Effects of meglumine antimoniate and allopurinol treatment on the fecal microbiome profile in dogs with leishmaniosis DOI Creative Commons
Joan Martí‐Carreras,

M S Carrasco,

Marc Noguera-Julián

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Abstract The combination of meglumine antimoniate and allopurinol is considered one the most effective treatments for canine leishmaniosis caused by Leishmania infantum . This study investigated effects this treatment on gut microbiome 10 dogs from Spain, Portugal, Italy via fecal shotgun metagenomic sequencing over six months. Dogs were sampled at baseline (BL) (M1) (M6) months post-treatment. -infected dominated Prevotella , Collinsella Bacteroides Blautia with individual variability being primary determinant composition. No significant changes in alpha diversity (Shannon index, gene number) or beta (Bray-Curtis dissimilarity, UniFrac distance) detected between pre- post-treatment time points, suggesting that does not disrupt microbiota. Minor trends taxonomic shifts noted, slight increases Bifidobacterium pseudocantenulatum tanakaei Slackia piriformis after treatment, but these statistically correction multiple testing. Linear discriminant analysis multivariable modeling confirmed microbial community structure was resilient to effects. Individual-specific differences accounted 52% observed variability, underscoring personalized nature microbiota dogs. Importantly, no adverse disruptions detected, even prolonged use. highlights robustness during antileishmanial therapy use without compromising health. Further studies larger cohorts are recommended confirm findings explore functional roles modulating immune responses

Language: Английский

Early life gut microbiome and its impact on childhood health and chronic conditions DOI Creative Commons

Harold Núñez,

Pamela A. Nieto,

Ruben A. T. Mars

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 7, 2025

The development of the gut microbiome is crucial to human health, particularly during first three years life. Given its role in immune development, disturbances establishment process may have long term consequences. This review summarizes evidence for these claims, highlighting compositional changes this critical period life as well factors that affect development. Based on and animal data, we conclude early-life a determinant long-term impacting physiological, metabolic, processes. field faces challenges. Some challenges are technical, such lack standardized stool collection protocols, inconsistent DNA extraction methods, outdated sequencing technologies. Other methodological: small sample sizes, longitudinal studies, poor control confounding variables. To address limitations, advocate more robust research methodologies better understand microbiome's health disease. Improved methods will lead reliable studies deeper understanding impact outcomes.

Language: Английский

Citations

4
The Effect of Oral Microbiota on the Severity and Prognosis of Stroke DOI Open Access
F. A. Yusupov, A. A. Yuldashev,

A.A. Yusupov

et al.

Bulletin of Science and Practice, Journal Year: 2025, Volume and Issue: 11(1), P. 124 - 145

Published: Jan. 15, 2025

The microbiome of the oral cavity plays an important role in development and progression various somatic diseases, including stroke. Studies show that bacteria living can enter bloodstream contribute to inflammatory processes, which increases risk cardiovascular disease It was found 79% studied stroke-related blood clots, DNA commonly cavity, such as streptococci Viridans species, found. Periodontal pathogenic microorganisms cause chronic inflammation atherosclerosis, is one main factors for Dysbiosis also associated with increase level proinflammatory cytokines, worsen condition vessels likelihood thrombosis. Thus, maintaining health timely treatment dental diseases have a significant impact on reducing Understanding relationship between state system opens up new horizons preventive measures therapeutic approaches patients at high

Language: Английский

Citations

1
Refining microbiome diversity analysis by concatenating and integrating dual 16S rRNA amplicon reads DOI Creative Commons
Kyoung Su Kim, Jihye Noh, Bong Soo Kim

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 12, 2025

Understanding the role of human gut microbiota in health and disease requires insights into its taxonomic composition functional capabilities. This study evaluates whether concatenating paired-end reads enhances data output for microbiome analysis compared to merging approach across various regions 16S rRNA gene. We assessed this both mock communities Korean cohorts with or without ulcerative colitis. Our results indicate that using direct joining method V1-V3 V6-V8 improves resolution (ME) post-sequencing data. While predicting microbial function based on sequencing has inherent limitations, integrating from enhanced predictions. was confirmed by whole metagenome (WMS) cohorts, where our improved taxa detection lost ME method. Thus, we propose integrated dual technique serves as a valuable tool research bridging gap between amplicon WMS.

Language: Английский

Citations

1
Modeling Microbial Communities: Perspective and Challenges DOI Creative Commons
Lavanya Raajaraam, Karthik Raman

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 2260 - 2270

Published: Aug. 16, 2024

Microbial communities are immensely important due to their widespread presence and profound impact on various facets of life. Understanding these complex systems necessitates mathematical modeling, a powerful tool for simulating predicting microbial community behavior. This review offers critical analysis metabolic modeling highlights key areas that would greatly benefit from broader discussion collaboration. Moreover, we explore the challenges opportunities linked intricate nature communities, spanning data generation, validation. We confident ongoing advancements in techniques, such as machine learning, coupled with interdisciplinary collaborations, will unlock full potential across diverse applications.

Language: Английский

Citations

7
Oral Microbiome Dysbiosis as a Risk Factor for Stroke: A Comprehensive Review DOI Creative Commons
Georgy Leonov, Diana Salikhova, Antonina V. Starodubova

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1732 - 1732

Published: Aug. 22, 2024

Stroke represents a significant global health burden, with substantial impact on mortality, morbidity, and long-term disability. The examination of stroke biomarkers, particularly the oral microbiome, offers promising avenue for advancing our understanding factors that contribute to risk developing strategies mitigate risk. This review highlights correlations between diseases, such as periodontitis caries, onset stroke. Periodontal pathogens within microbiome have been identified contributing factor in exacerbation stroke, including obesity, dyslipidemia, atherosclerosis, hypertension, endothelial dysfunction. alteration may these conditions, emphasizing vital role prevention cardiovascular disease. integration dental medical practices enhancing efforts improving patient outcomes.

Language: Английский

Citations

5
Metagenomic approaches and opportunities in arid soil research DOI
Muhammad Ejaz, Kareem Rady Badr, Zahoor Ul Hassan

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 953, P. 176173 - 176173

Published: Sept. 10, 2024

Language: Английский

Citations

5
Intestinal Insights: The Gut Microbiome’s Role in Atherosclerotic Disease: A Narrative Review DOI Creative Commons

Luana Alexandrescu,

Adrian Paul Suceveanu,

Alina Mihaela Stăniguţ

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2341 - 2341

Published: Nov. 16, 2024

Recent advances have highlighted the gut microbiota as a significant contributor to development and progression of atherosclerosis, which is an inflammatory cardiovascular disease (CVD) characterized by plaque buildup within arterial walls. The microbiota, consisting diverse collection microorganisms, impacts host's metabolism, immune responses, lipid processing, all contribute atherosclerosis. This review explores complex mechanisms through dysbiosis promotes atherogenesis. We emphasize potential integrating modulation with traditional care, offering holistic approach managing Important pathways involve translocation microbial components, metabolism metabolites such trimethylamine-N-oxide (TMAO), production short-chain fatty acids (SCFAs) that influence vascular health. Studies reveal distinct profiles in atherosclerosis patients, increased pathogenic bacteria (

Language: Английский

Citations

4
Unveiling the Resistome Landscape in Peri-Implant Health and Disease DOI Open Access
Lucinda J. Bessa, Conceição Egas, João Botelho

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(3), P. 931 - 931

Published: Jan. 31, 2025

Background: The human oral microbiome is a critical reservoir for antibiotic resistance; however, subgingival peri-implant biofilms remain underexplored in this context. We aimed to explore the prevalence and distribution of resistance genes (ARGs) metagenomes derived from saliva biofilms. Methods: A total 100 metagenome datasets 40 individuals were retrieved Sequence Read Archive (SRA) database. Of these, 20 had exclusively healthy implants both affected with peri-implantitis. ARGs their taxonomic assignments identified using ABRicate tool, plasmid detection was performed PlasmidFinder. Results: Four replicons 72 metagenomes, 55 distinct 13 classes detected 89 metagenomes. conferring macrolides-lincosamides-streptogramins, tetracyclines, beta-lactams, fluoroquinolones most prevalent. msr(D) mef(A) showed highest prevalence, except samples implants, where ranked fourth. pairwise PERMANOVA principal coordinate analysis based on Jaccard distances revealed that exhibited significantly greater ARG diversity than biofilm (p < 0.05). However, no significant differences observed between peri-implantitis-affected groups > origins also analyzed understand potential impact microbial communities. Conclusions: Resistome profiles associated health disease higher salivary abundance compared samples.

Language: Английский

Citations

0
Unveiling the microbial influence: bacteria’s dual role in tumor metastasis DOI Creative Commons

Li-Ying Lin,

Dongyan Zhang

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 14, 2025

As cancer research advances, the intricate relationship between microbiome and is gaining heightened recognition, especially concerning tumor metastasis, where bacterial involvement becomes increasingly complex. This review seeks to systematically examine dual roles of bacteria in metastasis process, encompassing both mechanisms that facilitate inhibitory effects exerted by specific microorganisms. We explore through which influence cell migration inducing chronic inflammation, evading host immune responses, remodeling ECM. Moreover, immunomodulatory potential probiotics genetically engineered offers promising prospects for prevention treatment metastasis. article elucidates complexity emerging frontiers examining clinical significance as biomarkers evaluating antibiotic usage on metastatic process. posit comprehending biological characteristics bacteria, a critical component microenvironment, will offer innovative strategies theoretical foundations treatment. Furthermore, this explores future directions, including application technologies bacteria-based therapeutic strategies, thereby offering valuable perspective development novel anti-cancer approaches.

Language: Английский

Citations

0
Operationalizing Team Science at the Academic Cancer Center Network to Unveil the Structure and Function of the Gut Microbiome DOI Open Access
Kevin McDonnell

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(6), P. 2040 - 2040

Published: March 17, 2025

Oncologists increasingly recognize the microbiome as an important facilitator of health well a contributor to disease, including, specifically, cancer. Our knowledge etiologies, mechanisms, and modulation states that ameliorate or promote cancer continues evolve. The progressive refinement adoption “omic” technologies (genomics, transcriptomics, proteomics, metabolomics) utilization advanced computational methods accelerate this evolution. academic center network, with its immediate access extensive, multidisciplinary expertise scientific resources, has potential catalyze research. Here, we review our current understanding role gut in prevention, predisposition, response therapy. We underscore promise operationalizing network uncover structure function microbiome; highlight unique microbiome-related expert resources available at City Hope Comprehensive Cancer Center example team science achieve novel clinical discovery.

Language: Английский

Citations

0