Deciphering factors driving soil microbial life‐history strategies in restored grasslands

iMeta, Journal Year: 2022, Volume and Issue: 2(1)

Published: Dec. 4, 2022

In macroecology, the concept of r- and K-strategy has been widely applied, yet, there have limited studies on microbial life-history strategies in temperate grasslands using multiple sequencing approaches. Total phospholipid fatty acid (PLFA) analysis, high-throughput meta-genomic sequencing, GeoChip technologies were used to examine changes traits a chronosequence restored (1, 5, 10, 15, 25, 30 years since restoration). Grassland restoration increased relative abundances Actinobacteria, Proteobacteria, Bacteroidetes but reduced Acidobacteria, Planctomycetes, Chloroflexi. PLFA analysis revealed that grassland fungi:bacteria Gram-positive:Gram-negative bacteria ratios. Combined with data, we found shifted microorganisms from oligotrophic (K-) copiotrophic (r-) groups, consistent rRNA operon copy number community. Structural equation modeling showed soil properties positively (

Language: Английский

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Microbial metabolites in the marine carbon cycle

Nature Microbiology, Journal Year: 2022, Volume and Issue: 7(4), P. 508 - 523

Published: April 1, 2022

Language: Английский

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Ecological modelling approaches for predicting emergent properties in microbial communities

Nature Ecology & Evolution, Journal Year: 2022, Volume and Issue: 6(7), P. 855 - 865

Published: May 16, 2022

Language: Английский

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Fungal community structure and function shifts with atmospheric nitrogen deposition

Global Change Biology, Journal Year: 2020, Volume and Issue: 27(7), P. 1349 - 1364

Published: Nov. 7, 2020

Abstract Fungal decomposition of soil organic matter depends on nitrogen (N) availability. This ecosystem process is being jeopardized by changes in N inputs that have resulted from a tripling atmospheric deposition the last century. Soil fungi are impacted due to higher availability, as soils acidified, or micronutrients become increasingly limiting. communities persist with chronic may be enriched traits enable them tolerate environmental stress, which trade‐off enabling decomposition. We hypothesized fungal would respond shifting community composition and functional gene abundances toward those stress but weak decomposers. sampled at seven eastern US hardwood forests where ambient varied 3.2 12.6 kg ha −1 year , five also experimental plots was simulated through fertilizer application treatments (25–50 ). responses across gradient. biomass richness increased sites low decreased high deposition. genes involved hydrolysis while oxidation decreased. One four generalized abiotic tolerance In summary, we found divergent response depended levels. biomass, richness, oxidative enzyme potential were reduced suggesting pushed beyond an threshold. structure function enrichment regional scale.

Language: Английский

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Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes

mSystems, Journal Year: 2022, Volume and Issue: 7(1)

Published: Jan. 11, 2022

Enhancing soil phosphate solubilization is a promising strategy for agricultural sustainability, while little known about the mechanisms of how microorganisms cope with differing phosphorus availability. Using combination genome-resolved metagenomics and amplicon sequencing, we investigated microbial involved in cycling under three treatments wheat-maize rotation system two natural reforestation treatments. Available was key factor shaping bacterial fungal community composition function across our sites. Membrane-bound quinoprotein glucose dehydrogenase (PQQGDH) exopolyphosphatases (PPX) governed agroecosystems. In contrast, genes encoding glycerol-3-phosphate transporters (ugpB, ugpC, ugpQ) displayed significantly greater abundance soils. The gcd gene PQQGDH found to be best determinant bioavailable phosphorus. Metagenome-assembled genomes (MAGs) affiliated Cyclobacteriaceae Vicinamibacterales were obtained from Their MAGs harbored not only but also pit low-affinity transporters. soils Microtrichales Burkholderiales. These contain ugp no gcd, thereby are indicative transporter strategy. Our study demonstrates that knowledge distinct acquisition strategies between could help linking processes cycling. IMPORTANCE microbiome player regulating processes. Identifying phosphate-solubilizing bacteria utilizing them release recalcitrant bound rocks or minerals have implications improving crop nutrient productivity. this study, combined functional sequencing analyze We differed these ecosystems. A dominated soils, observed further identified taxa contributed enhanced agroecosystem. microbes predicted beneficial increase bioavailability through practices.

Language: Английский

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Advancing the mechanistic understanding of the priming effect on soil organic matter mineralisation

Functional Ecology, Journal Year: 2022, Volume and Issue: 36(6), P. 1355 - 1377

Published: March 9, 2022

Abstract The priming effect (PE) is a key mechanism contributing to the carbon balance of soil ecosystem. Almost 100 years research since its discovery in 1926 have led rich body scientific publications identify drivers and mechanisms involved. A few review articles summarised acquired knowledge; last major one was published 2010. Since then, knowledge on microbial communities involved PE + C sequestration has been considerably renewed. This article reviews current state what extent new insights may improve our ability understand predict evolution stocks. We propose framework unify different concepts terms that emerged from international community this topic, report recent discoveries needs. Seventy per cent studies were 10 years, illustrating renewed interest for PE, probably linked increased concern about importance climate change food security issues. Among all proposed along with explain some are named differently but actually refer same object. overall introduces ‘artificial’ complexity mechanistic understanding we common, shared terminology. Despite remaining gaps, consistent progress achieved decipher abiotic underlying together role enzymes identity actors However, including into models SOM dynamics remains challenging as long not fully understood. In meantime, empirical alternatives available reproduce observations accurately when calibration robust. Based knowledge, scenarios depicting impact ecosystem services under conditions. Read free Plain Language Summary Journal blog.

Language: Английский

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Hyphae move matter and microbes to mineral microsites: Integrating the hyphosphere into conceptual models of soil organic matter stabilization

Global Change Biology, Journal Year: 2022, Volume and Issue: 28(8), P. 2527 - 2540

Published: Jan. 6, 2022

Associations between soil minerals and microbially derived organic matter (often referred to as mineral-associated or MAOM) form a large pool of slowly cycling carbon (C). The rhizosphere, immediately adjacent roots, is thought control the spatial extent MAOM formation because it dominant entry point new C inputs soil. However, emphasis on rhizosphere implicitly assumes that microbial redistribution into bulk (non-rhizosphere) soils minimal. We question this assumption, arguing extensive fungal exploration rapid hyphal turnover, from common, encourages formation. First, we summarize published estimates length density turnover rates demonstrate are high throughout rhizosphere-bulk continuum. Second, colonization surfaces common dispersal mechanism for bacteria, argue allows non-random mineral by hyphae-associated taxa. Third, these bacterial communities their hosts determine chemical deposited colonized surfaces. Collectively, our analysis demonstrates omission hyphosphere conceptual models flow overlooks key mechanisms in soils. Moving forward, there clear need spatially explicit, quantitative research characterizing environmental drivers community composition across systems, important controls over rate chemistry minerals.

Language: Английский

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The dimensionality and structure of species trait spaces

Ecology Letters, Journal Year: 2021, Volume and Issue: 24(9), P. 1988 - 2009

Published: May 20, 2021

Abstract Trait‐based ecology aims to understand the processes that generate overarching diversity of organismal traits and their influence on ecosystem functioning. Achieving this goal requires simplifying complexity in synthetic axes defining a trait space cluster species based while identifying those with unique combinations traits. However, so far, we know little about dimensionality, robustness omission structure these spaces. Here, propose unified framework synthesis across 30 datasets representing broad variety taxa, ecosystems spatial scales show common trade‐off between quality operationality appears three six dimensions. The is generally low but highly variable among datasets. We also highlight invariant scaling relationships, whatever complexity, number clusters, dominant total richness. When richness increases, saturates, whereas tend disproportionately pack richest cluster. Based results, some rules thumb build spaces estimate subsequent functional indices.

Language: Английский

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Plant domestication shapes rhizosphere microbiome assembly and metabolic functions

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: March 31, 2023

The rhizosphere microbiome, which is shaped by host genotypes, root exudates, and plant domestication, crucial for sustaining agricultural growth. Despite its importance, how domestication builds up specific microbiomes metabolic functions, as well the importance of these affected rhizobiomes relevant exudates in maintaining growth, not understood. Here, we firstly investigated bacterial fungal communities wild accessions tetraploid wheat using amplicon sequencing (16S ITS) after 9 years process at main production sites China. We then explored ecological roles exudation shaping microbiome functions integrating metagenomics genomics approaches. Furthermore, established evident linkages between morphology traits keystone taxa based on microbial culture inoculation experiments.Our results suggested that were co-shaped both genotypes status. genomes contributed more variation diversity composition than communities, whereas status exerted much stronger influences communities. In terms interkingdom association networks, destabilized network depleted abundance taxa. Moreover, found shifted from slow growing fungi dominated to fast bacteria dominated, thereby resulting a shift fungi-dominated membership with enrichment carbon fixation genes bacteria-dominated degradation genes. Metagenomics analyses further indicated cultivars possess higher function domesticated cultivars. Notably, cultivar able harness microorganism carrying N transformation (i.e., nitrification, denitrification) P mineralization pathway, inorganic fixation, organic ammonification, solubilization are recruited releasing wheat. More importantly, our metabolite-wide study contrasting functional harnessed different nutrient acquisition strategies jointly determined aboveground phenotypes. observed although wheats distinct domestication-induced recruitment led consistent growth regulation regardless status.Our indicate profoundly assembly provide evidence plants differentiated role root-associated through release sustain belowground multi-nutrient cycles These findings valuable insights into mechanisms underlying plant-microbiome interactions crop improvement sustainable agriculture. Video Abstract.

Language: Английский

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Stoichiometric regulation of priming effects and soil carbon balance by microbial life strategies

Soil Biology and Biochemistry, Journal Year: 2022, Volume and Issue: 169, P. 108669 - 108669

Published: April 15, 2022

Language: Английский

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