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3085 - 3085
Published: March 27, 2025
In recent years, the management of bone defects in regenerative medicine and orthopedic surgery has been subject extensive research efforts. The complexity fractures loss arising from trauma, degenerative conditions, or congenital disorders necessitates innovative therapeutic strategies to promote effective healing. Although tissue exhibits an intrinsic capacity, critical-sized can severely compromise this process, often requiring grafts substitutes. Tissue engineering approaches within have introduced novel possibilities for addressing nonunions challenging refractory conventional treatment methods. Key components field include stem cells, bioactive growth factors, biocompatible scaffolds, with a strong focus on advancements substitute materials. Both natural synthetic substitutes present distinct characteristics applications. Natural grafts-comprising autologous, allogeneic, xenogeneic materials-offer biological advantages, while alternatives, including biodegradable non-biodegradable biomaterials, provide structural versatility reduced immunogenicity. This review provides comprehensive analysis diverse grafting alternatives utilized surgery, emphasizing persistent challenges. By exploring both substitutes, work offers in-depth examination cutting-edge solutions, fostering further innovation complex defects.
Language: Английский
Citations
0
Cell Discovery, Journal Year: 2025, Volume and Issue: 11(1)
Published: April 21, 2025
Abstract The process of a single-celled zygote developing into complex multicellular organism is precisely regulated at spatial and temporal levels in vivo. However, understanding the mechanisms underlying development, particularly humans, has been constrained by technical ethical limitations associated with studying natural embryos. Harnessing intrinsic ability embryonic stem cells (ESCs) to self-organize when induced assembled, researchers have established several embryo models as alternative approaches early development vitro. Recent studies revealed critical role extraembryonic development; many groups created more sophisticated precise ESC-derived incorporating cell lines, such trophoblast (TSCs), mesoderm (EXMCs), endoderm (XENs, rodents), hypoblast (in primates). Here, we summarize characteristics existing mouse human review recent advancements models.
Language: Английский
Citations
0
Stem Cell Reviews and Reports, Journal Year: 2025, Volume and Issue: unknown
Published: May 20, 2025
Abstract Independent traditional gametes and recent advances in stem cell biology have made it possible to create synthetic embryo models (SEMs), altering our capacity study early human development, congenital diseases, regenerative medicine. By recreating key developmental events vitro, these provide unmatched insights into embryogenesis creative platforms for disease modeling, drug discovery, individualized therapy. The quick development SEM research raises serious ethical, legal, regulatory questions that call creating transparent control systems. methods applied fabrication, their biomedical applications, the moral issues connected with use are investigated this review. We also look at future directions, including enhancing ethical frameworks, adding artificial intelligence, increasing model fidelity, encouraging public participation. Through multidisciplinary cooperation, SEMs might address problems transform biology, advancing scientific advancement.
Language: Английский
Citations
0
Zeitschrift für Naturforschung C, Journal Year: 2025, Volume and Issue: unknown
Published: May 22, 2025
Abstract Stem cell therapy has emerged as a groundbreaking approach in regenerative medicine, offering immense potential for tissue regeneration and wound healing. cells, with their ability to self-renew differentiate into specialized types, provide innovative therapeutic strategies variety of medical conditions. Key stem including embryonic, induced pluripotent, adult cells such mesenchymal hematopoietic play pivotal roles processes repair. In regeneration, replenish damaged or necrotic by differentiating types like bone, muscle, nerve thus restoring the structural functional integrity tissues. healing, stimulate angiogenesis, generate new skin modulate immune responses enhance This multifaceted paved way clinical applications cardiovascular, neurological, musculoskeletal, autoimmune disorders, well burn injuries. review highlights recent advancements therapy, exploring its addressing challenges rejection, ethical concerns, scalability, need long-term trials. The article underscores importance continued research fully realize transformative modern medicine.
Language: Английский
Citations
0
Theriogenology, Journal Year: 2024, Volume and Issue: 232, P. 109 - 116
Published: Nov. 6, 2024
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: May 1, 2024
ABSTRACT Over the last decade, single-cell approaches have become gold standard for studying gene expression dynamics, cell heterogeneity, and states within samples. Before advances, feasibility of capturing dynamic cellular landscape rapid transitions during early development was limited. In this paper, we designed a robust pipeline to perform nuclei analysis on mouse embryos from E6.5 E8, corresponding onset completion gastrulation. Gastrulation is fundamental process that establishes three germinal layers: mesoderm, ectoderm, endoderm, which are essential organogenesis. Extensive literature available omics applied WT perigastrulating embryos. However, mutant still scarce often limited FACS-sorted populations. This partially due technical constraints associated with need genotyping, timed pregnancies, count desired genotypes per pregnancy, number cells embryo at these stages. Here, present methodology overcome limitations. method breeding pregnancy guidelines achieve higher chance synchronized pregnancies genotypes. Optimization steps in isolation coupled FAST genotyping protocol (3 hours) allow microdroplet-based be performed same day, ensuring high viability results. We also include optimal isolations Thus, increase gastrulation stage. anticipate will facilitate how mutations shape gastrula. SUMMARY establish high-quality suspensions gastrulating sequencing single nuclei.
Language: Английский
Citations
1
Journal of Veterinary Science, Journal Year: 2024, Volume and Issue: 25(3)
Published: Jan. 1, 2024
The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages embryonic development reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic highly complex
Language: Английский
Citations
1