Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 12, 2025
Trypsin
is
a
specific
and
reliable
diagnostic
biomarker
of
pancreatic
function
pathological
changes.
Thus,
detecting
trypsin
screening
inhibitors
are
highly
important
for
clinical
diagnosis
disease
treatment.
Herein,
we
propose
colorimetric/fluorescence
sensing
probe
based
on
carbon
quantum
dots
(O-CDs)
cytochrome
C
(Cyt
C)
sensitive
detection
inhibitor
screening.
The
prepared
O-CDs
exhibit
persistent
fluorescence
environmental
tolerance.
In
the
presence
trypsin,
Cyt
hydrolyzed
into
heme
with
oxidase
mimetic
activity
that
can
convert
colorless
TMB
blue
oxTMB,
which
strongly
absorbs
at
652
nm
quenches
580
via
inner
filter
effect.
This
realizes
precise
determination
wide
linear
range
(10-2000
ng/mL
in
both
colorimetric
modes)
low
limit
(0.05
mode
3.44
mode).
developed
method
was
then
applied
to
detect
human
serum
urine
good
accuracy
reproducibility.
Furthermore,
successfully
screen
from
legumes
basis
their
ability
inhibit
activity.
this
dual-mode
has
great
potential
application
trypsin-related
diseases,
as
well
drugs.
MedComm,
Journal Year:
2024,
Volume and Issue:
5(9)
Published: Sept. 1, 2024
Abstract
Cell
death
regulation
is
essential
for
tissue
homeostasis
and
its
dysregulation
often
underlies
cancer
development.
Understanding
the
different
pathways
of
cell
can
provide
novel
therapeutic
strategies
battling
cancer.
This
review
explores
several
key
mechanisms
apoptosis,
necroptosis,
autophagic
death,
ferroptosis,
pyroptosis.
The
research
gap
addressed
involves
a
thorough
analysis
how
these
be
precisely
targeted
therapy,
considering
tumor
heterogeneity
adaptation.
It
delves
into
genetic
epigenetic
factors
signaling
cascades
like
phosphatidylinositol
3‐kinase/protein
kinase
B/mammalian
target
rapamycin
(PI3K/AKT/mTOR)
mitogen‐activated
protein
kinase/extracellular
signal‐regulated
(MAPK/ERK)
pathways,
which
are
critical
death.
Additionally,
interaction
microenvironment
with
cells,
particularly
influence
hypoxia,
nutrient
deprivation,
immune
cellular
interactions,
explored.
Emphasizing
strategies,
this
highlights
emerging
modulators
inducers
such
as
B
lymphoma
2
(BCL2)
homology
domain
3
(BH3)
mimetics,
tumour
necrosis
factor‐related
apoptosis‐inducing
ligand
(TRAIL),
chloroquine,
innovative
approaches
to
induce
ferroptosis
provides
insights
therapy's
future
direction,
focusing
on
multifaceted
circumvent
drug
resistance.
examination
evolving
underlines
considerable
clinical
potential
continuous
necessity
in‐depth
exploration
within
scientific
domain.
Animals,
Journal Year:
2025,
Volume and Issue:
15(3), P. 381 - 381
Published: Jan. 28, 2025
Cytochrome
c
is
a
critical
protein
in
energy
metabolism,
and
its
structural
adaptations
to
different
temperatures
play
key
role
enabling
species
like
the
wild
Bactrian
camel
(Camelus
ferus)
Arabian
dromedarius)
thrive
their
respective
cold
hot
environments.
This
study
investigates
structural,
thermodynamic,
dynamic
properties
of
cytochrome
at
temperatures.
Thermal
Titration
Molecular
Dynamics
(TTMD)
simulations,
which
involve
analyzing
behaviour
across
range
temperatures,
were
carried
out
using
GROMACS,
with
each
simulation
running
for
100
nanoseconds,
245
K,
280
303
308
320
evaluate
stability
flexibility.
Structural
alterations
indicated
by
an
increase
root
mean
square
deviations
(RMSDs)
0.4
nm
as
opposed
lower
RMSD
values
(0.1–0.2
nm)
K
K.
Root
fluctuation
(RMSF)
analyses
revealed
modest
flexibility
but
considerable
(0.3–0.4
Principal
component
analysis
(PCA)
found
that
formational
space
was
constrained
expanded
higher
Entropy
peaked
(13,816
J/mol)
then
fell
substantially
(451.765
J/mol),
indicating
diminished
stability.
These
findings
highlight
Camelus
ferus
thermal
resilience
dromedarius,
showing
evolutionary
strategies
harsh
conditions.
Frontiers in Pharmacology,
Journal Year:
2024,
Volume and Issue:
15
Published: Aug. 20, 2024
Mitochondria
play
a
significant
and
varied
role
in
inflammatory
lung
disorders.
Mitochondria,
known
as
the
powerhouse
of
cell
because
their
producing
energy,
are
now
recognized
crucial
regulators
inflammation
immunological
responses.
Asthma,
chronic
obstructive
pulmonary
disease,
acute
respiratory
distress
syndrome
characterized
by
complex
interactions
between
immune
cells,
substances,
tissue
damage.
Dysfunctional
mitochondria
can
increase
generation
reactive
oxygen
species
(ROS),
triggering
pathways.
Moreover,
mitochondrial
failure
impacts
cellular
signaling,
which
turn
affects
expression
molecules
that
promote
inflammation.
In
addition,
have
controlling
behavior
such
activation
differentiation,
is
essential
development
diseases.
Their
dynamic
behavior,
encompassing
fusion,
fission,
mitophagy,
also
responses
to
oxidative
stress.
Gaining
comprehensive
understanding
intricate
correlation
order
develop
accurate
treatment
strategies.
Targeting
ROS
generation,
dynamics,
function
may
offer
novel
approaches
treating
diseases
while
minimizing
Additional
investigation
into
precise
contributions
will
provide
knowledge
regarding
disease
mechanisms
potential
therapeutic
approaches.
This
review
focus
on
how
regulate
these
processes
involvement
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Sept. 9, 2024
Ferroptosis
is
a
type
of
cell
death
that
plays
remarkable
role
in
the
growth
and
advancement
malignancies
including
hepatocellular
carcinoma
(HCC).
Non-coding
RNAs
(ncRNAs)
have
considerable
impact
on
HCC
by
functioning
as
either
oncogenes
or
suppressors.
Recent
research
has
demonstrated
non-coding
ability
to
control
ferroptosis
cells,
hence
impacting
tumors
resistance
these
cells
drugs.
Autophagy
mechanism
conserved
throughout
evolution
maintaining
balance
body
under
normal
settings.
Nevertheless,
occurrence
dysregulation
autophagy
evident
progression
various
human
disorders,
specifically
cancer.
dual
roles
cancer,
potentially
influencing
both
survival
death.
prevalent
kind
liver
genetic
mutations
changes
molecular
pathways
might
worsen
its
advancement.
The
subject
debate,
it
capacity
repress
promote
tumor
growth.
activation
can
apoptosis,
proliferation
glucose
metabolism,
facilitate
spread
through
EMT.
Inhibiting
hinder
enhance
respond
treatment.
regulated
several
signaling
pathways,
such
STAT3,
Wnt,
miRNAs,
lncRNAs,
circRNAs.
Utilizing
anticancer
drugs
target
may
advantageous
implications
for
efficacy
cancer
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 12, 2024
Abstract
Proteins
have
emerged
as
promising
therapeutics
in
oncology
due
to
their
great
specificity.
Many
treatment
strategies
are
developed
based
on
protein
biologics,
such
immunotherapy,
starvation
therapy,
and
pro‐apoptosis
while
some
biologics
entered
the
clinics.
However,
clinical
translation
is
severely
impeded
by
instability,
short
circulation
time,
poor
transmembrane
transportation,
immunogenicity.
Micro‐
nano‐particles‐based
drug
delivery
platforms
designed
solve
those
problems
enhance
therapeutic
efficacy.
This
review
first
summarizes
different
types
of
proteins
research
stages,
highlighting
administration
limitations.
Next,
various
micro‐
nano‐particles
described
demonstrate
how
they
can
overcome
The
potential
then
explored
efficacy
combinational
therapies.
Finally,
challenges
future
directions
carriers
discussed
for
optimized
delivery.
