Development of a Luciferase Immunosorbent Assay for Detecting Crimean–Congo Hemorrhagic Fever Virus IgG Antibodies Based on Nucleoprotein DOI Creative Commons
Qi Chen, Yuting Fang, Ning Zhang

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 32 - 32

Published: Dec. 28, 2024

Crimean–Congo hemorrhagic fever (CCHF) is a serious tick-borne disease with wide geographical distribution. Classified as level 4 biosecurity risk pathogen, CCHF can be transmitted cross-species due to its aerosol infectivity and ability cause severe outbreaks high morbidity mortality. However, current methods for detecting anti-CCHFV antibodies are limited. This study aimed develop novel luciferase immunosorbent assay (LISA) the detection of CCHFV-specific IgG antibodies. We designed specific antigenic fragments nucleoprotein evaluated their sensitivity specificity in serum samples from mice horses. In addition, we compared efficacy our LISA commercial enzyme-linked (ELISA). Our results demonstrated that optimal antigen was located within stalk cut-off domain nucleoprotein. The exhibited indicated species significantly higher (at least 128 times) ELISA. proposed CCHFV-LISA has potential facilitate serological diagnosis epidemiological investigation CCHFV natural foci, providing valuable technical support surveillance early warning this disease.

Language: Английский

Development of a Cell-Free, Toehold Switch-Based Biosensor for Rapid and Sensitive Zika Virus Detection DOI
Yongbin Chen, Wenhao Xia, Ziwei Pan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

The need for rapid and sensitive diagnostic tools is emphasized by the significant impact of infectious diseases on global health. This study presents a cell-free biosensor utilizing toehold switch technology, combined with nucleic acid sequence-based amplification (NASBA), high specificity sensitivity in Zika virus detection. switch, denovo-designed regulator gene expression, forms crux our detection system, offering versatile programmable approach to acid–based diagnostics. system based Escherichia coli extract served as platform sensor enabling real-time monitoring optimization reaction conditions minimal background leakage maximal activation efficiency. performance was rigorously evaluated through series tests, revealing that S23 demonstrated most promising effects sequence specificity. Notably, integration NASBA technology significantly enhanced sensitivity, achieving remarkable limit 2.9 aM, thus addressing intrinsic limitation switches detecting low-abundance targets. system's low cost, simplicity, adaptability various pathogens render it valuable asset health toolkit. advancement field synthetic biology, robust, sensitive, solution

Language: Английский

Citations

1
The Prevalence of Tick-Borne Encephalitis Virus in the Ticks and Humans of China from 2000 to 2023: A Systematic Review and Meta-Analysis DOI Creative Commons
Hongyu Qin,

Xiu Xin,

Qichao Tang

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(2), P. 146 - 146

Published: Feb. 8, 2025

This study presents a systematic review and meta-analysis of studies on tick-borne encephalitis virus (TBEV) prevalence in ticks human hosts China, published between 2000 2023. Extensive searches were conducted four databases—PubMed, CNKI, VIP, Wan Fang. The findings indicated overall pooled estimates TBEV infection humans 5.8% 9.0%, respectively. was 4.8% (95%CI, 4.5–7.1%) during 2000–2010 increased to 6.3% 4.7–7.8%) 2011–2023, mainly distributed Jilin (13.4%; 95%CI, 8.3–18.4%) Inner Mongolia (4.5%; 1.8–7.1%). higher Dermacentor silvarum than other tick species (8.1%; 3.2–12.9%). seroprevalence from 4.7% 2.9–6.4%) 17.6% 11.3–23.8%) 2011–2023. Simultaneously, the analysis results population characteristics showed that females, forestry workers, military personnel, farmers found be susceptible, highest noted among homemakers unemployed individuals. There certain differences populations different provinces, especially Xinjiang Heilongjiang. can provide reference for more comprehensive in-depth investigation infected with China.

Language: Английский

Citations

1
A Surrogate BSL2-compliant Infection Model Recapitulating Key Aspects of Human Marburg Virus Disease DOI Creative Commons
Wanying Yang,

Wujie Zhou,

Bo Liang

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Marburg virus disease (MVD) is a severe infectious characterized by fever and profound hemorrhage caused the (MARV), with mortality rate reaching 90%, posing significant threat to humans. MARV lies in its classification as biosafety level four (BSL-4) pathogen, which demands stringent experimental conditions substantial funding. Therefore, accessible practical animal models are urgently needed advance prophylactic therapeutic strategies for MARV. In this study, we constructed recombinant vesicular stomatitis (VSV) expressing glycoprotein (VSV-MARV/GP) utilized it surrogate induce lethal infection hamsters. Syrian hamsters infected VSV-MARV/GP presented symptoms such thrombocytopenia, lymphopenia, hemophilia, multiorgan failure, developing systemic akin that observed human patients, all animals succumbing at 2 3 days post-infection (dpi). Notably, pathogenicity of was found be species-specific, age-related, sex-associated, challenge route-dependent. Subsequently, efficacy MR191 monoclonal antibody validated model. summary, alternative model an effective tool rapidly screening medical countermeasures against GP vivo under BSL-2 conditions.

Language: Английский

Citations

0
Seeking innovative concepts in development of antiviral drug combinations DOI Creative Commons
Denis E. Kainov,

Erlend Ravlo,

Aleksandr Ianevski

et al.

Antiviral Research, Journal Year: 2025, Volume and Issue: 234, P. 106079 - 106079

Published: Jan. 9, 2025

Language: Английский

Citations

0
Assessment of In Vitro Models of the Human Buccal Mucosa for Vaccine and Adjuvant Development DOI
Madison M. Davis, Irnela Bajrovic, Maria A. Croyle

et al.

Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

To understand requirements for immunization via the oral mucosa, an in vitro model that recapitulates physical barrier of mouth, allows quantification antigen uptake and permeability mounts inflammatory response to adjuvant is needed. The structure 4 models human mucosa was determined by histochemical staining transepithelial electrical resistance (TEER) measurements. A TR146 based air-liquid interface (ALI) most closely mimicked vivo conditions. This confirmed validation studies using dextran caffeine as diffusant molecules. Apparent coefficients (Papp) adenovirus (Ad) adeno-associated virus (AAV) this were 4.3 × 10-13 2.2 10-10 respectively, while 100% total dose H1N1 influenza remained epithelial layer. Sodium glycocholate a hyperosmotic formulation improved amount Ad (p = 0.02) AAV 0.003) entered epithelium, respectively. Significant amounts IL-6 (45.1 pg/mL), GM-CSF (94.7 pg/mL) IFN-γ (4.3 produced infection. Treatment with AS03-like induced production (34.9 TNF-∝ (43 (121.2 (14.1 pg/mL). highlights contribution differentiated cells immune vaccines adjuvants.

Language: Английский

Citations

0
Perspective Chapter: Molecular Diagnostics in Viral Outbreak Surveillance DOI
Jennifer Giandhari, Amsha Viraragavan, Michelle Gordon

et al.

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Understanding and adopting various methods for monitoring viral outbreaks is required pathogen surveillance. Accurate diagnosis can play a significant role in the safe effective treatment prescribed. Africa remains burdened with host of infectious diseases, which challenges healthcare systems proper management diseases. Surveillance are implemented some parts that have infrastructure funding to perform routine testing screening. However, not all this continent equipped necessary tools support. With travel, tourism goods exchange, diseases spread across borders rapidly, posing threat global health, emphasising unified need efforts prevent, detect, act on disease through improved access vaccinations treatments. Effective response depends several elements, including laboratory capacity, skilled health staff, surveillance detect limit illnesses rapidly. Traditional molecular such as genotyping polymerase chain reaction (PCR)-based detection now being complemented like next-generation sequencing clustered regularly interspaced short palindromic repeats (CRISPR). This book chapter aims summarise current broadly describes issue climate change its impact outbreaks.

Language: Английский

Citations

0
Zoonotic Paramyxoviruses: Evolution, Ecology, and Public Health Strategies in a Changing World DOI Creative Commons
Francesco Branda, Grazia Pavia, Alessandra Ciccozzi

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(11), P. 1688 - 1688

Published: Oct. 29, 2024

The family

Language: Английский

Citations

1
Development of a Luciferase Immunosorbent Assay for Detecting Crimean–Congo Hemorrhagic Fever Virus IgG Antibodies Based on Nucleoprotein DOI Creative Commons
Qi Chen, Yuting Fang, Ning Zhang

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 32 - 32

Published: Dec. 28, 2024

Crimean–Congo hemorrhagic fever (CCHF) is a serious tick-borne disease with wide geographical distribution. Classified as level 4 biosecurity risk pathogen, CCHF can be transmitted cross-species due to its aerosol infectivity and ability cause severe outbreaks high morbidity mortality. However, current methods for detecting anti-CCHFV antibodies are limited. This study aimed develop novel luciferase immunosorbent assay (LISA) the detection of CCHFV-specific IgG antibodies. We designed specific antigenic fragments nucleoprotein evaluated their sensitivity specificity in serum samples from mice horses. In addition, we compared efficacy our LISA commercial enzyme-linked (ELISA). Our results demonstrated that optimal antigen was located within stalk cut-off domain nucleoprotein. The exhibited indicated species significantly higher (at least 128 times) ELISA. proposed CCHFV-LISA has potential facilitate serological diagnosis epidemiological investigation CCHFV natural foci, providing valuable technical support surveillance early warning this disease.

Language: Английский

Citations

0