Identification of PANoptosis-Related Genes in Community-Acquired Pneumonia Diagnosis DOI Creative Commons

Qiaoxin Hao,

Wei Gao,

Pei Zhang

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 10289 - 10304

Published: Dec. 1, 2024

Background: This study aimed to identify and characterize novel PANoptosis biomarkers for community-acquired pneumonia (CAP) diagnosis. Methods: Transcriptomic data from training set GSE196399 validation sets GSE94916 GSE202947 were utilized. A gene was identified by intersecting DEGs linked CAP, WGCNA hub genes, PANoptosis-related genes. GO KEGG analyses conducted enrichment analysis. scores calculated via ssGSEA. Feature genes using SVM-RFE, LASSO regression, RF methods. Diagnostic performance assessed ROC Immune cell infiltration evaluated CIBERSORT. PPI network constructed, a nomogram developed CAP prediction. Drug-gene interactions investigated. qRT-PCR confirm feature alterations in clinical samples. Results: We 7555 associated with the dataset. Through WGCNA, of 39 found, showing significant pathways related apoptosis inflammation. patients exhibited significantly reduced compared healthy controls, marked upregulation majority high-score individuals. Four ( ZNF304, AKT3, MAPK8 , ARHGAP10 ) as potential biomarkers, exhibiting high diagnostic accuracy AUCs generally above 0.8. These also showed correlations M0 macrophages neutrophils. interaction analysis revealed therapeutic agents targeting AKT3 . Validation samples confirmed expression patients. Conclusion: The demonstrate serving targets CAP. Keywords: pneumonia, PANoptosis, diagnosis, neutrophil infiltration, machine learning,

Language: Английский

Ironing out the role of ferroptosis in immunity DOI Creative Commons
Hannah N. Bell, Brent R. Stockwell, Weiping Zou

et al.

Immunity, Journal Year: 2024, Volume and Issue: 57(5), P. 941 - 956

Published: May 1, 2024

Ferroptosis is a type of regulated cell death that drives the pathophysiology many diseases. Oxidative stress detectable in types death, but only ferroptosis involves lipid peroxidation and iron dependency. originates propagates from several organelles, including mitochondria, endoplasmic reticulum, Golgi, lysosomes. Recent data have revealed immune cells can both induce undergo ferroptosis. A mechanistic understanding how regulates immunity critical to controls responses this dysregulated disease. Translationally, more work needed produce ferroptosis-modulating immunotherapeutics. This review focuses on role immune-related diseases, infection, autoimmune cancer. We discuss immunity, regulation contributes disease pathogenesis, targeting may lead novel therapies.

Language: Английский

Citations

53
The role of programmed cell death in organ dysfunction induced by opportunistic pathogens DOI Creative Commons
Wang Yangyanqiu, Li Weng, Xunyao Wu

et al.

Critical Care, Journal Year: 2025, Volume and Issue: 29(1)

Published: Jan. 24, 2025

Sepsis is a life-threatening condition resulting from pathogen infection and characterized by organ dysfunction. Programmed cell death (PCD) during sepsis has been associated with the development of multiple dysfunction syndrome (MODS), impacting various physiological systems including respiratory, cardiovascular, renal, neurological, hematological, hepatic, intestinal systems. It well-established that infections lead to immune dysregulation, which subsequently contributes MODS in sepsis. However, recent evidence suggests sepsis-related opportunistic pathogens can directly induce failure promoting PCD parenchymal cells each affected organ. This study provides an overview damaged induction host pathogens, proposing innovative strategies for preventing

Language: Английский

Citations

2
Alterations in microbiota of patients with COVID‐19: implications for therapeutic interventions DOI Creative Commons
Yong Qiu, Chunheng Mo, Lu Chen

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(4)

Published: March 15, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recently caused a global pandemic, resulting in more than 702 million people being infected and over 6.9 deaths. Patients with disease (COVID-19) may suffer from diarrhea, sleep disorders, depression, even cognitive impairment, which is associated long COVID during recovery. However, there remains no consensus on effective treatment methods. Studies have found that patients COVID-19 alterations microbiota their metabolites, particularly the gut, be involved regulation of immune responses. Consumption probiotics alleviate discomfort by inflammation oxidative stress. pathophysiological process underlying alleviation COVID-19-related symptoms complications targeting unclear. In current study, we summarize latest research evidence together SARS-CoV-2 vaccine use, focus relationship between use. This work provides probiotic-based interventions improve regulating gut systemic immunity. Probiotics also used as adjuvants to efficacy.

Language: Английский

Citations

7
A deep insight into ferroptosis in lung disease: facts and perspectives DOI Creative Commons
Fan Zhang, Xiang Yu, Qiao Ma

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: March 18, 2024

In the last decade, ferroptosis has received much attention from scientific research community. It differs other modes of cell death at morphological, biochemical, and genetic levels. Ferroptosis is mainly characterized by non-apoptotic iron-dependent caused lipid peroxide excess accompanied abnormal iron metabolism oxidative stress. recent years, more studies have shown that closely related to occurrence development lung diseases. COPD, asthma, injury, fibrosis, cancer, infection respiratory diseases become third most common chronic worldwide, bringing serious economic psychological burden people around world. However, exact mechanism which involved in progression not been fully revealed. this manuscript, we describe ferroptosis, targeting signaling pathways proteins, summarize relationship between diseases, explore intervention targeted therapy for

Language: Английский

Citations

7
Bletilla striata polysaccharides protect against ARDS by modulating the NLRP3/caspase1/GSDMD and HMGB1/TLR4 signaling pathways to improve pulmonary alveolar macrophage pyroptosis DOI
Qian Wu,

Mingyuan Zhou,

Yu‐Chi Chen

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 319, P. 117361 - 117361

Published: Oct. 28, 2023

Language: Английский

Citations

9
A glimpse into viral warfare: decoding the intriguing role of highly pathogenic coronavirus proteins in apoptosis regulation DOI Creative Commons

Leyi Cheng,

Yajuan Rui, Yanpu Wang

et al.

Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 31(1)

Published: July 13, 2024

Coronaviruses employ various strategies for survival, among which the activation of endogenous or exogenous apoptosis stands out, with viral proteins playing a pivotal role. Notably, highly pathogenic coronaviruses such as SARS-CoV-2, SARS-CoV, and MERS-CoV exhibit greater array non-structural compared to low-pathogenic strains, facilitating their ability induce via multiple pathways. Moreover, these are adept at dampening host immune responses, thereby bolstering replication persistence. This review delves into intricate interplay between apoptosis, systematically elucidating molecular mechanisms underpinning induction by proteins. Furthermore, it explores potential therapeutic avenues stemming from inhibition antiviral agents utilization apoptosis-inducing modalities. These insights not only shed light on pathogenesis but also offer novel perspectives cancer therapy.

Language: Английский

Citations

3
Longitudinal transcriptomic analysis reveals persistent enrichment of iron homeostasis and erythrocyte function pathways in severe COVID-19 ARDS DOI Creative Commons
Moemen Eltobgy, Finny Johns, Daniela Farkas

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 5, 2024

Introduction The acute respiratory distress syndrome (ARDS) is a common complication of severe COVID-19 and contributes to patient morbidity mortality. ARDS heterogeneous caused by various insults, results in hypoxemic failure. Patients with from may represent subgroup patients distinct molecular profiles that drive disease outcomes. Here, we hypothesized longitudinal transcriptomic analysis identify dynamic pathobiological pathways during ARDS. Methods We identified cohort an existing ICU biorepository established three groups for comparison: 1) survived hospitalization (COVID survivors, n = 4), 2) did not survive non-survivors, 5), 3) other causes as control group (ARDS controls, 4). RNA was isolated peripheral blood mononuclear cells (PBMCs) at 4 time points (Days 1, 3, 7, 10 following admission) analyzed bulk sequencing. Results first compared transcriptomes between individual timepoints observed significant heterogeneity differentially expressed genes (DEGs). Next, utilized the likelihood ratio test exhibit different patterns change over 3 341 DEGs across time, including hemoglobin subunit alpha 2 ( HBA1, HBA2 ), beta HBB von Willebrand factor C EGF domains VWCE carbonic anhydrase 1 CA1 which all demonstrated persistent upregulation COVID non-survivors survivors. Of DEGs, 314 similar pattern increased gene expression associated canonical iron homeostasis signaling, erythrocyte interaction oxygen carbon dioxide, erythropoietin heme biosynthesis, metabolism porphyrins, uptake transport. Discussion These findings describe differences regulation course survivors multiple suggest red cell contribute This approach generalizable larger cohorts supports sampling profiling studies, novel targetable injury resolution.

Language: Английский

Citations

3
Circulating and Urinary Concentrations of Malondialdehyde in Aging Humans in Health and Disease: Review and Discussion DOI Creative Commons
Dimitrios Tsikas, Stefanos A. Tsikas, Marie Mikuteit

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(10), P. 2744 - 2744

Published: Oct. 10, 2023

(1) Background: Malondialdehyde (MDA) is a major and stable product of oxidative stress. MDA circulates in the blood excreted urine its free conjugated forms, notably with L-lysine L-serine. most frequently measured biomarker stress, namely lipid peroxidation. Oxidative stress generally assumed to be associated disease increase age. Here, we review discuss literature concerning circulating excretory as peroxidation aging subjects regard health disease, such kidney erectile dysfunction, COVID-19. (2) Methods: Scientific articles, those reporting on (plasma, serum) urinary MDA, which concern appeared PubMed were considered; they formed basis for evaluating potential particularly peroxidation, humans (3) Results Conclusions: The results reported thus far are contradictory. articles considered present study not supportive general view that increases aging. Many functions several organs, including filtration efficiency kidneys, physiologically reduced men women This effect likely result apparent “accumulation” biomarkers concomitantly an organ’s function, creatinine. How forms transported various organs (including brain) how via known, investigating these questions should objective forthcoming studies. age- gender-related creatinine might useful factor taken into consideration when

Language: Английский

Citations

8
BDNF-altering cell death mechanisms of brain disorders: pyroptosis and/or ferroptosis?: a systematic review DOI Creative Commons
Amirul Hafiz Ahmad Abdullah, Nurul Farhana Jufri, Siti Fathiah Masre

et al.

Turkish Journal of Biochemistry, Journal Year: 2024, Volume and Issue: 49(4), P. 471 - 483

Published: Aug. 1, 2024

Abstract The brain plays a central role in controlling and regulating bodily activities. Given its significance, the needs to remain optimal condition function properly. Neurodegenerative diseases arise when mechanisms maintaining homeostasis fail, resulting prolonged uncontrolled activation of cell death pathways. Increasingly, researchers are focusing on specific types death, such as ferroptosis pyroptosis, due their potential targets for preventing treating neurodegenerative diseases. Brain-derived neurotrophic factor (BDNF) is crucial growth neurogenesis, neuronal survival, maintenance, thus implicated mechanisms. This review aims elucidate BDNF pyroptosis that impact health. To achieve this, three databases – PubMed, Scopus, Web Science were searched relevant studies, yielding 95 articles, which only 14 aligned with study’s aim. Ferroptosis (iron-dependent death) (inflammatory distinct modes death; however, this found both associated oxidative stress. Consequently, findings suggest collectively health conditions depression, cognitive deficiencies, anxiety, notably linked reduced levels.

Language: Английский

Citations

2
Effects of Natural Products on Enzymes Involved in Ferroptosis: Regulation and Implications DOI Creative Commons
Hua‐Li Zuo, Hsi‐Yuan Huang, Yang-Chi-Dung Lin

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(23), P. 7929 - 7929

Published: Dec. 4, 2023

Ferroptosis is a form of regulated cell death that characterized by the accumulation iron-dependent lipid peroxides. The regulation ferroptosis involves both non-enzymatic reactions and enzymatic mechanisms. Natural products have demonstrated potential effects on various enzymes, including GPX4, HO-1, NQO1, NOX4, GCLC, GCLM, which are mainly involved in glutathione metabolic pathway or oxidative stress regulation, ACSL3 ACSL4, participate metabolism, thereby influencing ferroptosis. In this review, we provided comprehensive overview existing literature pertaining to natural enzymes discussed their implications for prevention treatment ferroptosis-related diseases. We also highlight challenge majority research has concentrated investigating impact expression involving while limited attention given enzyme activity. This observation underscores considerable scope exploring influence

Language: Английский

Citations

6