New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

Poultry Science, Journal Year: 2024, Volume and Issue: 103(6), P. 103674 - 103674

Published: March 16, 2024

Microplastics biological toxicity, environmental persistence and chemicals have been paid widespread attention. exposed to chicken spleen injury of the specific mechanism is unclear. Thus, we randomly assigned chickens 4 groups: C (normal diet), L-MPs (1 mg/L), M-MPs (10 H-MPs (100 assessed damage after 42 d exposure. Morphologically, boundary between red white pulp was blurred, along with expansion pulp. It further speculated that microplastics induced mitochondrial dynamic homeostasis (Drp1 upgraded, Mfn1, Mfn2, OPA1 reduced), provoked apoptotic pathway (Bcl-2/Bax decreased, cytc, caspase3, caspase9 raised), resulting in redox imbalance lipid peroxide accumulation (MDA increased, CAT, GSH, T-AOC plummeted), stimulated ferroptosis (FTH1, GPX4, SLC7A11 decreased). Here explored impact polystyrene on spleen, as well programmed death (apoptosis ferroptosis) involved, regulative role mitochondria this process. This could be significant importance bridging gap laboratory research microplastics-induced chicken.

Language: Английский

---

Chronic degenerative failure of salivary glands can be reversed through restoring mitochondrial function

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract There are no therapies for reversing chronic organ degeneration. Non-healing degenerative wounds thought to be irreparable, in part, by the inability of tissue respond reparative stimuli. As such, treatments typically aimed at slowing degeneration or replacing cells through transplantation. Building on our previous studies showing acutely injured salivary glands, and specifically secretory acini, can regenerated, we reveal that non-healing, degenerating murine glands remain responsive a neuromimetic (muscarinic) agonist with treatment resulting restoration tissue. Not only is degenerated structure function returned homeostatic-like state, but this outcome also sustained months after termination. Furthermore, despite an eventual reduction saliva secretion, gland responds second round treatment, fully regaining resembles uninjured controls. Our findings suggest rescue due reversal aberrant de-differentiated acinar cell state mitochondrial dysfunction muscarinic-calcium signaling pathway. Thus, these data challenge concept irreversible provides readily testable therapeutic strategy epithelial may significantly benefit diversity disease conditions.

Language: Английский

---

Ferroptosis at the crossroads: Insights and advances in non-neoplastic pancreatic diseases

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 158, P. 114870 - 114870

Published: May 17, 2025

Ferroptosis is a form of regulated cell death characterized by iron accumulation and increased lipid peroxidation, primarily counteracted range antioxidant molecules, including glutathione (GSH), peroxidase 4 (GPX4), ubiquinone, tetrahydrofolate, nuclear respiratory factor 2. Furthermore, the process ferroptosis intricately influenced opposing actions p53 tumor suppressor gene activated transcription factors 3 4, which can either facilitate or hinder ferroptotic depending on cellular context. This significantly associated with various pancreatic disorders, both acute chronic pancreatitis, as well diabetes mellitus. In this review, we thoroughly investigate mechanisms underlying ferroptosis, focusing overload, regulatory molecules involved in modulation (notably system xc-/GSH/GPX4 axis), along relevant signaling pathways. We also examine role non-neoplastic diseases such pancreatitis mellitus while identifying novel therapeutic agents that target potentially paving way for innovative treatment strategies conditions.

Language: Английский

---

Network pharmacology analysis reveals that coumestrol targets ZYX to inhibit ferroptosis and alleviate acute pancreatitis

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 159, P. 114948 - 114948

Published: May 24, 2025

Language: Английский

---

Unveiling the intersection: ferroptosis in influenza virus infection

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 12, 2024

The influenza virus (IFV) imposes a considerable health and economic burden globally, requiring comprehensive understanding of its pathogenic mechanisms. Ferroptosis, an iron-dependent lipid peroxidation cell death pathway, holds unique implications for the antioxidant defense system, with possible contributions to inflammation. This exploration focuses on dynamic interplay between ferroptosis host against viruses, emphasizing influence IFV infections activation pathway. causes different types death, including apoptosis, necrosis, ferroptosis. IFV-induced ferroptotic is mediated by alterations in iron homeostasis, intensifying accumulation reactive oxygen species promoting peroxidation. A investigation into mechanism viral infections, specifically IFV, has great potential identify therapeutic strategies. may pave way development drugs using inhibitors, presenting effective approach suppress infections.

Language: Английский

---

mTORC2 knockdown mediates lipid metabolism to alleviate hyperlipidemic pancreatitis through PPARα

Journal of Biochemical and Molecular Toxicology, Journal Year: 2024, Volume and Issue: 38(8)

Published: Aug. 1, 2024

Abstract Hyperlipidemic pancreatitis (HP) is an inflammatory injury of the pancreas triggered by elevated serum triglyceride (TG) levels. The mechanistic target rapamycin (mTOR) signaling pathway plays a crucial role in regulating lipid homeostasis and inflammation. This study aimed to investigate whether activity mTOR complex 2 (mTORC2) affects progression HP its underlying mechanisms. In vivo, high‐fat diet retrograde administration sodium taurocholate were employed establish models rats, with pancreatic tissue pathology evaluated. expression Rictor peroxisome proliferator‐activator receptor (PPAR) was examined. levels TG, fatty acid metabolites, metabolism‐related factors determined. vitro, acinar cells (PACs) exposed palmitic cholecystokinin‐8. PAC apoptosis, pyroptosis, ferroptosis assessed. models, rats PACs exhibited upregulated downregulated PPARα, knockdown promoted PPARα expression. decreased α‐amylase, total cholesterol, low‐density lipoprotein lactate dehydrogenase, factors, while increasing high‐density cholesterol increased ACOX1 CPT1α SREBP‐1, CD36, SCD1, ACLY, ACACA. reduced damage structure. inhibited ferroptosis. Treatment antagonist GW6471 abolished beneficial effects knockdown. Rictor/mTORC2 deficiency reduces TG levels, maintains homeostasis, suppresses inflammation inhibiting Weakening mTORC2 holds promise as novel therapeutic strategy for HP.

Language: Английский

---

Ion channels in acinar cells in acute pancreatitis: crosstalk of calcium, iron, and copper signals

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 13, 2024

The initial stages of acute pancreatitis (AP) are characterized by a significant event - acinar ductal metaplasia (ADM). This process is crucial feature both and chronic pancreatitis, serving as the first step in development pancreatic cancer. Ion channels integral transmembrane proteins that play pivotal role numerous biological processes modulating ion flux. In many diseases, expression activity often dysregulated. Metal ions, including calcium ions (Ca 2+ ), ferrous (Fe Copper (Cu assume distinctive cellular metabolism. These possess specific properties relevant to function. However, interactions among these exacerbate imbalance within intracellular environment, resulting damage influencing progression AP. A more in-depth investigation into mechanisms which interact with cells essential for elucidating AP’s pathogenesis identifying novel therapeutic strategies. Currently, treatment AP primarily focuses on pain relief, complications prevention, prognosis improvement. There limited treatments targeting acinous cell dedifferentiation or imbalance. study aims investigate potential strategies examining crosstalk context pancreatitis.

Language: Английский

---

A D–π–A-type ratiometric fluorescent probe to detect polarity changes and inhibition effect during ferroptosis

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(22), P. 3486 - 3491

Published: Jan. 1, 2024

To thoroughly understand ferroptosis's biological functions in living cells, it is crucial to investigate the polarity variations that occur during this unique Fe(II)-facilitated oxidative type of cell death. In work, we report development a ratiometric probe (Po-P) visualize changes cells and inhibition effect ferroptosis. The polarity-responsive fluorophore utilized by Po-P has D-π-A-type structure. Based on theoretical calculations, ICT was proposed as basis for Po-P's mechanism. According imaging results, had desirable capacity monitoring fluctuations erastin-induced Furthermore, revealed dihydrolipoic acid (DHLA) could potentially prevent ferroptosis, just vitamin E (VE). We anticipate be valuable tool quickly monitor effects ferroptosis create new medications treating disorders related

Language: Английский

---

Multi-Dimensional Analysis of the Regulatory Network of Ferroptosis in Osteoarthritis: Progress, Challenges, and Future Directions

Published: Jan. 1, 2024

Language: Английский

---

Metabolic complementation between cells drives the evolution of tissues and organs

Biology Letters, Journal Year: 2024, Volume and Issue: 20(11)

Published: Nov. 1, 2024

Although evolutionary transitions of individuality have been extensively theorized, little attention has paid to the origin levels organization within organisms. How and why do specialized cells become organized into tissues or organs? What spurs a transition in organizational level cases where function is already present constituent cell types? We propose hypothesis for this kind based on two features cellular metabolism: metabolic constraints functional performance capacity complementation between parenchymal supporting cells. These suggest scenario whereby pre-existing types are integrated when changes internal external environment favour offloading burdens from primary type onto illustrate process ‘supra-functionalization’ using nervous system pancreas.

Language: Английский

---