Trends in cancer, Journal Year: 2023, Volume and Issue: 9(4), P. 355 - 371
Published: Feb. 8, 2023
Language: Английский
Clinical Chemistry, Journal Year: 2024, Volume and Issue: 70(1), P. 81 - 89
Published: Jan. 1, 2024
Abstract Background Metastasis is the leading cause of cancer-related deaths. Most studies have focused on primary tumor or overt metastatic lesions, leaving a significant knowledge gap concerning blood-borne cancer cell dissemination, major step in cascade. Circulating cells (CTCs) blood patients with solid can now be enumerated and investigated at molecular level, giving unexpected information biology Content Here, we reviewed recent advances basic translational/clinical research CTCs as key elements Summary Findings from translational elucidated complexity process. Fully understanding this process will open new potential avenues for therapeutic diagnostic strategies to propose precision medicine all patients.
Language: Английский
Citations
8
ACS Sensors, Journal Year: 2023, Volume and Issue: 8(2), P. 406 - 426
Published: Jan. 25, 2023
Circulating tumor cells (CTCs) represent an interesting source of biomarkers for diagnosis, prognosis, and the prediction cancer recurrence, yet while they are extensively studied in oncobiology research, their diagnostic utility has not been demonstrated validated. Their scarcity human biological fluids impedes identification dangerous CTC subpopulations that may promote metastatic dissemination. In this Perspective, we discuss promising techniques could be used these cells. We first describe methods isolating patient-derived CTCs then use 3D biomimetic matrixes amplification analysis, followed by further analyses at single-cell single-molecule levels. Finally, how elucidation mechanical morphological properties using such as atomic force microscopy molecular biomarker nanopore-based detection combined future to provide patients healthcare providers with a more accurate diagnosis.
Language: Английский
Citations
21
ChemMedChem, Journal Year: 2023, Volume and Issue: 18(5)
Published: Jan. 4, 2023
Abstract Benzoxazines and benzoxazepines are nitrogen oxygen‐containing six seven‐membered benzo‐fused heterocyclic scaffolds, respectively. Benzoxazepines benzoxazines well‐known pharmacophores in pharmaceutical chemistry, which of significant interest have been extensively studied because their promising activity against various diseases including wide range anticancer activity. Several reports known for synthesizing benzoxazine benzoxazepine‐based compounds the literature. Herein this review provides a critical analysis synthetic strategies towards along with ranges activities based on these molecules that reported from 2010 onwards. This also focuses structure‐activity relationship benzoxazepine scaffolds containing bioactive describes how structural modification affects
Language: Английский
Citations
18
Journal of Circulating Biomarkers, Journal Year: 2023, Volume and Issue: 12, P. 26 - 33
Published: Aug. 7, 2023
The Parsortix® PC1 system, Food and Drug Administration (FDA) cleared for use in metastatic breast cancer (MBC) patients, is an epitope-independent microfluidic device the capture harvest of circulating tumor cells from whole blood based on cell size deformability. This report details analytical characterization linearity, detection limit, precision, reproducibility this device.System performance was determined using K2-EDTA samples collected self-declared healthy female volunteers (HVs) MBC patients spiked with prelabeled cultured lines (SKBR3, MCF7, or Hs578T). Samples were processed systems captured harvested enumerated.The system live SKBR3, Hs578T fixed SKBR3 linearly between 2 ~100 cells, average rates 69%, 73%, 79%, 90%, respectively. To ≥1 ≥95% time, required 3, 5 4 MCF7 Average precision studies 5, 10, ~50 into each line ranged 63.5% to 76.2%, repeatability percent coefficient variation (%CV) estimates ranging 12.3% 32.4% 13.3% 34.1%, ~20 HV patient 75.0% ± 16.1% (%CV = 22.3%) 68.4% 14.3% 21.1%), respectively.These evaluations demonstrate reproducibly harvests over a range 1 cells.
Language: Английский
Citations
17
Biochimie, Journal Year: 2024, Volume and Issue: 220, P. 122 - 143
Published: Jan. 3, 2024
Language: Английский
Citations
6
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 11026 - 11026
Published: July 3, 2023
Circulating tumor RNA (ctRNA) has recently emerged as a novel and attractive liquid biomarker. CtRNA is capable of providing important information about the expression variety target genes noninvasively, without need for biopsies, through use circulating sequencing. The overexpression cancer-specific transcripts increases tumor-derived signal, which overcomes limitations due to low quantities DNA (ctDNA). purpose this work present an up-to-date review current knowledge regarding ctRNAs their status biomarkers address diagnosis, prognosis, prediction, drug resistance colorectal cancer. final section article discusses practical aspects involved in analyzing plasma ctRNA, including storage isolation, detection technologies, clinical applications.
Language: Английский
Citations
16
Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(8)
Published: Aug. 17, 2023
Abstract Despite the advancements made in diagnosis and treatment of cancer, stages associated with metastasis remain largely incurable represent primary cause cancer-related deaths. The dissemination cancer is facilitated by circulating tumor cells (CTCs), which originate from or metastatic sites enter bloodstream, subsequently spreading to distant parts body. CTCs have garnered significant attention research due their accessibility peripheral blood, despite low abundance. They are being extensively studied gain a deeper understanding mechanisms underlying identify effective therapeutic strategies for advanced disease. Therefore, substantial efforts been directed towards establishing characterizing relevant experimental models derived CTCs, aiming provide tools research. In this review, we an overview recent progress establishment preclinical CTC-derived models, such as xenografts (CDX) cell cultures, show promise study CTCs. We discuss advantages limitations these conclude summarizing potential future use decisions utility precision medicine tools.
Language: Английский
Citations
16
Chemosensors, Journal Year: 2023, Volume and Issue: 11(9), P. 488 - 488
Published: Sept. 4, 2023
Electrochemical aptasensors have gained significant attention due to their exceptional sensitivity, selectivity, stability, and rapid response, combining the advantages of electrochemical techniques with specific recognition ability aptamers. This review aims provide a comprehensive summary recent advances in aptasensors. Firstly, construction method are introduced. Subsequently, highlights application progress detecting various chemical biological molecules, including metal ions, small drugs, proteins, exosomes, tumor cells, bacteria, viruses. Lastly, prospects challenges associated discussed.
Language: Английский
Citations
13
Stem Cells, Journal Year: 2023, Volume and Issue: 41(4), P. 310 - 318
Published: March 7, 2023
Cancer continues to remain a "Black Box," as there is no consensus on how it initiates, progresses, metastasizes, or recurs. Many imponderables exist about whether somatic mutations initiate cancer, do cancer stem cells (CSCs) exist, and if yes, are they result of de-differentiation originate from tissue-resident cells; why express embryonic markers, what leads metastasis recurrence. Currently, the detection multiple solid cancers through liquid biopsy based circulating tumor (CTCs) clusters, DNA (ctDNA). However, quantity starting material usually adequate only when has grown beyond certain size. We posit that pluripotent, endogenous, tissue-resident, very small embryonic-like (VSELs) in numbers all adult tissues, exit their quiescent state due epigenetic changes response various insults transform into CSCs cancer. VSELs share properties like quiescence, pluripotency, self-renewal, immortality, plasticity, enrichment side-population, mobilization, resistance oncotherapy. HrC test, developed by Epigeneres, offers potential for early using common set VSEL/CSC specific bio-markers peripheral blood. In addition, NGS studies VSELs/CSCs/tissue-specific progenitors All Organ Biopsy (AOB) test provide exomic transcriptomic information regarding impacted organ(s), type/subtype, germline/somatic mutations, altered gene expressions, dysregulated pathways. To conclude, AOB tests can confirm absence categorize rest subjects low/moderate/high risk also monitor therapy, remission,
Language: Английский
Citations
11
British Journal of Cancer, Journal Year: 2023, Volume and Issue: 128(11), P. 1981 - 1990
Published: March 17, 2023
Abstract Gastrointestinal (GI) cancers account for 35% of cancer-related deaths, predominantly due to their ability spread and generate drug-tolerant metastases. Arising from different locations in the GI system, majority metastatic malignancies colonise liver lungs. In this context, circulating tumour cells (CTCs) are playing a critical role formation new metastases, presence blood patients has been correlated with poor outcome. addition prognostic utility, prospective targeting CTCs may represent novel, yet ambitious strategy fight against metastasis. A better understanding CTC biology, mechanistic underpinnings weaknesses facilitate development previously underappreciated anti-metastasis approaches. Here, along related clinical studies, we outline selection literature describing biological features an impact on metastasis forming cancers.
Language: Английский
Citations
11