Trigonelline prevents high-glucose-induced endothelial-to-mesenchymal transition, oxidative stress, mitochondrial dysfunction, and impaired angiogenic activity in human endothelial EA.hy926 cells DOI Open Access

Paleerath Peerapen,

Wanida Boonmark,

Suwichaya Chantarasaka

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117320 - 117320

Published: Aug. 26, 2024

Trigonelline (TRIG) is a natural compound in an alkaloid family found diverse plants. This exerts anti-inflammatory, anti-allergic, anti-oxidative and anti-fibrotic activities several disease models. However, its beneficial role endothelial injury, especially induced by diabetes, unclear. We, therefore, evaluated the effects of TRIG on cellular proteome human (EA.hy926) cells followed functional validation high-glucose (HG)-induced deteriorations. Label-free quantification using nanoLC-ESI-Qq-TOF MS/MS revealed 40 downregulated 29 upregulated proteins TRIG. Functional enrichment analysis DAVID REVIGO tools suggested involvement these altered biological processes molecular functions, particularly cell-cell adhesion, ATP metabolic process, cell redox homeostasis, cadherin binding, hydrolysis activity. Experimental showed that HG triggered endothelial-to-mesenchymal transition (EndMT) (as demonstrated increased spindle index mesenchymal markers, i.e., fibronectin vimentin, decreased PECAM-1 VE-cadherin), oxidized proteins, reduced intracellular ATP, active mitochondria, tube/mesh formation VEGF secretion. successfully abolished all defects HG. These data indicate prevents HG-induced EndMT, oxidative stress, mitochondrial dysfunction, impaired angiogenic activity cells.

Language: Английский

Development of D–A–D‐Type NIR‐II Photothermal Agents for Synergistic Eradication of Multidrug‐Resistant Bacteria and Promoting Diabetic Wound Healing DOI
Ji Liu,

Yuxin Wang,

Weijie Gao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract The challenge in treating diabetic foot infections caused by drug‐resistant bacteria is rapidly eradicating and accelerating wound healing. NIR‐II‐induced photothermal therapy (PTT) offers notable advantages over conventional treatments, such as broad‐spectrum bactericidal efficacy, better penetration depth biological tissues, higher skin tolerance thresholds, making it particularly suitable for addressing infections. Herein, found that NIR‐II dye IR26 exhibited good effects but poor stability. By modifying with methoxy triphenylamine groups, a novel D–A–D‐type small molecule agent (IRC) developed, high stability conversion efficiency (44.3%). IRC maximum absorption wavelength of 1166 nm emission 1238 nm. However, PTT alone cannot effectively promote Therefore, nanoplatforms (Cur‐IRC@PCM) are developed coloading curcumin, natural wound–healing compound, into thermosensitive liposomes to treat MRSA‐infected wounds. Under 980 laser irradiation, Cur‐IRC@PCM provided controlled the precise release synergizing eradicate accelerate closure. intelligent antibacterial also exhibits excellent biocompatibility, rendering promising therapeutic tool biomedical fields combating bacterial

Language: Английский

Citations

11
Forsythoside A regulates pulmonary fibrosis by inhibiting endothelial-to-mesenchymal transition and lung fibroblast proliferation via the PTPRB signaling DOI Creative Commons
Qinqin Zhang, Beibei Zhang, Fan Yang

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155715 - 155715

Published: May 10, 2024

Pulmonary fibrosis (PF) is an end-stage change in many interstitial lung diseases, whereas no proven effective anti-pulmonary fibrotic treatments. Forsythoside A (FA) derived from Forsythia suspensa (Thunb.) Vahl, has been found to possess lung-protective effect. However, studies on its effect are limited and mechanism of action remains unknown.

Language: Английский

Citations

5
Fructosyl-mangiferin Ameliorates Dextran Sulfate Sodium-induced Colitis in Mice via the STAT3/M1/Th17 Axis DOI
Ping Xu,

Yuping Qian,

Xu Guo

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156475 - 156475

Published: Feb. 5, 2025

Language: Английский

Citations

0
SIRT3/6/7: promising therapeutic targets for pulmonary fibrosis DOI Creative Commons
Pingping Huang, Qin Du,

Qin Yan-ling

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 2, 2025

Pulmonary fibrosis is a chronic progressive fibrosing interstitial lung disease of unknown cause, characterized by excessive deposition extracellular matrix, leading to irreversible decline in function and ultimately death due respiratory failure multiple complications. The Sirtuin family group nicotinamide adenine dinucleotide (NAD+) -dependent histone deacetylases, including SIRT1 SIRT7. They are involved various biological processes such as protein synthesis, metabolism, cell stress, inflammation, aging through deacetylation. This article reviews the complex molecular mechanisms poorly studied SIRT3, SIRT6, SIRT7 subtypes latest research progress targeting them treat fibrosis.

Language: Английский

Citations

0
Bleomycin pollution and lung health: The therapeutic potential of peimine in bleomycin-induced pulmonary fibrosis by inhibiting glycolysis DOI Creative Commons
Kangchen Li, Xuefang Liu,

Ruilong Lu

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 289, P. 117451 - 117451

Published: Dec. 2, 2024

Language: Английский

Citations

1
Trigonelline prevents high-glucose-induced endothelial-to-mesenchymal transition, oxidative stress, mitochondrial dysfunction, and impaired angiogenic activity in human endothelial EA.hy926 cells DOI Open Access

Paleerath Peerapen,

Wanida Boonmark,

Suwichaya Chantarasaka

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117320 - 117320

Published: Aug. 26, 2024

Trigonelline (TRIG) is a natural compound in an alkaloid family found diverse plants. This exerts anti-inflammatory, anti-allergic, anti-oxidative and anti-fibrotic activities several disease models. However, its beneficial role endothelial injury, especially induced by diabetes, unclear. We, therefore, evaluated the effects of TRIG on cellular proteome human (EA.hy926) cells followed functional validation high-glucose (HG)-induced deteriorations. Label-free quantification using nanoLC-ESI-Qq-TOF MS/MS revealed 40 downregulated 29 upregulated proteins TRIG. Functional enrichment analysis DAVID REVIGO tools suggested involvement these altered biological processes molecular functions, particularly cell-cell adhesion, ATP metabolic process, cell redox homeostasis, cadherin binding, hydrolysis activity. Experimental showed that HG triggered endothelial-to-mesenchymal transition (EndMT) (as demonstrated increased spindle index mesenchymal markers, i.e., fibronectin vimentin, decreased PECAM-1 VE-cadherin), oxidized proteins, reduced intracellular ATP, active mitochondria, tube/mesh formation VEGF secretion. successfully abolished all defects HG. These data indicate prevents HG-induced EndMT, oxidative stress, mitochondrial dysfunction, impaired angiogenic activity cells.

Language: Английский

Citations

0