Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Nov. 12, 2024
The
impact
of
microplastics
on
antibiotic
resistance
has
attracted
widespread
attention.
However,
previous
studies
primarily
focused
the
effects
individual
microplastics.
In
reality,
diverse
microplastic
types
accumulate
in
soil,
and
it
remains
less
well
studied
whether
diversity
(i.e.,
variations
color,
shape
or
polymer
type)
can
be
an
important
driver
increased
gene
(ARG)
abundance.
Here,
we
employed
microcosm
to
investigate
soil
ARG
dynamics
through
metagenomic
analysis.
Additionally,
evaluated
associated
potential
health
risks
by
profiling
virulence
factor
genes
(VFGs)
mobile
genetic
elements
(MGEs).
Our
findings
reveal
that
as
increases,
there
is
a
corresponding
rise
abundance
ARGs,
VFGs
MGEs.
We
further
identified
microbial
adaptive
strategies
involving
(changed
diversity),
community
(increased
specific
microbes),
functions
(enriched
metabolic
pathways)
correlate
with
may
thus
contribute
dissemination.
Additional
global
change
factors,
including
fungicide
application
plant
reduction,
also
contributed
elevated
suggest
that,
addition
considering
contamination
levels,
crucial
monitor
ecosystems
due
their
role
driving
dissemination
multiple
pathways.
Environmental Science & Technology,
Journal Year:
2022,
Volume and Issue:
56(21), P. 14948 - 14959
Published: May 3, 2022
Plastic
particles
impact
the
propagation
of
antibiotic
resistance
genes
(ARGs)
in
environmental
media,
and
their
perturbation
on
horizontal
gene
transfer
(HGT)
ARGs
is
recognized
as
a
critical
influencing
mechanism.
However,
studies
concerning
influence
mechanisms
plastic
HGT
were
limited,
particularly
for
effect
particle
sizes
ARG
vector-associated
mechanisms.
This
study
explored
polystyrene
(PS)
with
75,
90,
100,
1000,
10000
nm
(via
transformation)
mediated
by
pUC19,
pSTV29,
pBR322
plasmids
into
Escherichia
coli
cells.
PS
≤100
impacted
transformation
ARGs,
but
large
(1000
nm)
showed
no
obvious
effects.
Effects
three
vastly
distinct.
For
pUC19
high
replication
capacities,
was
monotonously
promoted.
pSTV29
low
suppressing
effects
observed.
attributed
to
two
competing
The
enhancing
mechanism
that
direct
interaction
membrane
lipids
indirect
associated
bacterial
oxidative
stress
response
induced
pore
formation
cell
increased
permeability,
thus
plasmid
entrance.
inhibiting
interfered
inside
E.
coli,
decreasing
tranformation.
deepened
our
understanding
dissemination
contamination.
