LncRNA NCK1-AS1 Aggravates Hepatocellular Carcinoma by the miR-22-3p/YARS Axis to Activate PI3K/AKT Signaling DOI Open Access

Weixin Zhou,

Jie Wang, Jie Zhang

et al.

Journal of Gastrointestinal and Liver Diseases, Journal Year: 2022, Volume and Issue: 31(1), P. 48 - 59

Published: March 19, 2022

Background: Hepatocellular carcinoma (HCC) is frequently diagnosed at late stages when curative treatments are no more appliable. Many studies have proved the active role of long non-coding RNAs (lncRNAs) in cancers’ biology; here, functional lncRNA NCK1-AS1 HCC was identified.Methods: Gene expression tumor tissues evaluated by examining online databases and 88 collected samples from our hospital. The interactions miR-22-3p with tyrosyl-tRNA synthetase (YARS) were tested conducting bioinformatics analysis, luciferase report, RNA pulldown experiments. CCK-8, colony formation, flow cytometry, wound healing, transwell experiments used to dissect NCK1-AS1/miR-22-3p/YARS axis HCC.Results: overexpressed cells tissues. Functional assays depicted that depletion hampered malignant character cells. controlled availability miR-22-3p, resulting YARS upregulation. found a clinical value for diagnosis. Moreover, rescue revealed inhibition or overexpression partially blocked function deficiency As downstream signaling pathway, we discovered activated PI3K/AKT miR-22-3p/YARS axis.Conclusion: present study verified could promote progression via activate signaling.

Language: Английский

Long non-coding RNA mediated drug resistance in breast cancer DOI
Deepshikha Singh, Yehuda G. Assaraf, Rajesh N. Gacche

et al.

Drug Resistance Updates, Journal Year: 2022, Volume and Issue: 63, P. 100851 - 100851

Published: July 1, 2022

Language: Английский

Citations

96
Exosomal lncRNA TUG1 from cancer-associated fibroblasts promotes liver cancer cell migration, invasion, and glycolysis by regulating the miR-524-5p/SIX1 axis DOI Creative Commons
Le Lü, Jingjing Huang,

Jiantao Mo

et al.

Cellular & Molecular Biology Letters, Journal Year: 2022, Volume and Issue: 27(1)

Published: Feb. 22, 2022

Increasing evidence suggests that taurine upregulated gene 1 (TUG1) is crucial for tumor progression; however, its role in hepatocellular carcinoma (HCC) and the underlying mechanisms are not well characterized.The expression levels of TUG1, miR-524-5p, sine oculis homeobox homolog (SIX1) were determined using quantitative real-time PCR. The regulatory relationships confirmed by dual-luciferase reporter assay. Cell proliferation invasion assessed Counting Kit 8 transwell assays. Glucose uptake, cellular lactate, lactate dehydrogenase (LDH), adenosine triphosphate (ATP) detected commercially available kits. Silencing TUG1 or SIX1 was performed lentivirus transduction. Protein measured immunoblotting.Cancer-associated fibroblasts (CAFs)-secreted exosomes promoted migration, invasion, glycolysis HepG2 cells releasing TUG1. promotive effects CAFs-secreted attenuated silencing targets miR-524-5p. knockdown inhibited miR-524-5p inhibitor. suppressed growth lung metastasis therefore increased survival xenograft model mice. We also found HCC patients with while decreased metastasis.CAFs-derived exosomal via miR-524-5p/SIX1 axis. These findings may help establish foundation development therapeutics strategies clinical management future.

Language: Английский

Citations

95
Role of lncRNAs in hepatocellular carcinoma DOI

Smriti Verma,

Bidya Dhar Sahu, Madhav Nilakanth Mugale

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 325, P. 121751 - 121751

Published: May 9, 2023

Language: Английский

Citations

26
LncRNA CRNDE Drives the Progression of Hepatocellular Carcinoma by inducing the Immunosuppressive Niche DOI Creative Commons
Xianying Li,

Shuhang Liang,

Mingming Fei

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(2), P. 718 - 732

Published: Jan. 1, 2024

As a crucial protumorigenic long noncoding RNA, colorectal tumor differential expression (CRNDE) has been confirmed to facilitate the progression of various cancers.However, its role in microenvironment (TME) hepatocellular carcinoma (HCC) is still unclear.Here we determined that CRNDE was upregulated HCC samples and CRNDE-positive cells were predominantly enriched malignant cells.In vivo functional assays revealed CRNDE-induced supported progression, recruited abundant granulocyte myeloid-derived suppressor (G-MDSCs) restricted infiltration T terms mechanisms, bound with Toll-like receptor 3 (TLR3) activated NF-κB signaling increase secretion c-x-c motif chemokine ligand (CXCL3).CRNDE knockdown could significantly suppress accumulation G-MDSCs enhance TME vivo.Taken together, our study reveals CRNDE-NF-κB-CXCL3 axis plays driving immunosuppressive niche by recruiting G-MDSCs.

Language: Английский

Citations

9
Long Non-Coding RNAs, Cell Cycle, and Human Breast Cancer DOI
Qinan Yin,

Haodi Ma,

Gayan Bamunuarachchi

et al.

Human Gene Therapy, Journal Year: 2023, Volume and Issue: 34(11-12), P. 481 - 494

Published: May 27, 2023

The long non-coding RNAs (lncRNAs) constitute an important class of the human transcriptome. discovery lncRNAs provided one many unexpected results post-genomic era and uncovered a huge number previously ignored transcriptional events. In recent years, are known to be linked with diseases, particular focus on cancer. Growing evidence has indicated that dysregulation in breast cancer (BC) is strongly associated occurrence, development, progress. Increasing numbers have been found interact cell cycle progression tumorigenesis BC. can exert their effect as tumor suppressor or oncogene regulate development through direct indirect regulation cancer-related modulators signaling pathways. What more, excellent candidates for promising therapeutic targets BC due features high tissue cell-type specific expression. However, underlying mechanisms still remain largely undefined. Here, we concisely summarize sort out current understanding research progress relationships roles lncRNA regulating cycle. We also aberrant expression BC, potential improve therapy discussed. Together, considered exciting whose altered impede progression.

Language: Английский

Citations

18
Long non‑coding RNA MKLN1‑AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR‑654‑3p, thereby promoting hepatoma‑derived growth factor expression DOI Creative Commons

Wanjun Gao,

Xiaohua Chen, Wei Chi

et al.

International Journal of Molecular Medicine, Journal Year: 2020, Volume and Issue: unknown

Published: Sept. 9, 2020

Long non‑coding RNAs (lncRNAs) have recently gained attention due to their important roles in human cancer types, such as breast and gastric cancer. The present study measured alterations muskelin 1 antisense RNA (MKLN1‑AS) expression hepatocellular carcinoma (HCC) evaluated its clinical value patients with HCC. Additionally, the current investigated effects of MKLN1‑AS on malignant features HCC cells. detailed molecular mechanisms underlying cancer‑promoting activities cells were also elucidated. tissues cell lines was detected using reverse‑transcription quantitative PCR (RT‑qPCR). Cell Counting Kit‑8 assays flow cytometry used determine proliferation apoptosis. Migration invasion assays, well tumor xenograft experiments conducted analyze migration vitro growth vivo, respectively. interaction among microRNA‑654‑3p (miR‑654‑3p), hepatoma‑derived factor (HDGF) luciferase reporter assay, immunoprecipitation RT‑qPCR, western blotting rescue experiments. upregulated lines, a high associated shorter overall survival disease‑free Functionally, knockdown impaired proliferation, invasion, induced apoptosis vitro. Knockdown inhibited vivo. results indicated that functioned competing endogenous by sponging miR‑654‑3p targeting HDGF positively modulated MKLN1‑AS, partially abrogated this effect. Rescue demonstrated overexpression both abolished knockdown‑induced cellular processes In summary, pro‑oncogenic during progression serving sponge for increase expression. Therefore, MKLN1‑AS/miR‑654‑3p/HDGF axis may offer novel target diagnosis, prognosis, prevention treatment

Language: Английский

Citations

47
LncRNA FAM83A-AS1 promotes ESCC progression by regulating miR-214/CDC25B axis DOI Creative Commons

Jinlin Jia,

Hongle Li, Jie Chu

et al.

Journal of Cancer, Journal Year: 2021, Volume and Issue: 12(4), P. 1200 - 1211

Published: Jan. 1, 2021

Background: Recent researches have pinpointed that long non-coding RNA (lncRNA) was tightly related to the carcinogenesis.However, function of lncRNA in esophageal cell squamous carcinoma (ESCC) remains be explored.In current study, we assessed expression pattern and biological FAM83A-AS1 ESCC.Methods: qRT-PCR used detect FAM83A-AS1, miR-214, CDC25B ESCC tissues lines.CCK-8, transwell, apoptosis cycle assays were performed define cells.Furthermore, regulation miR-214 by defined rescue assays.In addition, association between CDC25B, confirmed qRT-PCR.Results: Here, discovered strongly expressed tissues.FAM83A-AS1 abundance associated with TNM stages differentiation grade patients.The receiver operating characteristic curve (ROC) analysis indicated high accuracy diagnosis.Functionally, inhibiting repressed proliferation, migration, invasion ESCC.In found accelerated while inhibited apoptosis.Mechanistically, regulated expression, there a negative correlation ESCC.Rescue assay could impair suppressing effect migration induced depletion.Furthermore, direct target enhanced positively ESCC.Conclusions: Collectively, concluded facilitated progression regulating miR-214/CDC25B axis.Our study showed may act as promising for diagnosis therapy.

Language: Английский

Citations

36
A new strategy for overcoming drug resistance in liver cancer: Epigenetic regulation DOI Open Access
Xiaorui Su,

Yuxuan Li,

Yupeng Ren

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116902 - 116902

Published: June 12, 2024

Drug resistance in hepatocellular carcinoma has posed significant obstacles to effective treatment. Recent evidence indicates that, addition traditional gene mutations, epigenetic recoding plays a crucial role HCC drug resistance. Unlike irreversible changes are reversible, offering promising avenue for preventing and overcoming liver cancer. This review focuses on various modifications relevant their underlying mechanisms. Additionally, we introduce current clinical drugs trials of these as regulators other solid tumors. Although there is no study prevent the occurrence cancer, development liquid biopsy technologies provided bridge achieve this goal.

Language: Английский

Citations

6
lncRNA-CRNDE regulates BMSC chondrogenic differentiation and promotes cartilage repair in osteoarthritis through SIRT1/SOX9 DOI
Chengdi Shi, Wenhao Zheng, Jinwu Wang

et al.

Molecular and Cellular Biochemistry, Journal Year: 2021, Volume and Issue: 476(4), P. 1881 - 1890

Published: Jan. 21, 2021

Language: Английский

Citations

32
Pan-cancer characterization of long non-coding RNA and DNA methylation mediated transcriptional dysregulation DOI
Zhen Yang, Feng Xu, Haizhou Wang

et al.

EBioMedicine, Journal Year: 2021, Volume and Issue: 68, P. 103399 - 103399

Published: May 24, 2021

Language: Английский

Citations

31