Tonotopic organization of auditory cortex in awake marmosets revealed by multi-modal wide-field optical imaging

Current Research in Neurobiology, Journal Year: 2024, Volume and Issue: 6, P. 100132 - 100132

Published: Jan. 1, 2024

Tonotopic organization of the auditory cortex has been extensively studied in many mammalian species using various methodologies and physiological preparations. Tonotopy mapping primates, however, is more limited due to constraints such as cortical folding, use anesthetized subjects, methodology. Here we applied a combination through-skull through-window intrinsic optical signal imaging, wide-field calcium neural probe recording techniques awake marmosets (Callithrix jacchus), New World monkey with most its located on flat brain surface. Coarse tonotopic gradients, including recently described rostral-temporal (RT) parabelt gradient, were revealed by imaging signals subsequently validated single-unit recording. Furthermore, these gradients observed detail through chronically implanted cranial windows additional verifications experimental design. Moreover, tonotopy mapped intrinsic-signal methods was verified an AAV-GCaMP labeled subject. After validations further effort expand field view rostrally both windowed putative gradient area RT, which not previously standard model primate cortex. Together, results provide comprehensive data unprecedented coverage details rostral proportion support caudal-rostrally arranged mesoscale at least three repeats functional cortex, similar ventral stream visual

Language: Английский

---

Mesoscopic landscape of cortical functions revealed by through-skull wide-field optical imaging in marmoset monkeys

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: April 26, 2022

Abstract The primate cerebral cortex is organized into specialized areas representing different modalities and functions along a continuous surface. functional maps across the cortex, however, are often investigated single modality at time (e.g., audition or vision). To advance our understanding of complex landscape cortical functions, here we develop polarization-gated wide-field optical imaging method for measuring through un-thinned intact skull in awake marmoset monkeys ( Callithrix jacchus ), species featuring smooth cortex. Using this method, adjacent auditory, visual, somatosensory cortices noninvasively parcellated individual subjects with detailed tonotopy, retinotopy, somatotopy. An additional pure-tone-responsive tonotopic gradient discovered auditory face-patch sensitive to motion lower-center visual field localized near an region frequencies conspecific vocalizations. This through-skull landscape-mapping approach provides new opportunities how coordinated enable real-world behaviors.

Language: Английский

---

General Post‐Regulation Strategy of AIEgens’ Photophysical Properties for Intravital Two‐Photon Fluorescence Imaging

Advanced Science, Journal Year: 2024, Volume and Issue: 11(38)

Published: Aug. 9, 2024

Fluorogens with aggregation-induced emission (AIEgens) are promising agents for two-photon fluorescence (TPF) imaging. However, AIEgens' photophysical properties fixed and unoptimizable once synthesized. Therefore, it is urgent meaningful to explore an efficient post-regulation strategy optimize properties. Herein, a general reported. By simply tuning the ratio of inert AIEgens within binary nanoparticles (BNPs), quantum yield absorption cross-section functional enhanced by 8.7 5.4 times respectively, which not achievable conventional strategies, notorious phototoxicity almost eliminated. The experimental results, theoretical simulation, mechanism analysis demonstrated its feasibility generality. BNPs enabled deep cerebrovascular network imaging ≈1.10 mm depth metastatic cancer cell detection single-cell resolution. Furthermore, TPF quality improved self-supervised denoising algorithm. proposed molecular opened new avenue efficiently boost consequently quality.

Language: Английский

---

Deep Learning Enhanced Label-Free Action Potential Detection Using Plasmonic-Based Electrochemical Impedance Microscopy

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11299 - 11308

Published: July 2, 2024

Measuring neuronal electrical activity, such as action potential propagation in cells, requires the sensitive detection of weak signal with high spatial and temporal resolution. None existing tools can fulfill this need. Recently, plasmonic-based electrochemical impedance microscopy (P-EIM) was demonstrated for label-free mapping ignition potentials neuron cells subcellular However, limited by signal-to-noise ratio high-speed P-EIM video, achieved averaging 90 cycles signals. Such extensive is not desired may always be feasible due to factors desensitization. In study, we utilized advanced processing techniques detect extracted signals fewer averaged cycles. Matched filtering successfully detected few five Long short-term memory (LSTM) recurrent neural network best performance able single-cycle stimulated [satisfactory area under receiver operating characteristic curve (AUC) equal 0.855]. Therefore, show that deep learning-based dramatically improve usability

Language: Английский

---

An anatomical and connectivity atlas of the marmoset cerebellum

Cell Reports, Journal Year: 2023, Volume and Issue: 42(5), P. 112480 - 112480

Published: May 1, 2023

The cerebellum is essential for motor control and cognitive functioning, engaging in bidirectional communication with the cerebral cortex. common marmoset, a small non-human primate, offers unique advantages studying cerebello-cerebral circuits. However, marmoset not well described published resources. In this study, we present comprehensive atlas of comprising (1) fine-detailed anatomical atlases surface-analysis tools cerebellar cortex based on ultra-high-resolution ex vivo MRI, (2) functional connectivity gradient patterns revealed by awake resting-state fMRI, (3) structural-connectivity mapping nuclei using high-resolution diffusion MRI tractography. elucidates details cerebellum, reveals distinct intra-cerebellar connectivity, maps topological relationship As version 5 Marmoset Brain Mapping project, publicly available at https://marmosetbrainmapping.org/MBMv5.html.

Language: Английский

---

Sound localization acuity of the common marmoset (Callithrix jacchus)

Hearing Research, Journal Year: 2023, Volume and Issue: 430, P. 108722 - 108722

Published: Feb. 12, 2023

Language: Английский

---

Modeling and analysis of buffer gas effect on 52S1/2→52D5/2 and 52S1/2→72S1/2 broadened two-photon pumped Rb vapor lasers

Optics & Laser Technology, Journal Year: 2023, Volume and Issue: 171, P. 110448 - 110448

Published: Dec. 14, 2023

Language: Английский

---

Voice patches in the marmoset auditory cortex revealed by wide-field calcium imaging

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 22, 2024

ABSTRACT Species-specific vocalizations are behaviorally critical sounds. Similar to faces, species-specific important for the survival and social interactions of both humans vocal animals. Face patches have been found in brains human non-human primates. In humans, a voice patch system has identified on lateral superior temporal gurus (STG) that is selective voices over other primates, while vocalization-selective regions were rostral portion lobe outside auditory cortex macaques marmosets using functional magnetic resonance imaging (fMRI), it yet clear whether present cortex. Using wide-field calcium imaging, technique with high spatial resolution, we discovered two marmoset preferentially respond sounds carry call types identity information. One located posterior primary (A1), one anterior non-core region These functionally connected hierarchically organized as shown by latency selectivity analyses. Our findings reveal existence support notion similar cortical architectures adapted recognizing communication signals faces different primate species.

Language: Английский

---

Graph-based Cell Pattern Recognition for Merging the Multi-modal Optical Microscopic Image of Neurons

Computer Methods and Programs in Biomedicine, Journal Year: 2024, Volume and Issue: 256, P. 108392 - 108392

Published: Aug. 25, 2024

A deep understanding of neuron structure and function is crucial for elucidating brain mechanisms, diagnosing treating diseases. Optical microscopy, pivotal in neuroscience, illuminates neuronal shapes, projections, electrical activities. To explore the projection specific functional neurons, scientists have been developing optical-based multimodal imaging strategies to simultaneously capture dynamic vivo signals static ex structures from same neuron. However, original position neurons highly susceptible displacement during imaging, presenting a significant challenge integrating information at single-neuron level. This study introduces graph-model-based approach cell image matching, facilitating precise automated pairing sparsely labeled across different optical microscopic images. It has shown that utilizing distribution as matching feature can mitigate modal differences, high-order graph model address scale inconsistency, nonlinear iteration resolve discrepancies density. strategy was applied connectivity mouse visual cortex, performing between two-photon calcium HD-fMOST brain-wide anatomical sets. Experimental results demonstrate 96.67% precision, 85.29% recall rate, 90.63% F1 Score, comparable expert technicians. builds bridge structural offering technical support classification circuitry analysis.

Language: Английский

---

Marmoset Brain Mapping V5: an Anatomical and Connectivity Atlas of the Cerebellum

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 2, 2023

Summary The cerebellum is a key region of the brain for motor control and cognitive functioning, it involved bidirectional communication with cerebral cortex. As one smallest non-human primates, common marmoset provides many advantages in study anatomy functions cerebello-cerebral circuits. However, far from being well described published resources. In this study, we present comprehensive atlas three parts: 1) fine-detailed anatomical atlases surface-analyzing tools cerebellar cortex, based on ultra-high resolution ex-vivo MRI; 2) functional-connectivity gradients awake resting-state fMRI; 3) structural-connectivity mapping nuclei, high-resolution diffusion tractography. shows details cerebellum; reveals distinct gradient patterns intra-cerebellar functional connectivity; maps topological relationship nuclei version 5 Marmoset Brain Mapping project, describes connectivity publicly available via marmosetbrainmapping.org .

Language: Английский

---