ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)
Published: Jan. 1, 2024
Language: Английский
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: June 13, 2023
Abstract Around 50% of humankind relies on groundwater as a source drinking water. Here we investigate the age, geochemistry, and microbiology 138 samples from 95 monitoring wells (<250 m depth) located in 14 aquifers Canada. The geochemistry show consistent trends suggesting large-scale aerobic anaerobic hydrogen, methane, nitrogen, sulfur cycling carried out by diverse microbial communities. Older groundwaters, especially with organic carbon-rich strata, contain average more cells (up to 1.4 × 10 7 mL −1 ) than younger challenging current estimates subsurface cell abundances. We observe substantial concentrations dissolved oxygen (0.52 ± 0.12 mg L [mean SE]; n = 57) older groundwaters that seem support metabolisms ecosystems at an unprecedented scale. Metagenomics, isotope analyses mixing models indicate dark is produced situ via dismutation. ancient sustain productive communities highlight overlooked present past Earth.
Language: Английский
Citations
61
Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(20)
Published: May 8, 2023
Dissimilatory sulfate reduction (DSR) mediated by sulfate-reducing microorganisms (SRMs) plays a pivotal role in global sulfur, carbon, oxygen, and iron cycles since at least 3.5 billion y ago. The canonical DSR pathway is believed to be sulfide. Herein, we report phylogenetically diverse SRMs through which zero-valent sulfur (ZVS) directly generated. We identified that approximately 9% of was directed toward ZVS with S 8 as predominant product, the ratio sulfate-to-ZVS could changed SRMs’ growth conditions, particularly medium salinity. Further coculturing experiments metadata analyses revealed DSR-derived supported various ZVS-metabolizing microorganisms, highlighting this an essential component biogeochemical cycle.
Language: Английский
Citations
26
Water Research, Journal Year: 2024, Volume and Issue: 257, P. 121700 - 121700
Published: April 30, 2024
Language: Английский
Citations
9
Organic Geochemistry, Journal Year: 2025, Volume and Issue: 203, P. 104964 - 104964
Published: Feb. 22, 2025
Language: Английский
Citations
1
Annual Review of Earth and Planetary Sciences, Journal Year: 2023, Volume and Issue: 51(1), P. 493 - 519
Published: May 31, 2023
Our understanding of Earth's rock-hosted subsurface biosphere has advanced over the past two decades through collection and analysis fluids rocks from aquifers within continental oceanic crust. Improvements in cell extraction, sorting, DNA sequencing, techniques for detecting distributions activity have revealed how combination lithology, permeability, fluid mixing processes controls diversity heterogeneous distribution microbial communities fractured rock systems. However, functions most organisms, rates their growth, remain largely unknown. To mechanistically understand what physiochemical hydrological factors control biosphere, future studies are needed to characterize physiology microorganisms adapted mineral-associated growth under energy- nutrient-limited conditions. Experiments should be designed detect synergistic interactions between microorganisms, minerals, at highly variable turnover rates.▪The is controlled by variations porosity, chemical disequilibrium.▪Several imaging can sensitively biosphere.▪The poorly known.
Language: Английский
Citations
17
Journal of environmental chemical engineering, Journal Year: 2023, Volume and Issue: 11(2), P. 109413 - 109413
Published: Feb. 1, 2023
Biofouling and biocorrosion in oil pipelines are serious problems capable of causing expensive, irreparable losses. For a better understanding processes, several lines evidence required, not least experimental considerations that emulate natural conditions inside pipelines. Herein, an omics approach is used to unveil the microbial involvement deterioration electrochemical process on X52 steel with Shewanella (SW), pipeline microbiota (AK), mixed culture (AK5), latter two obtained from sludge pig cleaning Gulf Mexico. Shotgun metagenomics showed diverse communities at species level sludge: bacteria up 112, 34 archaea, 9 fungi, 23 viruses. In AK most abundant taxa were Petrotogaceae Petrotoga (31.16%) Methanomicrobiaceae (48.51%). AK5 Methylobacterium (49%) (19.49%). Concerning fungi viruses, respectively: Clavariaceae (43.26%), Picciniaceae (35.59), Onygenaceae (3.77%), Siphoviridae (46.8%), Myoviridae (33.50%), Podoviridae (7.36%) AK5. Proteomics revealed 32,284 proteins AK, 13,664 AK5, 7 SW. Expressed oxidoreductases, lyases, nitrogenases, metalloproteases, hydrolases associated process, for instance, electron transport, sulfur oxidation, nitrate reduction, biofilm formation, oxygen reduction. addition functional annotation metagenomes KEGG pathways, through Multigenomic Entropy Based Score (MEBS) metabolic functions evaluated explaining relation energy transfer processes biogeochemical cycles. The was analyzed by X-ray diffraction (XRD), fluorescence (XRF), Mössbauer spectroscopy suggesting biofouling processes. Regarding assessments, scanning microscopy (SEM) Energy Dispersive Spectroscopy (EDX) coupled SEM, SW biofilms behaved differently. Three mechanisms proposed.
Language: Английский
Citations
16
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 906, P. 167757 - 167757
Published: Oct. 16, 2023
Language: Английский
Citations
16
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: June 19, 2024
Abstract Archaea possess characteristic membrane-spanning lipids that are thought to contribute the adaptation extreme environments. However, biosynthesis of these is poorly understood. Here, we identify a radical S -adenosyl-L-methionine (SAM) enzyme synthesizes glycerol monoalkyl tetraethers (GMGTs). The enzyme, which name GMGT synthase (Gms), catalyzes formation C(sp 3 )–C(sp ) linkage between two isoprenoid chains dialkyl (GDGTs). This conclusion supported by heterologous expression gene gms from GMGT-producing species in methanogen, as well demonstration vitro activity using purified Gms enzyme. Additionally, show genes encoding putative homologs present obligate anaerobic archaea and metagenomes obtained oxygen-deficient environments, appear be absent oxic settings.
Language: Английский
Citations
5
Journal of Cleaner Production, Journal Year: 2022, Volume and Issue: 378, P. 134532 - 134532
Published: Oct. 7, 2022
Language: Английский
Citations
20
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 22, 2023
Abstract Across the tree of life, gene lengths vary, but most are no more than a few thousand base pairs in length. The largest protein often reported is ∼40,000 aa eukaryotic Titin. Even larger proteins may occur rapidly expanding set metagenome-derived sequences, their existence be obscured by assembly fragmentation. Here, we leverage genome curation to complete sequences that encode predicted up 85,804 aa. Overall, findings illuminate huge knowledge gap related giant proteins. Although >30,000 bacterial phyla such as Firmicutes and Actinobacteria , they common ca. Omnitrophota, ultra small bacteria adopt predatory lifestyles. All full length genes numerous transmembrane regions divergent secA DEAD helicase domains. In silico structural prediction subregions was required identify domains unannotated segments, revealed putative implicated attachment carbohydrate degradation. Many new near-complete Omnitrophota genomes close proximity homologous type II secretion systems well import systems. This, combination with domain content, suggests many enable prey adhesion cell wall digestion during predation.
Language: Английский
Citations
11