Enhancing detection and monitoring of circulating tumor cells: Integrative approaches in liquid biopsy advances DOI Creative Commons

Thanmayi Velpula,

Buddolla Viswanath

The Journal of Liquid Biopsy, Journal Year: 2025, Volume and Issue: 8, P. 100297 - 100297

Published: April 29, 2025

Liquid biopsy offers a minimally invasive method for detecting and monitoring cancer, with key biomarkers including circulating tumor cells (CTCs), cell-free DNA (cfDNA), extracellular vesicles (EVs). Among these, CTCs provide dynamic insights into heterogeneity, metastatic potential, therapeutic resistance through molecular profiling single-cell analysis. This review examines recent advancements in technologies such as microfluidics, nanotechnology, next-generation sequencing (NGS), which have improved the accuracy clinical use of CTC detection. A comparative analysis liquid techniques highlights strengths limitations platforms, NGS, digital PCR (ddPCR), quantitative (qPCR), providing diagnostic significance. Combining genomic, transcriptomic, proteomic analyses artificial intelligence (AI) tools has enhanced supports personalized treatment decisions precision oncology. Despite notable progress, issues like assay standardization, sample variability, regulatory complexity, data integration still limit widespread use. Future directions emphasize interdisciplinary innovation, validation, robust bioinformatics frameworks to facilitate seamless incorporation CTC-based standard oncology practice. Overcoming these challenges may allow become tool early cancer detection, ongoing monitoring, individualized treatment.

Language: Английский

Traditional Chinese medicine in lung cancer treatment DOI Creative Commons
Zhichao Xi,

Rongchen Dai,

Yufei Ze

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Feb. 26, 2025

Lung cancer remains a major global health challenge and one of the leading causes cancer-related deaths worldwide. Despite significant advancements in treatment, challenges such as drug resistance, side effects, metastasis recurrence continue to impact patient outcomes quality life. In response, there is growing interest complementary integrative approaches care. Traditional Chinese medicine (TCM), with its long history, abundant clinical experience, holistic perspective individualized approach, has garnered increasing attention for role lung prevention management. This review provides comprehensive overview advances TCM covering theoretical foundation, treatment principles, experiences evidence supporting efficacy. We also provide systematic summary preclinical mechanisms, through which impacts cancer, including induction cell death, reversal inhibition modulation immune responses. Additionally, future prospects are discussed, offering insights into expanded application integration modern address this challenging disease.

Language: Английский

Citations

6
The Role of Inflammation in Cancer: Mechanisms of Tumor Initiation, Progression, and Metastasis DOI Creative Commons
Atsushi Nishida, Akira Andoh

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 488 - 488

Published: March 25, 2025

Inflammation is an essential component of the immune response that protects host against pathogens and facilitates tissue repair. Chronic inflammation a critical factor in cancer development progression. It affects every stage tumor development, from initiation promotion to invasion metastasis. Tumors often create inflammatory microenvironment induces angiogenesis, suppression, malignant growth. Immune cells within interact actively with cells, which drives progression through complex molecular mechanisms. triggered by factors such as infections, obesity, environmental toxins strongly linked increased risk. However, acute responses can sometimes boost antitumor immunity; thus, presents both challenges opportunities for therapeutic intervention. This review examines how contributes biology, emphasizing its dual role tumorigenesis potential target.

Language: Английский

Citations

4
Post-Translational Modifications of Proteins Orchestrate All Hallmarks of Cancer DOI Creative Commons
Pathea Bruno,

Aneeta Arshad,

Maria-Raluca Gogu

et al.

Life, Journal Year: 2025, Volume and Issue: 15(1), P. 126 - 126

Published: Jan. 18, 2025

Post-translational modifications (PTMs) of proteins dynamically build the buffering and adapting interface between oncogenic mutations environmental stressors, on one hand, cancer cell structure, functioning, behavior. Aberrant PTMs can be considered as enabling characteristics long they orchestrate all malignant variability in proteome cells, cancer-associated tumor microenvironment (TME). On other enhance anticancer mechanisms tumoral ecosystem or sustain beneficial effects oncologic therapies through degradation inactivation carcinogenic or/and activation tumor-suppressor proteins. In this review, we summarized analyzed a wide spectrum involved regulatory that drive tumorigenesis, genetic instability, epigenetic reprogramming, events metastatic cascade, cytoskeleton extracellular matrix (ECM) remodeling, angiogenesis, immune response, tumor-associated microbiome, metabolism rewiring most important hallmarks cancer. All develop due to proteins, which modulate gene transcription, intracellular signaling, protein size, activity, stability localization, trafficking, secretion, half-life, protein–protein interactions (PPIs). associated with exploited better understand underlying molecular heterogeneous chameleonic disease, find new biomarkers progression prognosis, personalize oncotherapies, discover targets for drug development.

Language: Английский

Citations

2
Early release of circulating tumor cells after transarterial chemoembolization hinders therapeutic response in patients with hepatocellular carcinoma DOI Creative Commons
María Lola Espejo-Cruz, Sandra González‐Rubio, J.J. Espejo

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 30, 2025

Transarterial chemoembolization (TACE) is the first-line therapeutic option for patients with intermediate-stage hepatocellular carcinoma (HCC). Tumor neovascularization allows tumor growth and may facilitate release of circulating cells (CTCs) to bloodstream after TACE. We investigated relationship between early CTCs radiological response Prospective, single-center study including HCC undergoing a first TACE from January 2019 June 2023. The IsoFlux® system was used evaluate EpCAM+ CTC counts before TACE, at day 1 (D1), 30 Radiological assessed according mRECIST criteria one month procedure. vascularity by an interventional radiologist. In all, 48 were included (age 64.2 ± 7.6 years, 14.6% women). levels increased D1 (114.0% [IQR 76.5%-178.0%], p = 0.019) normalized baseline in (76.5% 41.3%-131.8%], 0.263). Higher (p 0.009) 0.026) associated hypervascularity. Larger size [OR: 1.9 (95% CI: 1.1–3.3), 0.020] increase 5.3 1.3–21.0), 0.017] independent predictors non-response especially those hypervascular lesions. A meaningful 24 h suboptimal Future studies should role dynamics select candidates adjuvant therapy analyze their impact on long-term outcomes.

Language: Английский

Citations

2
Ultrasensitive Electrochemical Screening of Circulating Tumor Cells Over Single‐Atom Nanozyme Integrated 3D Nanoarray DOI Open Access

Peilin Wu,

Yi Li, Xun He

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Late-stage diagnosis is a major contributor to cancer mortality and thus leads increased fatality, making early detection crucial for improving survival rates. Circulating tumor cells (CTC), detectable before primary tumors become clinically apparent, have emerged as vital biomarkers the identification of aggressive cancers. Here, develop single-atom nanozyme integrated nanoarray 3D nano-biointerface ultrasensitive electrochemical screening CTCs from hepatocellular carcinoma. This cytosensor capable identifying CTC at single-cell level, achieving an impressive area under curve 0.96 in receiver operating characteristics, comparable simulated multi-indicator diagnostic strategies. strategy shows great potential non-invasive carcinoma promising be applied universally diagnosis.

Language: Английский

Citations

2
Rhythm is essential: Unraveling the relation between the circadian clock and cancer DOI
Olajumoke Ogunlusi, A. Ghosh, Mrinmoy Sarkar

et al.

Critical Reviews in Oncology/Hematology, Journal Year: 2025, Volume and Issue: unknown, P. 104632 - 104632

Published: Jan. 1, 2025

Language: Английский

Citations

1
Stem Cells in Cancer: From Mechanisms to Therapeutic Strategies DOI Creative Commons

Laurence Haddadin,

Xueqin Sun

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 538 - 538

Published: April 3, 2025

Stem cells have emerged as a pivotal area of research in the field oncology, offering new insights into mechanisms cancer initiation, progression, and resistance to therapy. This review provides comprehensive overview role stem cancer, focusing on (CSCs), their characteristics, implications for We discuss origin identification CSCs, tumorigenesis, metastasis, drug resistance, potential therapeutic strategies targeting CSCs. Additionally, we explore use normal therapy, tissue regeneration delivery vehicles anticancer agents. Finally, highlight challenges future directions cell cancer.

Language: Английский

Citations

1
DNA network-enhanced electrochemical cytosensor for analyzing mesenchymal CTCs and epithelial-mesenchymal transition in ovarian cancer DOI
Ying Deng, Kai Hu, Yuying Sun

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162728 - 162728

Published: April 1, 2025

Language: Английский

Citations

1
GRHL2-HER3 and E-cadherin mediate EGFR-bypass drug resistance in lung cancer cells DOI Creative Commons
Fumiya Ito,

Wakiko Iwata,

Yoshihiro Adachi

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 17, 2025

Epidermal growth factor receptor (EGFR) is a major oncogenic protein, and thus EGFR-targeting therapies are widely used in patients with various types of cancer, including lung cancer. However, resistance to EGFR inhibitors, such as erlotinib, presents significant challenge treating In this study, we established an EGFR-independent, erlotinib-resistant (ER) phenotype cancer A549 cells by exposing them erlotinib for extended period. The resulting ER exhibited dramatic increase resistance, decreased protein level, enhanced tumor growth, suggesting robust mechanism bypassing inhibition. RNA sequencing identified the transcription GRHL2 critical player resistance. was upregulated cells, its knockdown knockout significantly reduced Further analysis revealed that upregulates tyrosine kinase HER3, HER3 similarly decreases IC50 erlotinib. Additionally, showed increased cell-cell adhesion, linked E-cadherin. E-cadherin found be vital largely independent GRHL2, highlighting multiple parallel pathways sustaining These findings provide novel drug suggest combination targeting both GRHL2-HER3 E-cadherin-mediated may necessary overcome

Language: Английский

Citations

0
Appendiceal cancer in pregnancy and circulating tumor cells detection in the umbilical cord DOI Creative Commons

Risa Kudo,

Kensuke Matsumoto,

Tatsuya Ishiguro

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(3), P. e42411 - e42411

Published: Feb. 1, 2025

Although malignant tumors metastasizing to infant or the placenta are occasionally reported during pregnancy, adverse effects of maternal on fetus have not been clarified. Due rarity appendiceal cancer tumor pregnancy Herein, we report a 30-year-old woman who experienced (stage IVC) wherein metastatic ovarian was detected pregnancy. A bilateral salpingo-oophorectomy and partial resection omentum were performed at 25 weeks 6 days gestational age (GA), followed by Cesarean section 30 1 day GA. Postpartum chemotherapy with fluorouracil, oxaliplatin, levofolinate calcium could sufficiently control her disease. Purpura observed all over body birth. The neonatal disseminated intravascular coagulation score 4 according scoring system used Japanese Society Obstetrical, Gynecological & Neonatal Hematology, which meet criteria. Detailed histopathological examination did detect any cells in umbilical cord. Moreover, no other abnormalities found based comprehensive medical evaluation infant. However, circulating (CTCs) both cord blood samples from patient. Collectively, this case might be first step into studying circulation CTCs through via its fetal outcome; worsened condition. More advanced analysis would useful for predicting negative future.

Language: Английский

Citations

0