Screening crucial lncRNAs and genes in osteoarthritis by integrated analysis DOI Creative Commons
Jun Wang, Yumin Zhang, Tao Ma

et al.

Advances in Rheumatology, Journal Year: 2023, Volume and Issue: 63(1)

Published: Feb. 27, 2023

Osteoarthritis (OA) is one of the most frequent chronic diseases with high morbidity worldwide, marked by degradation cartilage and bone, joint instability, stiffness, space stenosis subchondral sclerosis. Due to elusive mechanism osteoarthritis (OA), we aimed identify potential markers for OA explore molecular mechanisms underlying OA.Expression profiles data were collected from Gene Expression Omnibus database differentially expressed mRNAs (DEmRNAs) lncRNAs (DElncRNAs) in OA. Functional annotation protein-protein interaction (PPI) networks performed. Then, nearby DEmRNAs DElncRNAs was obtained. Moreover, GO KEGG pathway enrichment analysis Finally, expression validation selected performed quantitative reverse transcriptase-polymerase chain reaction.In total, 2080 664 determined PI3K-Akt signaling pathway, Endocytosis Rap1 significantly enriched pathways YWHAB, HSPA8, NEDD4L SH3KBP1 four hub proteins PPI network. The AC093484.4/TRPV2 interact pair may be involved occurrence development OA.Our study identified several associated characters could provide more information further on

Language: Английский

Regeneration of articular cartilage defects: Therapeutic strategies and perspectives DOI Creative Commons
Xueqiang Guo,

Lingling Xi,

Mengyuan Yu

et al.

Journal of Tissue Engineering, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 1, 2023

Articular cartilage (AC), a bone-to-bone protective device made of up to 80% water and populated by only one cell type (i.e. chondrocyte), has limited capacity for regeneration self-repair after being damaged because its low density, alymphatic avascular nature. Resulting repair defects, such as osteoarthritis (OA), is highly challenging in clinical treatment. Fortunately, the development tissue engineering provides promising method growing cells using hydrogels or porous scaffolds. In this paper, we review therapeutic strategies AC including current treatment methods, engineering/regenerative strategies, recent advances biomaterials, present emphasize on perspectives gene regulation therapy noncoding RNAs (ncRNAs), circular RNA (circRNA) microRNA (miRNA).

Language: Английский

Citations

56
m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis DOI Creative Commons
Jia‐Shu Yang, Ming Zhang, Dawei Yang

et al.

Experimental & Molecular Medicine, Journal Year: 2021, Volume and Issue: 53(11), P. 1723 - 1734

Published: Nov. 1, 2021

Long noncoding RNAs (lncRNAs) have emerged as important regulators of osteoarthritis (OA), but the biological roles and clinical significance most lncRNAs in OA are not fully understood. Microarray analysis was performed to identify differentially expressed lncRNAs, mRNAs, miRNAs between normal osteoarthritic cartilage. We found that AC008440.5 (abbreviated AC008), well AQP1 ANKH, were highly cartilage, whereas miR-328-3p at a low level Functional assays showed ectopic expression AC008, AQP1, ANKH significantly decreased chondrocyte viability promoted apoptosis extracellular matrix (ECM) degradation, knockdown resulted opposite effects. Moreover, overexpression increased attenuated ECM inhibition Bioinformatics analysis, RNA immunoprecipitation (RIP), luciferase revealed AC008 functioned competing endogenous (ceRNA) regulate miR-328-3p, which specifically targeted genes. In addition, ameliorated MIA-induced OA, accelerated progression vivo. Furthermore, fat mass obesity-associated (FTO)-mediated N6-methyladenosine demethylation downregulated transcription, while lower FTO led upregulation transcription OA. conclusion, our data reveal plays critical role pathogenesis via miR-328-3p‒AQP1/ANKH pathway, suggesting may be potential therapeutic target for

Language: Английский

Citations

53
The molecular mechanisms of glycosaminoglycan biosynthesis regulating chondrogenesis and endochondral ossification DOI
Yongjian Chen, Khalid Mehmood, Yung‐Fu Chang

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 335, P. 122243 - 122243

Published: Nov. 8, 2023

Language: Английский

Citations

17
LncRNA SNHG1 enhances cartilage regeneration by modulating chondrogenic differentiation and angiogenesis potentials of JBMMSCs via mitochondrial function regulation DOI Creative Commons
Hua Liu, Huina Liu,

Qiubo Yang

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 18, 2024

Cartilage is a kind of avascular tissue, and it difficult to repair itself when damaged. In this study, we investigated the regulation chondrogenic differentiation vascular formation in human jaw bone marrow mesenchymal stem cells (h-JBMMSCs) by long-chain noncoding RNA small nucleolar host gene 1 (SNHG1) during cartilage tissue regeneration. JBMMSCs were isolated from jaws via adherent method. The effects lncRNA SNHG1 on vitro detected real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Pellet experiment, Alcian blue staining, Masson's trichrome modified Sirius red staining. RT-qPCR, matrix gel tube formation, coculture experiments used determine effect angiogenesis vitro. A model knee defects New Zealand rabbits subcutaneous rubber suppositories nude mice constructed for vivo experiments. Changes mitochondrial function dihydroethidium (DHE) MitoSOX tetramethyl rhodamine methyl ester (TMRM) adenosine triphosphate (ATP) detection. Western blotting was detect phosphorylation level signal transducer activator transcription 3 (STAT3). Red staining showed that promoted differentiation. microvessels vivo. regeneration rabbit tissue. blot alcian JAK inhibitor reduced increase STAT3 deepening caused SNHG1. Mitochondrial correlation analysis revealed led decrease reactive oxygen species (ROS) levels, an membrane potential ATP levels. ROS significantly alleviated knockdown. promotes JBMMSCs. regulates STAT3, reduces ROS, energy metabolism, ultimately

Language: Английский

Citations

5
Elucidating the role of ubiquitination and deubiquitination in osteoarthritis progression DOI Creative Commons

Chenxiao Zheng,

Jiayi Chen, Yurui Wu

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 9, 2023

Osteoarthritis is non-inflammatory degenerative joint arthritis, which exacerbates disability in elder persons. The molecular mechanisms of osteoarthritis are elusive. Ubiquitination, one type post-translational modifications, has been demonstrated to accelerate or ameliorate the development and progression via targeting specific proteins for ubiquitination determining protein stability localization. Ubiquitination process can be reversed by a class deubiquitinases deubiquitination. In this review, we summarize current knowledge regarding multifaceted role E3 ubiquitin ligases pathogenesis osteoarthritis. We also describe insight into processes. Moreover, highlight multiple compounds that target influence progression. discuss challenge future perspectives modulation expression enhancement therapeutic efficacy patients. conclude modulating deubiquitination could alleviate achieve better treatment outcomes

Language: Английский

Citations

12
Sirt1 alleviates osteoarthritis via promoting FoxO1 nucleo-cytoplasm shuttling to facilitate autophagy DOI
Mao Xu, Zhuang Qian, Ying Zhang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 131, P. 111893 - 111893

Published: March 20, 2024

Language: Английский

Citations

4
Study on Transorgan Regulation of Intervertebral Disc and Extra-Skeletal Organs Through Exosomes Derived From Bone Marrow Mesenchymal Stem Cells DOI Creative Commons
Zhikun Wang,

Yangming Wu,

Zhonghan Zhao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: Sept. 23, 2021

Exosomes are membranous lipid vesicles fused with intracellular multicellular bodies and then released into the extracellular environment. They contain various bioactive substances, including proteins, mRNA, miRNAs, lncRNAs, circRNAs, lipids, transcription factors, cytokine receptors. Under certain conditions, bone marrow mesenchymal stem cells (BMSCs) can differentiate osteoblasts, chondrocytes, adipocytes, biological functions. This study provides a theoretical basis for application of exosomes derived from (BMSC-Exos) in osteology, exploring different sources to improve microenvironment resist metastasis. We also provided new ideas prevention rehabilitation human diseases by exosomes.

Language: Английский

Citations

25
Carvacrol induces osteogenic differentiation of BMSCs and alleviates osteolysis in aged mice by upregulating lncRNA NEAT1 to promote SIRT1 expression DOI Creative Commons
Zefeng Xu, Zeyu Wang,

Wenming Ma

et al.

Aging Pathobiology and Therapeutics, Journal Year: 2023, Volume and Issue: 5(4)

Published: Dec. 20, 2023

Background: Wear particle-induced periprosthetic osteolysis is a major contributor to joint replacement failure and revision surgery in the elderly. Osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) promising approach for regeneration. Carvacrol may have bone-protective potential. This study investigated whether carvacrol alleviates promotes osteogenic BMSCs by regulating SIRT1 expression. Materials Methods: An model aged mice was established titanium (Ti) particle induction. were isolated from femur tibia 18-month-old cultured medium. RIP RNA pull-down used evaluate binding lncRNA NEAT1. Ubiquitination analysis performed investigate NEAT1-mediated regulation ubiquitination modification levels. Results: treatment improved Ti calvarial erosion attenuated osteolysis. increased both mouse Ti-induced under differentiation. promoted ALP activity, mineralization capacity, expression differentiation-related factors, whereas knockdown reversed this effect. LncRNA NEAT1 interacted with SIRT1, overexpression stabilized inhibiting its modification. altered promoting effect on BMSCs, while effects knockdown. Similarly, inhibitory vivo . Conclusion: Our research results demonstrate that showed potential treating promote can facilitate BMSCs. Keywords: Osteolysis, carvacrol, cells, NEAT1,

Language: Английский

Citations

10
NLRP3 Inflammasome-Mediated Osteoarthritis: The Role of Epigenetics DOI Creative Commons
Yuzhou Liu, Ying Wang,

Ping Yan

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 71 - 71

Published: Jan. 14, 2025

The prevalence of osteoarthritis (OA) notably surges with age and weight gain. most common clinical therapeutic drugs are painkillers, yet they cannot impede the deteriorating course OA. Thus, understanding OA's pathogenesis devising effective therapies is crucial. It generally recognized that inflammation, pyroptosis, OA progression tightly linked. activation NLRP3 inflammasome can lead to discharge pro-inflammatory cytokines Interleukin-1β IL-18, intensifying subsequent inflammatory reactions promoting development. Conversely, imbalance caused by deacetylase-regulated underlies chronic mild inflammation related degenerative diseases. Therefore, this article expounds on mechanism role histone deacetylases (HDACs) in inflammasome-triggered OA, illustrates application HDAC inhibitors striving provide more insights into novel treatment approaches.

Language: Английский

Citations

0
Therapeutic potential of SOX family transcription factors in osteoarthritis DOI Creative Commons
Yue Huang, Zhuo Wang

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: Jan. 31, 2025

Background As the worldwide population ages, osteoarthritis has significantly increased. This musculoskeletal condition become a pressing global health issue and thus, prevention treatment of have primary focus domestic international research. Scholarly investigations molecular mechanisms that are related to occurrence development shed light on pathological causes this certain extent, providing foundation for its treatment. However, further research is necessary fully understand critical role transcription factor SOX9 in chondrocyte differentiation osteoarthritis. result, there been widespread interest SOX factors. While utilized as biomarker indicate prognosis osteoarthritis, into other members family targeted treatments around still required.

Language: Английский

Citations

0